Neutrophil dysfunction in rats with natural gingivitis.

The functions of polymorphonuclear neutrophils (PMN) from SUS rats with naturally occurring gingivitis were examined by the luminol-dependent chemiluminescence (CL), adherence and bactericidal tests. SUS rats with pre-gingivitis showed lower CL responses of isolated PMNs and whole blood than control rats (RES rats). After plague formation and progression of gingivitis, the CL response gradually increased in SUS rats. RES rats had healthy gingiva and showed no increase in CL responses. Impaired PMN adherence was observed in SUS rats with pre-gingivitis but not in RES rats. PMNs from SUS rats with pre-gingivitis also showed lower bactericidal activity than those from RES rats. Dysfunction of PMNs might induce gingivitis as a result of decreased protection against periodontal pathogens and an elevated level of CL response can be recognized with progression of gingivitis.

Application of a sucrose indicator strip to evaluate salivary sucrose clearance.

This study examined salivary sucrose clearance times of 96 subjects using sucrose indicator strips. Subjects with high caries experience had a longer salivary sucrose clearance time than those with a low caries experience. The flow rate of unstimulated but not of stimulated saliva was significantly related to sucrose clearance. This finding is consistent with that of other studies on salivary clearance. The sucrose indicator strips are simple to use and sucrose clearance tests using these strips do involve instrumental analyses. It is concluded that the strips investigated are simple and effective and merit further investigation.

[Dental caries of primary teeth and life habits in Shinshinotsu Nursery School: three years of observations].

It is well known that there is a high positive correlation between increases in caries increment and life habits such as use of nursing bottle, tooth brushing, between-meal eating, and sweet consumption. The purpose of this study was to analyze data of caries status in primary dentition and life habits observed in 373 Shinshinotsu nursery schoolchildren aged 3-5 years over three years. Dental examinations were conducted in good natural light using dental mirror and probe. Caries was diagnosed according to the Welfare Ministry criteria and life habits were examined by a questionnaire. The mean number of df was 7.1 for the whole sample for three years and 17.6 of the children were caries free, whereas 47.3 were more than 10 df. The former group showed the best score for the frequency of between-meal eating, frequency of sweet consumption, tooth brushing habits, and frequency of fluoride application. The latter group showed a tendency to lower sweet consumption during the three years. Our data also showed that the life habits at nursing (use of nursing bottle and weaning period) influenced incidence of caries.

Chemiluminescence and phagocytic responses of rat polymorphonuclear neutrophils to leptospires.

The interaction of leptospires with polymorphonuclear neutrophils (PMN) was examined by the luminol-dependent chemiluminescence (CL) test. Whole blood CL changed in relation to the stage of leptospiral infection both in susceptible (SUS) and resistant (RES) rats. The intensity of CL grew with an increasing number of leptospires in the blood. CL responses were observed in isolated PMN upon exposure to living leptospires. In contrast, the same bacteria, having been inactivated by formalin, did not stimulate PMN. A variation was found in the CL response by different living strains of Leptospira. The CL intensity was arranged as follows: L. illini greater than L. biflexa greater than L. interrogans avirulent strains greater than L. interrogans virulent strains. The CL response was markedly enhanced by an opsonization of leptospires. Specific opsonization was shown to increase the rate of phagocytosis of leptospires with relation to the CL response.

[Dental caries of deciduous teeth in nursery school children in Shinshinotsu: changes in prevalence data for three years from 1986 to 1988].

The purpose of this study was to survey the changes in dental caries prevalence in 3-, 4-, and 5-year-old nursery school children in Shinshinotsu for 3 years from 1986 to 1988. The results obtained were as follows: 1) The caries prevalence in 5-year-old Shinshinotsu children decreased from 1986, and was 87.5% in 1988, lower than the national survey in 1987. On the other hand, the number of df teeth per person and the incidence of type C caries increased in 5-year-old Shinshinotsu children. 2) The caries prevalence rate in 3-year-old Shinshinotsu children was 89.5% in 1988, rather higher than the national survey in 1987.