RESUMO
The acceptance of camphor tree (Cinnamomum camphora) as a host plant for the larvae of common bluebottle (Graphium sarpedon nipponum) was explained by the presence of feeding stimulants in the leaves. When the active methanol extract of C. camphora leaves was separated into hexane and water layers, both layers showed high feeding activities for the larvae of G. sarpedon nipponum. Bioassay-guided fractionation of the hexane layer resulted in the isolation of a highly active compound, which was identified as a-linolenic acid by nuclear magnetic resonance spectrometry and gas chromatography-mass spectrometry.
Assuntos
Estimulantes do Apetite/farmacologia , Apetite/fisiologia , Cinnamomum camphora/química , Lepidópteros/efeitos dos fármacos , Extratos Vegetais/farmacologia , Folhas de Planta/química , Ração Animal , Animais , Apetite/efeitos dos fármacos , Estimulantes do Apetite/isolamento & purificação , Ovos , Comportamento Alimentar/efeitos dos fármacos , Comportamento Alimentar/fisiologia , Feminino , Hexanos , Larva/efeitos dos fármacos , Larva/fisiologia , Extratos Vegetais/análise , Água , Ácido alfa-Linolênico/isolamento & purificação , Ácido alfa-Linolênico/farmacologia , Ácido gama-Linolênico/isolamento & purificação , Ácido gama-Linolênico/farmacologiaRESUMO
Floral scent has been shown to elicit behavioral responses by butterflies which forage for flowers after receiving appropriate signals. In comparison with investigations about the role of floral scent, those of foliar odor are, however, very few. In this study, the foliar volatiles of Cinnamomum camphora (Lauraceae), which had been collected by air entrainment, exhibited activities toward Graphium sarpedon nipponum (Papilionidae) in both electrophysiological and behavioral tests. The volatiles were analyzed by capillary gas chromatography with electro-antennographic detection (GC-EAD). Two electrophysiological active compounds were found which were determined as nonanal and decanal by using gas chromatography-mass spectrometry (GC-MS). Female butterflies generally tend to show a greater EAG response than males to the headspace volatiles and EAG-active aldehydes. Two EAG-active aldehydes were found in attractant tests to be attractive to both sexes of the butterfly when treated individually. Although the difference between the sexes was not significant, the female butterflies' preference tended to be more active than that of the males.