Effects of glyphosate-based herbicide on gametes fertilization and four developmental stages in Clarias gariepinus.

Comparative toxicology continues to provide information on how the age of every living organism affects the frequency, severity, and nature of the potentially toxic agent. We investigated the effect of glyphosate-based herbicide (GBH) exposure on gametes and four developmental stages of Clarius gariepinus (C. gariepinus) (African Catfish). Gametes from healthy gravid female and mature male C. gariepinus were exposed to GBH in sublethal concentrations of 0.0 (G1, control), 0.02 (G2), 0.05 (G3), 0.1 (G4), 0.5 (G5), and 1.0 (G6) mg/L for 24 h at the standard conditions of temperature and water quality parameters. The surviving embryos were examined microscopically for malformation rate and edema occurrence post-GBH exposure. In a separate experiment; postfryer, fingerling, posfingerling and juvenile C. gariepinus were exposed to G1, G2, G3, G4, G5 and G6 of GBH concentrations daily consecutively for 28 days. Fish growth performance, behavioural changes, haematology, oxidative stress, and histology were assessed. From our results, GBH showed altered morphology 24 h post-fertilization, decreased body weight, growth parameters, behavioural indices, and survival rate in the various developmental stages. Oxidative stress metabolite, malondialdehyde levels, increases in the postfryer > postfingerlin > fingerling > juvenile C. gariepinus following GBH exposure. Leukopenia and thrombocytosis were observed in the postfingerlings and juvenile fish and decrease in the levels of reduced glutathione and activity of superoxide dismutase compared with the control. Histology showed gross necrosis of the fish gills, liver, brain, and cardiac myocytes in the exposed fish. Hence, our findings provide an insight into C. gariepinus developmental toxicity due to GBH, although continuous measurement of glyphosate levels in the fish and fish environment is essential.

Integrated community case management: planning for sustainability in five African countries.

BACKGROUND: The World Health Organization (WHO) launched an initiative to plan for the sustainability of integrated community case management (iCCM) programmes supported by the Rapid Access Expansion (RAcE) Programme in five African countries in 2016. WHO contracted experts to facilitate sustainability planning among Ministries of Health, WHO, nongovernmental organisation grantees, and other stakeholders. METHODS: We designed an iterative and unique process for each RAcE project area which involved creating a sustainability framework to guide planning; convening meetings to identify and prioritise elements of the framework; forming technical working groups to build country ownership; and, ultimately, creating roadmaps to guide efforts to fully transfer ownership of the iCCM programmes to host countries. For this analysis, we compared priorities identified in roadmaps across RAcE project sites, examined progress against roadmaps via transition plans, and produced recommendations for short-term actions based on roadmap priorities that were unaddressed or needed further attention. RESULTS: This article describes the sustainability planning process, roadmap priorities, progress against roadmaps, and recommendations made for each project area. We found a few patterns among the prioritised roadmap elements. Overall, every project area identified priorities related to policy and coordination of external stakeholders including funders; supply chain management; service delivery and referral system; and communication and social mobilisation, indicating that these factors have persisted despite iCCM programme maturity, and are also of concern to new programmes. We also found that a facilitated process to identify and document programme priorities in roadmaps, along with deliberately planning for transition from an external implementer to a national system could support the sustainability of iCCM programmes by facilitating teams of stakeholders to accomplish explicit tasks related to transitioning the programme. CONCLUSIONS: Certain common elements are of concern for sustaining iCCM programmes across countries, among them political leadership, supply chain management, data processes, human resources, and community engagement. Adapting and using a sustainability planning approach created an inclusive and comprehensive dialogue about systemic factors that influence the sustainability of iCCM services and facilitated changes to health systems in each country.

Heavy Metal Dietary Intake and Potential Health Risks for University Hostel Students.

The study was conducted to determine the residual concentration of Cd, Pb, Asand Hg in some foodstuffs served to university students living at hostels of Beni-Suef University (BSU), Egypt, and to estimate the dietary intake of such metals, as well as to assess the potential health risks associated with the consumption of such foods. Therefore, a total of 200 samples, 50 each of, soft cheese, UHT milk, raw broiler carcasses and canned tuna, were periodically collected from university hostels of BSU and examined for the residual levels of these metals using atomic absorption spectrophotometer. The obtained results revealed that the mean residual levels of Cd in soft cheese, UHT milk, broiler's meat and canned tuna samples were 0.37, 0.26, 0.089 and 0.093 mg/kg, respectively, while those of Pb were 0.187, 0.20, 0.181 and 0.164 mg/kg, respectively. Regarding As, they were 0.196, 0.24, 0.14 and 0.201 mg/kg, respectively, and Hg mean residual concentration accounted for 0.05, 0.05, 0.117 and 0.235 mg/kg, respectively. Some of the examined food samples had heavy metals' concentrations above the international standards. The total weekly dietary intakes of Cd, Pb, As and Hg were 4.99, 5.38, 4.77 and 2.76 µg/kg bw/week, respectively, that were comparable to the provisional tolerable weekly intake recommended by the Joint Expert Committee on Food Additives (JECFA). The total target hazard quotient (TTHQ) of broiler's meat was over 1 (1.686), thus indicating possible health risks in contrast to the TTHQs of other foodstuffs that were below one.

Heavy metal residues in beef carcasses in Beni-Suef abattoir, Egypt.

A total of 300 samples were collected from cattle slaughtered in the Beni-Suef abattoir in Egypt. Samples included muscle, liver and kidney. Animals were randomly selected from the slaughter line. The age of the slaughtered cattle was less than three years (18-30 months). Samples were packed separately in plastic bags, identified and stored at -18°C until analysis which was performed at the Max Rubner Institute in Kulmbach, Germany, for the following heavy metals residues: lead, cadmium, mercury, arsenic, chromium and nickel in beef muscle, liver and kidney samples. The results revealed that the overall mean residual levels of lead were 8.77 µg/kg, 42.70 µg/kg and 109.42 µg/kg fresh weight in muscle, liver and kidney samples, respectively, while the mean residual levels of cadmium were 1.40 µg/kg, 14.16 µg/kg and 62.56 µg/kg fresh weight, respectively, and the mean arsenic residual levels were 5.06 µg/kg, 4.64 µg/kg and 14.92 µg/kg fresh weight, respectively. The mean residual levels of mercury were 3.91 µg/kg, 5.81 µg/kg and 10.14 µg/kg fresh weight, respectively, and the residual levels of chromium were 11.20 µg/kg, 21.85 µg/kg and 25.49 µg/kg fresh weight, respectively. Finally, the mean residual levels of nickel were 21.17 µg/kg, 14.59 µg/kg and 34.95 µg/kg fresh weight, respectively. The mean values of all heavy metals examined were low and did not exceed the permissible limits adopted by different organisations. Most heavy metals accumulated in higher concentrations in the kidney in comparison to the liver and muscle.