RESUMO
Genotyping graft livers by short tandem repeats after human living-donor liver transplantation (n = 20) revealed the presence of recipient or chimeric genotype cases in hepatocytes (6 of 17, 35.3%), sinusoidal cells (18 of 18, 100%), cholangiocytes (15 of 17, 88.2%) and cells in the periportal areas (7 of 8, 87.5%), suggesting extrahepatic cell involvement in liver regeneration. Regarding extrahepatic origin, bone marrow mesenchymal stem cells (BM-MSCs) have been suggested to contribute to liver regeneration but compose a heterogeneous population. We focused on a more specific subpopulation (1-2% of BM-MSCs), called multilineage-differentiating stress-enduring (Muse) cells, for their ability to differentiate into liver-lineage cells and repair tissue. We generated a physical partial hepatectomy model in immunodeficient mice and injected green fluorescent protein (GFP)-labeled human BM-MSC Muse cells intravenously (n = 20). Immunohistochemistry, fluorescence in situ hybridization and species-specific polymerase chain reaction revealed that they integrated into regenerating areas and expressed liver progenitor markers during the early phase and then differentiated spontaneously into major liver components, including hepatocytes (≈74.3% of GFP-positive integrated Muse cells), cholangiocytes (≈17.7%), sinusoidal endothelial cells (≈2.0%), and Kupffer cells (≈6.0%). In contrast, the remaining cells in the BM-MSCs were not detected in the liver for up to 4 weeks. These results suggest that Muse cells are the predominant population of BM-MSCs that are capable of replacing major liver components during liver regeneration.
Assuntos
Transplante de Medula Óssea , Hepatopatias/cirurgia , Regeneração Hepática/fisiologia , Transplante de Células-Tronco Mesenquimais , Complicações Pós-Operatórias/terapia , Adulto , Animais , Criança , Feminino , Humanos , Técnicas Imunoenzimáticas , Hibridização in Situ Fluorescente , Transplante de Fígado/efeitos adversos , Masculino , Camundongos , Camundongos Endogâmicos ICR , Camundongos SCID , PrognósticoRESUMO
New dammarane-type triterpene oligoglycosides, jujubosides A1 and C and acetyljujuboside B1 were isolated from Zizyphi Spinosi Semen, the seeds of Zizyphus jujuba MILL. var. spinosa Hu, together-with three known saponins. The structures of jujubosides A1 and C and acetyljujuboside B were determined on the basis of chemical and physicochemical evidence. Jujubosides A1 and C and acetyljujuboside B were found to inhibit the histamine release from rat peritoneal exudate cells induced by antigen-antibody-reaction.
Assuntos
Glicosídeos/síntese química , Glicosídeos/farmacologia , Antagonistas dos Receptores Histamínicos/química , Antagonistas dos Receptores Histamínicos/farmacologia , Extratos Vegetais/farmacologia , Plantas Medicinais/química , Saponinas/química , Saponinas/farmacologia , Animais , Líquido Ascítico/citologia , Sequência de Carboidratos , Glicosídeos/isolamento & purificação , Liberação de Histamina/efeitos dos fármacos , Masculino , Conformação Molecular , Dados de Sequência Molecular , Extratos Vegetais/isolamento & purificação , Ratos , Ratos Wistar , Sementes/química , Relação Estrutura-AtividadeRESUMO
Several years ago, we reported that a Sendai virus (SeV) defective genome (DIH4UV) could be rescued in vivo with human parainfluenza virus type 1 (hPIV1) and bovine PIV3 but not by measles virus or vesicular stomatitis virus. It was concluded that the cis-acting RNA sequences were conserved within the SeV/PIV1/PIV3 group but that interactions between the polymerase complex (P-L) and the template protein N were unique for each virus. We have re-examined these conclusions using proteins expressed from cloned N, P and L genes for SeV and PIV3. The results demonstrate the specificity of the protein-protein interactions between polymerase and template, and confirm the prediction of the earlier work that PIV3 N, P and L proteins are capable of assembling and replicating SeV mini-genomes also expressed from a cDNA clone.
Assuntos
DNA Viral , Nucleocapsídeo/genética , Fosfoproteínas/genética , Respirovirus/genética , Moldes Genéticos , Proteínas Virais/genética , Animais , Bovinos , Linhagem Celular , Cricetinae , DNA Complementar , Humanos , RNA Viral , Células Tumorais CultivadasRESUMO
By determining gene nucleotide sequences we compared the primary structures of the membrane (M), fusion (F), and hemagglutinin-neuraminidase (HN) proteins of bovine parainfluenza 3 virus strains, M, SC, and MR which are substrains derived from a wild strain YN. The M and SC viruses are indistinguishable in having very weak hemagglutination (HA) and neuraminidase (NA) activities, but M virus' syncytium-inducing (SI) activity is considerably higher than that of the SC virus. However, the results showed that the amino acid sequence of the F protein was identical in M and SC viruses, demonstrating that M virus' high SI activity was not due to alteration of its F protein. Two differences in M and SC viruses' other proteins then seemed to be important, although their significance in the SI activity is not clear at present; the first being the 70th amino acid residue of the M protein, which was Asp in the M virus and Gly in the SC virus, and the other being the 539th residue of the HN protein, which was Tyr in the M virus and His in the SC virus. The nucleocapsid proteins of both M and SC viruses were identical. The MR virus, which is a variant derived from the M virus and has high HA and NA activities but very weak SI activity, was different from the M virus at only one site throughout the M, F, and HN proteins; the 193rd amino acid residue of the HN protein was Leu in the MR virus and Phe in the M virus. This result strongly suggested that the substitution of Leu with Phe at this particular site was closely linked to the drastic reduction in both HA and NA activities.
