RESUMO
Total IgG, IgM and IgA levels and toxoplasma IgG, IgM and IgA immunoblotting patterns were assayed in 10 sera before and after IgG absorption with Protein G-Sepharose 4. Removal of IgG (mean reduction 96%) was accompanied by a significant reduction in the level of IgM (mean reduction 56%) and IgA (mean reduction 53%) in nine of the 10 sera. The absorbed supernates showed fewer and weaker IgM bands in five sera, but IgA immunoblotting patterns were unaffected by absorption. There was no benefit in removing IgG in toxoplasma IgM and IgA immunoblotting.
Assuntos
Anticorpos Antiprotozoários/análise , Imunoglobulina A/análise , Imunoglobulina G/metabolismo , Imunoglobulina M/análise , Toxoplasma/imunologia , Absorção , Animais , Anticorpos Antiprotozoários/metabolismo , Humanos , Immunoblotting , Imunoglobulina G/análise , Proteínas do Tecido Nervoso/metabolismo , Sefarose/metabolismoRESUMO
Sera from 591 patients were examined for total IgE concentration and for reactivity against a panel of seven specific allergens, using the radio-allergosorbent test (RAST). As the total IgE increased, the frequency of positive RAST reactions increased (P less than 0.001); there were more multiple positive RAST reactions (P less than 0.001); and there was a higher grade of RAST reaction (P less than 0.001). However, 12% of patients with normal IgE levels were positive by RAST. From these data a sequential protocol for testing sera of patients with suspected allergy has been developed. Sera should first be tested for total IgE, RAST reactivity to grass pollen and house dust mite. Samples reactive in either assay should be tested with the remaining five discs of the full screen. This protocol reduces unnecessary tests, but allows identification of sera with positive RAST results.
