Changes in insulin-like growth factor I, 17-b-estradiol, and progesterone in postmenopausal women with benign and malignant ovarian tumours.

BACKGROUND: The aim of this research was to observe changes in IGF-I and steroid hormones in postmenopausal women with benign and malignant ovarian tumors. MATERIAL AND METHODS: The concentrations of insulin-like growth factor I (IGF-I), 17-b-estradiol and progesterone were examined by radioimmunological assay in three groups of postmenopausal women: patients with malignant ovarian tumors, patients with benign ovarian tumors, and healthy women. In all three groups the age and body mass index (BMI) were comparable. RESULTS: The concentrations of IGF-I in the serum of the patients with malignant cancer were significantly lower. The concentrations of estradiol were significantly higher in the patients with both benign and malignant ovarian tumors as compared to the controls. CONCLUSIONS: Decreased IGF-I concentration in the serum of postmenopausal women with ovarian tumors may suggest that the tumor is malignant.

Function of insulin-like growth factor (IGF-I) and its binding protein (IGFBP-1) in pathological proliferation of endometrium.

The purpose of this project was the investigation of insulin, IGF-I and IGFBP-1 concentrations in the serum of patients with pathological changes in endometrium. IGF-I and its binding protein (IGFBP-1) stimulate cell growth and differentiation of the normal and neoplastic endometrium. The mean values of IGF-I concentrations in the group of patients with atypical hyperplasia and endometrial carcinoma were significantly higher than in the control group with normal endometrium. Significantly higher IGFBP-1 concentrations were found in the serum of patients with the atypical hyperplasia of endometrium and endometrial carcinoma. The correlation between insulin concentration and changes in endometrium in the examined groups was not found.

[The influence of demographic and behavioral factors on the risk of human papillomavirus infection]. / Ocena wplywu wybranych czynników demograficznych i srodowiskowych na ryzyko wystapienia infekcji wirusami brodawczaka ludzkiego.

OBJECTIVES: The aim of this work was the assessment of correlation between different types of HPV and chosen factors: the number of pregnancies, childbirths and smoking habits. MATERIALS AND METHODS: Patients were examined in the Gynaecology Clinic, Silesian Medical Academy in Zabrze from 1998 until 2000. They were either hospital patients or women subjected to routine cytological examination. The smears were simultaneously collected for both cytooncologic examination and PCR identification of HPV viruses. Cytological smears were classified according to the Bethesda system. RESULTS: A relationship between the presence of HPV and number of pregnancies or childbirths was confirmed as well as the relationship between smoking and the presence of HPV genome. The higher number of pregnancies or childbirths the higher frequency of HPV presence is. HPV presence was more frequent in the group of smoking women. CONCLUSIONS: A relationship between the presence of HPV and the number of past childbirth or pregnancies was found. The statistically significant correlation between smoking and the risk of HPV infection was also confirmed.

[Chromosome instability in women with genital organs carcinoma]. / Niestabilnosc chromosomowa kobiet z choroba nowotworowa narzadu rodnego.

OBJECTIVES: The aim of the current work was the assessment of the mutagen susceptibility of chromosomes of patients with carcinomas in comparison to healthy volunteers. It was interesting whether the bleomycin assay can be useful for searching for more susceptible to cancer disease individuals. MATERIALS AND METHODS: There were 4 groups of patients analysed: controls and three test groups (patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma). In total 108 female patients were examined by use of bleomycin assay. Lymphocytes were cultured in vitro and treated with bleomycin. The b/c (breaks per cell) index was evaluated by use of light microscopy. RESULTS: Statistically significant increased test values were found in patients with uterine cervix carcinoma, endometrium carcinoma and ovarian carcinoma. CONCLUSIONS: The assessment of chromosome instability could be a useful prognostic test in the diagnosis of carcinoma of female genital organs. The bleomycin assay is useful for searching subpopulations with higher chromosome instability and more susceptible to cancer disease.

[Detection of human papillomavirus genome in cervical squamous intraepithelial lesions]. / Badania nad obecnoscia genomu onkogennych typów wirusa brodawczaka ludzkiego w wybranych stanach patologii szyjki macicy.

OBJECTIVES: The aim of this work was the assessment of the correlation between different types of HPV and abnormal stages of uterine cervix. MATERIALS AND METHODS: The smears were simultaneously collected for both cytooncologic examination and PCR identification of HPV viruses. Cytologic smears were classified according to the Bethesda system. RESULTS: A strong relationship between the presence of HPV types 16, 18 and 33 and the intensification of cytologic changes was confirmed. The more advanced abnormal changes in the uterine cervix, the more often presence of these HPV types. The presence of HPV types 6 and 11 were more often in low grade lesions. CONCLUSIONS: The conclusions from this examinations are: there is a proportional correlation between the grades of Squamous Intraepithelial Lesions, uterine cervix carcinoma and the presence of oncogenic types of human papillomavirus.

[Laparotomy performed in pregnant women due to non-obstetrical causes]. / Laparotomie wykonane u ciezarnych z przyczyn niepolozniczych.

OBJECTIVE: The clinical characteristics of laparotomy during pregnancy were investigated. MATERIALS AND METHODS: A series of 24 cases of laparotomy during pregnancy was investigated with respect to clinicopathologic features and outcome. RESULTS: Among 23 cases there were 20 ovarian tumors, 2 acute appendicitis and 2 uterine fibroid. Gestational ages at the time of surgery ranged from 16 to 24 weeks of patients with ovarian tumors and from 30 to 32 weeks in others. No intraoperative or postoperative maternal or fetal complications occurred. One patient had a stage III ovarian carcinoma and went to total hysterectomy, and other patient had a stage IA ovarian carcinoma. CONCLUSIONS: Appendectomy can be performed without additional risk to the fetus for those who require surgical intervention during pregnancy. Ovarian surgery in pregnancy for persistent masses is important to obtain a final histologic diagnosis.

[Carrier of numerical chromosome abnormalities in infertile married couples]. / Nosicielstwo anomalii liczbowych chromosomowych wsród malzenstw z niepowodzeniami prokreacji.

OBJECTIVES: Genetic anomalies are one of many conditions causing infertility. DESIGN: The aim of the study was to define the frequency of numerical chromosome aberrations in infertile married couples. MATERIALS AND METHODS: Metaphasal chromosomes were analysed by G-T-G stration in 650 patients, i.e. 325 married couples. Chromosomes for testing had been obtained from peripheral blood lymphocytes. RESULTS: Of all women and men, numerical chromosome anomalies were found in 14 married couples (4.1%). The aberrations were connected with female factors in 8 couples (2.3%), and with male factors in 6 (1.8%). CONCLUSIONS: Results of the study indicate that diagnostic procedure for infertility should preferably include cytogenetic examinations as well.

[Computer analysis of a cardiotocogram and parameters of acid-base balance in capillary blood of the infant]. / Komputerowa analiza kardiotokogramu a parametry równowagi kwasowo-zasadowej krwi wlosniczkowej noworodka.

Our objective in this study was to compare the parameters of the acid-base balance in the capillary blood 60 minutes after delivery with the antenatal cardiotocographic computer analysis. Our study included 64 infants delivered vaginally on term. The basic criterion to classify into the examined group and control group was a pH result of the capillary blood equal to 7.30. In the examined group there were 34 cases and 30 in the control group. Retrospective analysis of cardiotocographs was carried out. The determination was made of pH, pCO2, pO2, HCO3, SBE and SAT in the capillary blood. The analysed material showed a very good negative correlation between a pH value in the examined group and a short-term variability (STV) (r = 0.95 at p < 0.001). It was concluded that there is a strong relationship between elements of computer-aided cardiotocographic monitoring and abnormalities in the acid-base balance and condition of the infant.