The Influence of Relaxing and Self-Selected Stimulating Music on Vertical Jump Performance in Male Volleyball Players.

Most research concerning the effects of music on physical performance was conducted using endurance parameters. This study investigated the effects of relaxing (RLX) vs. self-selected stimulating music (SM) vs. no music (NM) on jump height (JH), jump power (PWR), and average rest period between jumps (RP) in 13 athletes (age: 25.5 ± 2.6 years). After a warm-up and listening to music (1 min) or NM, participants completed five squat jumps on a force plate. Psychological ratings of mood were assessed using a questionnaire before warm-up and after jumping. A one-way ANOVA was conducted to compare effects of music on JH, PWR, and RP. A Friedman test with Wilcoxon signed-rank test was used to detect changes in mood. There were no significant effects of music on JH (p = 0.162) and PWR (p = 0.162). A trend towards longer RP in RLX when compared to SM was detected (+2.72 s, +22%, p = 0.059, d = 0.35). Participants felt more "relaxed" (+3 ranks) and more "powerful" after listening to SM (+2 ranks). Following NM and RLX, athletes felt more "energetic" (each +3 ranks) but less energetic (-3 ranks) after SM. In conclusion, this study did not find any performance-enhancing effects of self-selected SM on jump performance. The influences of music on psychological ratings were inconclusive. For this reason, no evidence-based guidelines for the practical application of music in elite jumping athletes can be made, and more studies are warranted.

Paediatric trauma: injury pattern and mortality in the UK.

OBJECTIVE: Trauma accounts for a large proportion of childhood deaths. No data exist about injury patterns within paediatric trauma in the UK. Identification of specific high-risk injury patterns may lead to improved care and outcome. METHODS: Data from 24 218 paediatric trauma cases recorded by the Trauma Audit and Research Network (TARN) from 1990 to 2005 were analysed. Main injury, injury patterns and outcome were analysed. Mortality at 93 days' post-injury was the major outcome measure. RESULTS: Limb injuries occurred in 65.0% of patients. In infants 81.4% of head injuries were isolated, compared with 46.5% in 11-15-year-old children. Thoracic injuries were associated with other injuries in 68.4%. The overall mortality rate was 3.7% (n = 893). Mortality decreased from 4.2% to 3.1%; this was most evident in non-isolated head injuries. It was low in isolated injuries: 1.5% (n = 293). In children aged 1-15 years the highest mortalities occurred in multiple injuries including head/thoracic (47.7%) and head/abdominal injuries (49.9%). Having a Glasgow Coma Scale of <15 on presentation to hospital was associated with a mortality of 16%. CONCLUSIONS: Differences in injury patterns and mortality exist between different age groups and high-risk injury patterns can be identified. With increasing age, a decline in the proportion of children with head injury and an increase in the proportion with limb injury were observed. This information is useful for directing ongoing care of severely injured children. Future analyses of the TARN database may help to evaluate the management of high-risk children and to identify the most effective care.

[New condylar head system for temporary condylar reconstruction in ablative tumour surgery]. / Neu entwickeltes Kiefergelenkkopfimplantat zur temporären Kondylus-Rekonstruktion in der ablativen Tumorchirurgie.

BACKGROUND: Based on own retrospective studies a condylar head add-on system for immediate, temporary reconstruction in patients undergoing ablative surgery requiring the removal of the manibular condyle has been developed in cooperation with the Department of Oral and Maxillofacial Surgery of the University of Tennessee, USA, and the Association for the Study of Internal Fixation (AO/ASIF). PURPOSE: The design of the new condylar head add-on system and its use in an anatomical dissection study on a human cadaver are introduced and discussed. DESIGN AND FIRST EXPERIENCES: The condylar replacement is made of commercial pure titanium and is conceived as an add-on system. It consists of a reconstruction plate (2.4 Uni-LOCK-System) und an adaptable condylar head that can be fitted on either side. The offset of the condylar head in a medial direction allows anatomically correct positioning of the implant. The slanted oval head shall provide a large contact area while maintaining function of the mandibular joint. The height-adjustable positioning of the condylar head add-on with four different fixations plates facilitates an intraoperative vertical correction of the condylar head without necessary bending of a new reconstruction plate. A condylar head add-on used on both sides and combined with the frequently used 2.4 Uni-LOCK-plate benefits from reduced storekeeping and turns out to be advantageous from an economic point of view. PERSPECTIVE: An international, prospective multi-center study evaluating the intraoperative applicability of the new condylar head add-on system and its functional as well as aesthetic results during the first two postoperative years has started in September 2006.

Watson fundoplication in children: a comparative study with Nissen fundoplication.

BACKGROUND: Nissen fundoplication is the gold standard antireflux procedure in children. In 1996, one pediatric surgeon adopted the anterior fundoplication described by Watson in 1991. This procedure is reported to achieve good reflux control while permitting burping, active vomiting, and reducing gas bloat. An audit project was undertaken to compare the clinical outcome of children undergoing Nissen and Watson fundoplication. METHODS: The case notes of 144 children undergoing open fundoplication between February 1995 and February 2002 were reviewed retrospectively. RESULTS: Results of 72 boys and 59 girls comprising 76 Nissen and 55 Watson fundoplications were assessed. In each group, one death occurred within 1 month of operation. Chest infections occurred in 6.6% (Nissen) and 1.8% (Watson), and wound infections in 2.6% and 1.8%, respectively. Dysphagia was recorded in 7.9% of Nissen and 1.8% of Watson fundoplications. Follow-up data were analyzed in 70 children with Nissen and 48 children with Watson fundoplication. When overall clinical outcome was assessed for those patients with a minimum follow-up of 1 year, 85.1% Nissen and 88.2% Watson were judged good/excellent; 14.9% Nissen and 11.8% Watson were judged poor/bad. CONCLUSION: Watson fundoplication can safely be performed in children with comparable clinical outcome to Nissen fundoplication.

Colonic transit time--what is normal?

BACKGROUND: Constipation is a common problem in childhood, and various radiologic methods have been advocated for investigation. Colonic transit time (CTT) has been used in adults to investigate colonic motility, but few studies evaluate this method in children. Data on CTT in the normal paediatric population are scarce. METHODS: The colonic transit time was measured in 22 healthy children (median age, 10 years; range, 4 to 15 years) by Abrahamsson's method. Children took bolus ingestions of radiopaque markers on 6 consecutive days, and on day 7 a single abdominal x-ray was performed. This was evaluated for total and segmental colonic transit time. RESULTS: The mean total CTT was 40 hours, and the upper limit of normal (95th percentile) was 84 hours. The upper limit of normal for segmental transit time was as follows: 14 hours for the ascending, 33 hours for the transverse, 21 hours for the descending, and 41 hours for the rectosigmoid colon. CONCLUSIONS: CTT provides an objective measure to assess childhood constipation. To date, 6 studies using 5 different methods have been published reporting values for healthy children. Comparing these, Abrahamson's method has low radiation exposure and is well tolerated. This study contributes additional normal values in children.

Chronic disease self-management: improving health outcomes.

Chronic disease has become pandemic in the United States, and estimates are that it will affect 148 million people by the year 2030. Patients with chronic illnesses cost the health care system over three times more than individuals without chronic conditions. The US Department of Veterans' Affairs (VA) Sunshine HealthCare Network, composed of VA health care facilities in Florida and Puerto Rico, recognized that the needs of its increasing number of veterans with chronic diseases were unmet by traditional medical interventions. The Network implemented a chronic disease self-management pilot program to evaluate its value for the veteran population. Results of the pilot indicate that this program will make a positive, lasting change in the health status and quality of life for veterans with chronic disease.

The U3 promoter and the nef gene of simian immunodeficiency virus (SIV) smmPBj1.9 do not confer acute pathogenicity upon SIVagm.

Two chimeric proviruses comprising the U3 promoter and the nef gene of simian immunodeficiency virus (SIV) smmPBj1.9 in addition to other genomic regions of SIVagm3mc from African green monkeys (Cercopithecus aethiops) were constructed. The derived chimeric viruses (SIVagm3mc/SIVsmmPBj1.9) were both able to replicate in nonstimulated peripheral blood leukocytes from pig-tailed macaques (Macaca nemestrina), a biological property often correlated with acute pathogenicity. However, only one of the chimeric viruses was acutely pathogenic, inducing a rapid depletion of the peripheral CD4+ T cells in two infected pig-tailed macaques within 10 days after infection in a manner similar to infection with SIVsmmPBj1.9 itself. The other chimeric virus actively replicated during the first 8 weeks after experimental infection of two pig-tailed macaques but induced neither acute disease nor CD4+ T-cell depletion for 113 weeks after infection. Thus, the U3 promoter and the nef gene of SIVsmmPBj1.9 alone appear to be insufficient to confer acute pathogenicity to SIVagm3mc.

An in vitro assay for acute pathogenicity of immunodeficiency viruses.

As a model for AIDS, experimental infections of old-world monkeys with various simian immunodeficiency viruses (SIV) are frequently carried out to study mechanisms of pathogenicity. For example, SIVsmmPBj14 was isolated from a pig-tailed macaque (Macaca nemestrina) suffering from acute viral disease. The molecular virus clone SIVsmmPBj1.9, which displays close genetic homology to other related SIVs, was shown to induce an acute viral disease in vivo after infection of pig-tailed and rhesus macaques. The acute pathogenicity of SIVsmmPBj1.9 was correlated with its unique ability to replicate in non-stimulated peripheral blood mononuclear cells from pig-tailed macaques. We have exploited this in vitro assay to resolve putative pathogenic genetic determinants of another SIV, namelySIVagm3, isolated from African green monkeys (Cercopithecus aethiops). Hybrid viruses encompassing subgenomic regions of SVsmmPBj1.9 in place of comparable regions of molecular virus clone SIVagm3mc were constructed and tested for their ability to replicate in non-stimulated PBMC from pig-tailed macaques and African green monkeys. Only those hybrid viruses comprising the U3 region of the viral LTR of SIVsmmPBj1.9 replicated in non-stimulated peripheral blood mononuclear cells. This in vitro assay will be used to determine the potential of SIV and of hybrid viruses between different SIVs to induce acute viral disease in vivo. It will help to avoid excessive experimental infections of monkeys with respective hybrid viruses for determining genetic determinants of acute pathogenicity of immunodeficiency viruses.

Induction of antibodies against SIV antigens after intramuscular nucleic acid inoculation using complex expression constructs.

By studying the infection of rhesus macaques with simian immunodeficiency virus (SIVmac) the potential of nucleic acid immunisation against AIDS can be evaluated. As a first step towards the development of suitable expression constructs, the levels and the durations of expression elicited by the house-keeping gene promoters of the murine phospho-glycerate kinase (PGK) gene and rat proto-ras 1Ha, a lentiviral LTR and the CMV-intron A promoter were tested in BALB/c mice intramuscularly inoculated with marker gene constructs encoding luciferase. The expression levels achieved by the CMV-intron A and the lentiviral promoter were comparably high, and also the PGK promoter induced a high level of expression for at least 64 days. Following the inoculation of plasmids comprising single or multiple genes of SIV, the induction of specific antibodies directed against SIV antigens was demonstrated. We previously showed in vitro that int- and nef-defective mutants of SIVmac were able to initiate a limited and self-abortive infection of permissive cells in the absence of chromosomal integration of the viral DNA. Intramuscular inoculations in monkeys using int-defective proviral DNA of SIV will show whether an increased immune response may be induced by expression of viruses undergoing a self-limited replication in vivo.

Modulation of accessory cell function of immortalized bone marrow-derived macrophages by granulocyte/macrophage colony-stimulating factor.

To generate cloned macrophage populations with sensitivity towards granulocyte/macrophage colony-stimulating factor (GM-CSF), bone marrow-derived macrophages (BMM phi) were immortalized by transformation with SV40. A panel of transformed clones was established. The majority of clones represented independently derived transformants, as evidenced by restriction fragment length polymorphism using genomic DNA digested with EcoRI and TaqI and the 5.2 kb SV40 DNA for hybridization analysis. The cells belong to the macrophage lineage according to several criteria, e.g. the presence of nonspecific esterase, their phagocytic capacity and their morphology. Many clones were potent antigen-presenting cells (APC), without exogenous stimulation. Two clones, which did not act efficiently as APC when used untreated, were positively responsive to GM-CSF. GM-CSF stimulation of both clones resulted in potent APC capacity. I-A alpha, I-A beta and gamma chain-specific transcripts were observed upon stimulation with GM-CSF, corresponding to detectable levels of class II surface display as revealed by cytofluorometric analysis. Thus the macrophage clones established will allow dissection of the differential effects of GM-CSF on the parameters of antigen presentation.

Utilization of various chemotrophic and phototrophic bacteria as food by the anaerobic ciliate Trimyema compressum.

A monoxenic culture of the anaerobic ciliate Trimyema compressum growing on Bacteroides strain WoCb 15 was tested for growth on 15 chemotrophic and 27 phototrophic bacterial strains as sole food bacteria. Apart from Bacteroides strain WoCb 15, which yielded the highest cell numbers of Trimyema, only 4 species of Gram-negative chemotrophic bacteria and 7 species (13 strains) of phototrophic bacteria (all Gram-negative) supported multiplication of Trimyema. No growth could be obtained on 7 different strains of Gram-positive bacteria. The dry cell mass yield of Trimyema amounted to 6.1% of the dry cell mass of Bacteroides strain WoCb 15 consumed. As a result, only 0.34% of the carbon in the organic substrate (tartrate) consumed for growth and fermentation of Bacteroides strain WoCb 15 ended up in the carbon content of Trimyema. The low yields are due to the fermentative metabolism of both organisms. Trimyema ceased to multiply after consumption of Bacteroides cells down to a number of 1 · 10(7) cells/ml.

Blastocystis hominis--a potential intestinal pathogen.

The parasite Blastocystis hominis has been found in 10% to 18% of stool specimens submitted to microbiology laboratories. Controversy exists as to whether this organism can cause illness in humans. We have reviewed the records of 65 symptomatic patients with B hominis in their stool. We conclude that B hominis is a potential pathogen that may or may not require drug therapy depending on the overall clinical circumstances, the severity of symptoms, and the presence of other pathogenic organisms.

[The Ha(La) antibody as a serologic marker of systemic lupus erythematosus]. / Der Ha(La)-Antikörper als serologische Kenngrösse des systemischen Lupus erythematodes (SLE).

Ha(La)-antibodies are detected by counterimmunoelectrophoresis (CIE) in sera of systemic lupus erythematosus (SLE) patients, but not in sera of patients with progressive systemic scleroderma (PSS) (n = 38), rheumatoid arthritis (n = 91), ankylosing spondylitis (n = 97), and other rheumatic diseases (n = 452). This antibody specificity was found in 27 out of 103 SLE patients (26%). However, 50% of SLE patients with Sjögren's syndrome (n = 12) showed the Ha(La)-antibody, suggesting a strong association in this clinical subgroup. Thus the Ha(La)-antibody can serve as a serological marker and helpful diagnostic tool for SLE. Additionally, the Ha(La)-antibody reacts with specific puffs in polytene chromosome preparations of Drosophila melanogaster. Therefore, this heterologous antigen system is suitable for the identification of Ha(La) sera.

Fermentative degradation of nonionic surfactants and polyethylene glycol by enrichment cultures and by pure cultures of homoacetogenic and propionate-forming bacteria.

Linear alkyl ethoxylates (polyethylene glycol alkyl ethers) were fermented completely to methane and CO2 in enrichment cultures inoculated with anoxic sewage sludge. Long-chain fatty acids were released as intermediates. No degradation was found with polypropylene glycol and polypropylene glycol-containing surfactants. Two types of primary ethoxylate-degrading bacteria were isolated and characterized. Both degraded polyethylene glycols with molecular weights of 1,000 completely. Strain KoB35 fermented polyethylene glycol, ethoxyethanol, and lactate to acetate and propionate and was assigned to the described species Pelobacter propionicus. Strain KoB58 converted polyethylene glycol and many other substrates to acetate only and was assigned to the genus Acetobacterium. The pathways of anaerobic degradation of nonionic surfactants are discussed with respect to their limitations and the various groups of bacteria involved.