NATURALLY OCCURRING RADIONUCLIDE 40K BODY CONTENT OF KAKRAPAR GUJARAT SITE POPULATION.

An attempt was made to investigate the naturally occurring radionuclide 40K body content of the Kakrapar Gujarat Site (India) population by using whole-body counting method. The population comprises occupational workers (regular and contract personnel working at Kakrapar Atomic Power station) and students from various educational institutions. A total of 1056 subjects of different age groups and gender were monitored for 40K body content by using a Standfast-II Whole Body Counting System. The reactor released radionuclides such as 137Cs, 60Co and 131I are not detected in occupational workers of Kakrapar Atomic Power station which indicates that occupational workers are free from radioactive contamination. Based on the activity measurement, an effective dose due to 40K was estimated for different groups of the site population based on the dietary habits, sex and age. Statistical significant correlation was not observed in any of the groups. The annual effective dose due to 40K was found to be in the range of 52-243 µSv y-1 with an average of 133 ± 35 µSv y-1 for the vegetarian group (588 subjects), 60-223 µSv y-1 with an average of 136 ± 35 µSv y-1 for the non-vegetarian group (468 subjects), 52-243 µSv y-1 with an average of 134 ± 35 µSv y-1 for the male group (1007 subjects) and 67-203 µSv y-1 with an average of 128 ± 35 µSv y-1 for the female group (49 subjects), respectively. Among the four age groups of the population studied, an effective dose due to 40K is slightly higher in the age group of 20-29 (161 subjects). The annual effective dose due to 40K reported by UNSCEAR, 1982(3) is 180 µSv y-1.

A Single Amino Acid Substitution in the Intervening Region of 129K Protein of Cucumber Green Mottle Mosaic Virus Resulted in Attenuated Symptoms.

Cucumber green mottle mosaic virus (CGMMV), a member of the genus Tobamovirus, is a major threat to economically important cucurbit crops worldwide. An attenuated strain (SH33b) derived from a severe strain (SH) of CGMMV caused a reduction in the viral RNA accumulation and the attenuation of symptoms, and it has been successfully used to protect muskmelon plants against severe strains in Japan. In this study, we compared GFP-induced silencing suppression by the 129K protein and the methyltransferase domain plus intervening region (MTIR) of the 129K protein between the SH and SH33b strains, respectively. As a result, silencing suppression activity (SSA) in the GFP-silenced plants was inhibited efficiently by the MTIR and 129K protein of SH strain, and it coincided with drastically reduced accumulation of GFP-specific small interfering RNAs (siRNAs) but not by that of SH33b strain. Furthermore, analyses of siRNA binding capability (SBC) by the MTIR of 129K protein and 129K protein using electrophoretic mobility shift assay revealed that SBC was found with the MTIR and 129K protein of SH but not with that of SH33b, suggesting that a single amino acid mutation (E to G) in the MTIR is responsible for impaired SSA and SBC of SH33b. These data suggest that a single amino acid substitution in the intervening region of 129K protein of CGMMV resulted in attenuated symptoms by affecting RNA silencing suppression.

Mitigation of harmonics and unbalanced source voltage condition in standalone microgrid: positive sequence component and dynamic phasor based compensator with real-time approach.

Penetration of Distributed Energy Resources (DER) is in high demand to supply power to the load where the grid is not available. Many of these sources are a single phase source used to form standalone Microgrid (MG). Single phase connectivity of these sources results in an unbalanced source voltage condition (UbSVC). Interfacing power electronic devices also inject the harmonics into Point of Common Coupling (PCC) voltage. The effect of this unbalance and harmonics on the operation of standalone MG is analysed in this paper in a twofold manner. One at a reduced power transfer from DER to load and the other is an error produced in Phase Locked Loop (PLL) operation. Positive Sequence Component (PSC) based and Dynamic Phasor (DP) based compensation techniques are proposed in this paper to mitigate the effect of UbSVC. Simulation validates that both the proposed methods are capable to provide balanced load voltage condition under UbSVC in terms of Voltage Unbalance Factor ( V U b F ). It also enhances the power transferred from DER to load during an UbSVC. The performance of the proposed compensator during UbSVC and harmonic presence is validated in real-time simulation using Opal-RT and dSPACE simulators.

Functional annotation of the genome unravels probiotic potential of Bacillus coagulans HS243.

Spore forming Bacillus species are widely used as probiotics for human dietary supplements and in animal feeds. However, information on genetic basis of their probiotic action is obscure. Therefore, the present investigation was undertaken to elucidate probiotic traits of B. coagulans HS243 through its genome analysis. Genome mining revealed the presence of an arsenal of marker genes attributed to genuine probiotic traits. In silico analysis of HS243 genome revealed the presence of multi subunit ATPases, ADI pathway genes, chologlycine hydrolase, adhesion proteins for surviving and colonizing harsh gastric transit. HS243 genome harbored vitamin and essential amino acid biosynthetic genes, suggesting the use of HS243 as a nutrient supplement. Bacteriocin producing genes highlighted the disease preventing potential of HS243. Thus, this work established that HS243 possessed the genetic repertoire required for surviving harsh gastric transit and conferring health benefits to the host which were further validated by wet lab evidences.

Evaluation of tear film stability before and after laser in situ keratomileusis.

OBJECTIVE: The study was conducted to evaluate tear film stability and tear secretion before and after laser in situ keratomileusis. MATERIALS AND METHODS: It was a prospective, longitudinal and non-comparative analysis of clinical data of 20 consecutive myopic patients (40 eyes) collected before and after laser in situ keratomileusis. Assessments included tear secretion (Schirmer I and II), fluorescein tear break up time and ocular surface staining. STATISTICS: The statistical package for social science (SPSS 10.0) was used for data analysis. The parameters of tear secretion and tear stability were analyzed using the paired and unpaired Student t-tests. RESULTS: Schirmer II was reduced at seven days (9.5 ± 4.30 mm) and one month (10.3 ± 3.06 mm, p=0.001) after operation from the pre-operative value of 16.12 ± 3.90 mm. Tear film stability significantly decreased at seven days (6.79 ± 3.05 sec, p Less than 0.001) and one month (8.03 ± 2.81secs, p less than 0.001) from its pre-operative value (12.68 ± 2.69 secs). 87.5% had tear film instability (FBUT less than 10secs) seven days after surgery; it was reduced to 75 % at one month and 27.5 % at three months. It was 7.5 % before surgery. Corneal staining score was increased significantly at seven days (1.42 ± 1. 58, p less than 0.01) and one month (0.95 ± 1.41, p=0.02), from the pre-operative score of 0.17 ± 0.44. CONCLUSION: Laser in situ keratomileusis significantly alters the tear film stability, Schirmer values and corneal staining at least for three months.

Assessment of daily intake of trace elements by Kakrapar adult population through ingestion pathway.

Concentration of trace elements such as Fe, Cu, Ni, and Zn were measured in cereals, pulses, vegetables, fish, meat, milk, egg, and water samples collected around Kakrapar, Gujarat, India. A wide variation of the trace element concentration was observed among all the dietary matrices. The concentration of Fe is comparatively more in all the dietary matrices. The concentrations of these elements are translated into intake rates through ingestion pathways. Daily intake (milligrams/day) of Fe, Cu, Ni, and Zn by adult population of Kakrapar, Gujarat were 16.5 +/- 6.2, 3.3 +/- 1.2, 1.8 +/- 1.0, and 3.6 +/- 1.3, respectively. Dietary sources of Fe, Cu, and Ni by Kakrapar adult population are comparable with RDA. In case of Zn, the daily dietary intake is comparatively lower than that of RDA.

Practice patterns of neurology in India: fewer hands, more work.

BACKGROUND: India is a populous country housing over a billion people. Neurology as a specialty is being practiced in India for over 50 years but the number of physicians devoted to fulltime neurology is limited. This fact coupled with the privatized healthcare system and limited infrastructure has led to situations different from the more developed healthcare systems. AIM: To study the practice patterns of neurology in India. SETTING AND DESIGN: Questionnaire-based study. MATERIALS AND METHODS: Questionnaire was sent to 250 members of the Indian Academy of Neurology [sample size approximately 25%] using random number table. The responses were tabulated and analyzed. RESULTS AND CONCLUSIONS: The neurology group is small and hence is exposed to a large workload. The average number of patients seen daily by Indian neurologists is three to four times those seen by the United States and United Kingdom neurologists. Neurologists based at district places are more likely to see direct patients; whereas metropolitan neurologists see more referrals. Investigative facilities are available to neurologists but affordability is a concern. Clinical work leaves less time for academic and research activities, which the consultants are keen to participate in. In the privatized health system of India, emergency work constitutes a difficult area to cope with. The concept of single specialty group practice is welcomed by the majority with the idea of streamlining their work and life. These factors highlight an urgent need for increasing the neurology work force and argue for further involvement of primary physicians and internists in neurological care in India.

Melanosomal proteins promote melanin polymerization.

In melanocytes, enzymes involved in the generation of melanin monomers are present and active in coated vesicles which are known to be acidic. Melanin polymerization however, occurs only in melanosomes. In vitro, it is not possible to generate melanin at the acidic pH of melanosomes using 3,4-dihydroxyphenylalanine (DOPA) and tyrosinase alone whereas melanin readily forms at higher pH with these reagents. Dimerization and elongation of the melanin polymer is known to require deprotonation. We have hypothesized that the amino acid side chains of melanosomal proteins act as proton acceptors to initiate polymerization and that the protonated basic groups serve to attract the negatively charged oligomers thus aiding polymerization and binding to proteins. We show that basic model proteins and basic premelanosomal proteins promote polymerization at an acidic pH and that positively charged surfaces allow binding of the growing melanin polymer. With progressive polymerization and exhaustion of the proton abstracting ability of melanosomal proteins, melanosomal pH drops further, which, we argue, is an additional controlling step that limits tyrosinase activity and melanin polymerization.

Donor corneal tissue evaluation.

Proper evaluation of donor cornea is critical to the success of corneal transplantation. Attention must be paid to the cause of death and ocular condition as several general and ocular diseases constitute contraindications for donor corneal usage. Death to enucleation time should be noted. Gross examination and slit lamp biomicroscopy are mandatory for the evaluation of the donor eye while specular microscopy adds another useful dimension to information regarding donor cornea. This article provides a comprehensive review of all the aspects of donor corneal evaluation as practised today worldwide.

Peripheral T lymphocytes and their subsets in cutaneous tuberculosis.

Fifty-four patients with cutaneous tuberculosis, consisting of 23 with lupus vulgaris, 22 with scrofuloderma, and nine with verrucosa cutis, were investigated for cell-mediated immunity, through estimation of peripheral total T lymphocytes (CD3+), CD4+ (helper/inducer), and CD8+ (cytotoxic/suppressor) lymphocytes, by immunohistochemical staining of peripheral blood smears, using specific monoclonal antibodies and the alkaline phosphatase-antialkaline phosphatase (APAAP) method. Absolute values of total T lymphocytes (CD3+), and CD4+ and CD8+ subsets, were found to be significantly raised in scrofuloderma, but the percentage values and the CD4+/CD8+ ratio remained unaltered. In tuberculosis verrucosa cutis, only the percentage of the CD8+ subset of T lymphocytes was found to be significantly lowered, and this altered the CD4+/CD8+ ratio. No significant change was observed in the peripheral blood T cells and their subpopulations in patients suffering from lupus vulgaris.

Evidence for the incorporation of [32P]orthophosphate into leaf inositol phospholipids.

Leaf discs of brinjal, tomato, sugar cane and maize rapidly incorporated [32P]orthophosphate into total phospholipids. Analyses of the labelled lipid extracts by thin-layer chromatography, autoradiography and comparison with inositol phospholipid standards demonstrated the labelling of phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate in addition to other phospholipids. The presence of polyphosphoinositides was further confirmed by deacylation of phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate and separation of the water-soluble products, glycerophosphoinositol phosphate and glycerophosphoinositol bisphosphate by formate exchange chromatography. Incorporation of [32P]orthophosphate into inositol phospholipids was time-dependent, with monoester phosphate groups attaining isotopic equilibrium within 90 min of incubation. After 2 h, incorporation of label into phosphatidylinositol, phosphatidylinositol monophosphate and phosphatidylinositol bisphosphate was about 15, 10 and 3%, respectively, of the total phospholipids. The ratio of radioactivity in phosphatidylinositol/phosphatidylinositol monophosphate/phosphatidylinositol bisphosphate was about 5:5:1 in brinjal leaves. However, this ratio may be an overestimate of the amounts of inositol phospholipids present, as other lysophospholipids may comigrate with standards.