Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 1 de 1
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Rev Inst Med Trop Sao Paulo ; 36(6): 531-7, 1994.
Artigo em Inglês | MEDLINE | ID: mdl-7569628

RESUMO

A dot-enzyme-linked immunosorbent assay (Dot-ELISA) for pneumococcal antigen detection was standardized in view of the need for a rapid and accurate immunodiagnosis of acute pneumococcal pneumonia. A total of 442 pleural fluid effusion samples (PFES) from children with clinical and laboratory diagnoses of acute bacterial pneumonia, plus 38 control PFES from tuberculosis patients and 20 negative control serum samples from healthy children were evaluated by Dot-ELISA. The samples were previously treated with 0.1M EDTA pH 7.5 at 90 degrees C for 10 min and dotted on nitrocellulose membrane. Pneumococcal omniserum diluted at 1:200 was employed in this assay for antigen detection. When compared with standard bacterial culture, counterimmunoelectrophoresis and latex agglutination techniques, the Dot-ELISA results showed relative indices of 0.940 to sensitivity, 0.830 to specificity and 0.760 to agreement. Pneumococcal omniserum proved to be an optimal polyvalent antiserum for the detection of pneumococcal antigen by Dot-ELISA. Dot-ELISA proved to be a practical alternative technique for the diagnosis of pneumococcal pneumonia.


Assuntos
Antígenos de Bactérias/análise , Immunoblotting , Derrame Pleural/química , Pneumonia Pneumocócica/diagnóstico , Polissacarídeos Bacterianos/análise , Streptococcus pneumoniae/imunologia , Doença Aguda , Criança , Pré-Escolar , Contraimunoeletroforese , Humanos , Lactente , Recém-Nascido , Testes de Fixação do Látex , Pneumonia Bacteriana/diagnóstico , Valor Preditivo dos Testes , Sensibilidade e Especificidade
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...