Carbon Sequestration in Resin-Tapped Slash Pine (Pinus elliottii Engelm.) Subtropical Plantations.

Every year more than 150,000 tons of resin used in a myriad of industrial applications are produced by Brazilian plantations of Pinus elliottii Engelm. (slash pine), which are also used for timber. A pine tree can be tapped for resin over a period of several years. Resin is a complex mixture of terpenes, which are carbon-rich molecules, presumably influencing pine plantation carbon budgets. A total of 270 trees (overall mean DBH of 22.93 ± 0.11 cm) of 14-, 24-, and 26-year-old stands had their C content measured. Three different treatments (intact, wounded panels, and wounded + chemically stimulated panels, 30 trees each) were applied per site. Above- and belowground biomass, as well as resin yield, were quantified for two consecutive years. Data were statistically evaluated using normality distribution tests, analyses of variance, and mean comparison tests (p ≤ 0.05). The highest resin production per tree was recorded in the chemically stimulated 14-year-old stand. Tree dry wood biomass, a major stock of carbon retained in cell wall polysaccharides, ranged from 245.69 ± 11.73 to 349.99 ± 16.73 kg among the plantations. Variations in carbon concentration ranged from 43% to 50% with the lowest percentages in underground biomass. There was no significant difference in lignin concentrations. Soils were acidic (pH 4.3 ± 0.10-5.83 ± 0.06) with low C (from 0.05% to 1.4%). Significantly higher C stock values were recorded in pine biomass compared to those reported for temperate zones. Resin-tapping biomass yielded considerable annual increments in C stocks and should be included as a relevant component in C sequestration assessments of planted pine forests.

A comprehensive and critical review on key elements to implement enzymatic PET depolymerization for recycling purposes.

Plastics production and recycling chains must be refitted to a circular economy. Poly(ethylene terephthalate) (PET) is especially suitable for recycling because of its hydrolysable ester bonds and high environmental impact due to employment in single-use packaging, so that recycling processes utilizing enzymes are a promising biotechnological route to monomer recovery. However, enzymatic PET depolymerization still faces challenges to become a competitive route at an industrial level. In this review, PET characteristics as a substrate for enzymes are discussed, as well as the analytical methods used to evaluate the reaction progress. A comprehensive view on the biocatalysts used is discussed. Subsequently, different strategies pursued to improve enzymatic PET depolymerization are presented, including enzyme modification through mutagenesis, utilization of multiple enzymes, improvement of the interaction between enzymes and the hydrophobic surface of PET, and various reaction conditions (e.g., particle size, reaction medium, agitation, and additives). All scientific developments regarding these different aspects of PET depolymerization are crucial to offer a scalable and competitive technology. However, they must be integrated into global processes from upstream to downstream, discussed here at the final sections, which must be evaluated for their economic feasibility and life cycle assessment to check if PET recycling chains can be broadly incorporated into the future circular economy.

Iron biofortification in rice: it's a long way to the top.

Rice and most staple cereals contain low iron (Fe) levels, most of which is lost during grain processing. Populations with monotonous diets consisting mainly of cereals are especially prone to Fe deficiency, which affects about two billion people. Supplementation or food fortification programs have not always been successful. Crop Fe fertilization is also not very effective due to Fe soil insolubility. An alternative solution is Fe biofortification by generating cultivars that efficiently mobilize, uptake and translocate Fe to the edible parts. Here, we review the strategies used for the Fe biofortification of rice, including conventional breeding and directed genetic modification, which offer the most rapid way to develop Fe-rich rice plants. While classical breeding is able to modify the contents of inhibitors of Fe absorption, transgenic approaches have focused on enhanced Fe uptake from soil, xylem and phloem loading and grain sink strength. A comprehensive table is provided in which the percentages of the recommended dietary Fe intake reached by independently developed transgenic plants are calculated. In this review we also emphasize that the discovery of new QTLs and genes related to Fe biofortification is extremely important, but interdisciplinary research is needed for future success in this area.