Preparation method of polystyrene core-poly (acrylamide-acrylic acid) shell fluorescent microspheres / 浙江大学学报·医学版

<p><b>OBJECTIVE</b>To investigate the preparation method of polystyrene core-poly (acrylamide-acrylic acid) shell fluorescent microspheres.</p><p><b>METHODS</b>The polystyrene core-poly (acrylamide-acrylic acid) shell (P-(St-co-AAM)) fluorescent microspheres were prepared using fluorescent microspheres as the core and acrylamide/acrylic as polymerization monomer. Reaction conditions affecting the morphology of core-shell structure including feeding mode, initiator, cross linker, pH, concentration and swelling were studied.</p><p><b>RESULT</b>Fluorescent microscopy showed that the relatively uniform particle sizes were distributed in a range of 7-8 μm. Fourier transform infra-red spectroscopy (FT-IR) proved the existence of poly (acrylamide-acrylic acid) shell and amide group on the surface. The optimal conditions for seeding polymerization: azobisisobutyronitrile was used as the initiator in the absence of cross linker, after a 40 h swelling treatment by using alcohol with the appropriate reaction temperature (70 degree), reaction time (3 h) and pH(6-7). The average dispersion and stability were 25.14 % and 90.21%, respectively. The fluorescein release percentage was kept stable at approximately 30% after 40 h.</p><p><b>CONCLUSION</b>The fluorescent microspheres prepared by this method have core-shell structure and satisfactory fluorescence properties with good dispersion and stability.</p>

The personal experiences of HIV/AIDS patients in rural areas of western China.

Because of unique economic, cultural, and social factors, the personal experiences of HIV/AIDS patients in the rural areas of western China are not well understood. The aim of this study was to explore HIV/AIDS patients' thoughts and personal experiences in the environment and conditions of rural western China. Sixteen HIV/AIDS patients from remote western areas of China were interviewed by using an established qualitative method, during April to June 2008. The interviews were transcribed, and the findings were extracted after the interview content was read, classified, and analyzed repeatedly. We uncovered five general categories of personal experiences: worsening of family economic status, profound influence of traditional village culture, the family as the dynamic of surviving, an enhanced sense of familial responsibility, and dramatic psychological changes. The results of this study can provide useful information to assist health care professionals in China in their efforts to improve the general condition of these HIV/AIDS patients.

Analgesic effect of oxysophoridine and its mechanism / 中草药

Objective To investigate the analgesic effect of oxysophoridine(OSR)and the influence of verapamil(Ver)on the antinociception of OSR when two drugs were co-administrated in mice.Methods The number of writhing within 15 min after ip different doses of OSR was observed in painful mouse mo- dels caused by acetic acid.The hot plate method was used to assess nociceptive sensitivity of CaCl_2 and Ver before ip OSR.Nitric oxide(NO)in serum was measured by spectrophotometry.Results The number of writhing was decreased and the latency of licking the hind paws was prolonged in a dose-dependent manner after ip OSR.The antinociception of OSR could be antagonized by CaCl_2 and enhanced by Ver.No inter- ference was detected in serum volume of NO.Conclusion These results suggest that OSR can antagonize the acute pain caused by acetic acid and hot plate in a dose-dependent manner in mice.Calcium channel blocker could enhance the effect of OSR.