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1.
Artigo em Inglês | MEDLINE | ID: mdl-38862424

RESUMO

The order Acipenseriformes, which includes sturgeons and paddlefishes, represents "living fossils" with complex genomes that are good models for understanding whole-genome duplication (WGD) and ploidy evolution in fishes. Here, we sequenced and assembled the first high-quality chromosome-level genome for the complex octoploid Acipenser sinensis (Chinese sturgeon), a critically endangered species that also represents a poorly understood ploidy group in Acipenseriformes. Our results show that A. sinensis is a complex autooctoploid species containing four kinds of octovalents (8n), a hexavalent (6n), two tetravalents (4n), and a divalent (2n). An analysis taking into account delayed rediploidization reveals that the octoploid genome composition of Chinese sturgeon results from two rounds of homologous WGDs, and further provides insights into the timing of its ploidy evolution. This study provides the first octoploid genome resource of Acipenseriformes for understanding ploidy compositions and evolutionary trajectories of polyploid fishes.


Assuntos
Evolução Molecular , Peixes , Genoma , Poliploidia , Sequenciamento Completo do Genoma , Animais , Peixes/genética , Sequenciamento Completo do Genoma/métodos , Genoma/genética , Filogenia
2.
Sci Rep ; 14(1): 4231, 2024 02 20.
Artigo em Inglês | MEDLINE | ID: mdl-38378745

RESUMO

Chinese sturgeon Dmrt gene family was identified and characterized for the first time. A total of 5 putative Dmrt genes were identified. The gene structure, conserved protein domain and the phylogenetic relationship of Dmrt gene family were systematically analyzed. The expressed profile of Chinese sturgeon Dmrt genes in gonad, pituitary and hypothalamus in the male and female were investigated. The results indicated that the accumulation of Dmrt genes was involved in different tissues, and the expression profile also differed among each Dmrt genes. ASDmrt1A, ASDmrt2, ASDmrt3, and ASDmrtA1 were highly expressed in the testis in comparison with other tissue. This result showed that ASDmrt1A, ASDmrt2, ASDmrt3, and ASDmrtA1 played an important role in the development of testicle, and may be useful tool in distinguishing between male and female of Chinese sturgeon. Our study will provide a basis for additional analyses of Chinese sturgeon Dmrt genes. This systematic analysis provided a foundation for further functional characterization of Dmrt genes with an aim of study of Chinese sturgeon Dmrt gene family.


Assuntos
Peixes , Testículo , Animais , Masculino , Feminino , Filogenia , Peixes/genética , Peixes/metabolismo , Testículo/metabolismo , Gônadas , Genoma
4.
Sci Rep ; 10(1): 17399, 2020 10 15.
Artigo em Inglês | MEDLINE | ID: mdl-33060650

RESUMO

In this study, the effects of different feeding frequencies on the growth and the expression of genes in the GH/IGF axis were assessed in juvenile Chinese sturgeon. The newly hatched Chinese sturgeons were bred for 38 days at three different feeding frequencies groups (feeding frequency of two times a day, TWD; three times a day, THD; and four times a day, FOD), and the expression levels of the GH/IGF axis responses to feeding frequency were determined by quantitative real-time PCR. In addition, the full-length of the Coding Sequences of IGF I and IGF II genes (489-bp and 660-bp, respectively), were cloned and analyzed from Chinese sturgeon the first time. Multiple sequence alignments of IGFs revealed that Chinese sturgeon are high sequence identity to IGFs from other species. The phylogenetic relationships based on the IGF I and IGF II amino acid sequences were consistent with the traditional classification. After 38 days of growth, the three different feeding frequencies groups of Chinese sturgeon had no significant difference of body length, body weight, specific growth rate, the survival rate, the rate of weight gain and the condition factor. However, the relative expression of Chinese sturgeon GH in the pituitary decreased with increasing feeding frequency. The relative expression of Chinese sturgeon GHR in liver and skeletal muscle was deceased with increasing feeding frequency, while the relative expression of GHR in stomach and intestines at THD group was significantly higher than that of at TWD group and FOD group (p < 0.05). The relative expression of Chinese sturgeon IGF I in liver increased significantly with increasing feeding frequency (p < 0.05). The relative expression of IGF I in stomach and skeletal muscle was similar at the three groups. The relative expression of IGF I in intestines was significantly higher at FOD group than at TWD group and THD group (p < 0.05). The relative expression of Chinese sturgeon IGF II in liver at TWD group was significantly higher than that at THD group and FOD group (p < 0.05). However, the relative expression of IGF II in stomach, intestines and skeletal muscle at THD group was higher than that at TWD group and FOD group. Based on these previous studies that liver IGF I is regarded as a biomarker of growth performance, this result suggested that the juvenile Chinese sturgeon is better for growth when feeding four times daily compared to twice and thrice daily.


Assuntos
Comportamento Alimentar , Peixes/fisiologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Peixes/genética , Regulação da Expressão Gênica , Hormônio do Crescimento/genética , Fator de Crescimento Insulin-Like I/genética , Fator de Crescimento Insulin-Like II/genética , Filogenia , Reação em Cadeia da Polimerase , Alinhamento de Sequência
5.
In Vitro Cell Dev Biol Anim ; 56(8): 650-658, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32888116

RESUMO

Yangtze sturgeon (Acipenser dabryanus) is an endangered endemic freshwater fish of China. Cell-line is a potential means used for long-term preservation of germplasm resources and an ideal in vitro model in place of living organisms for biological studies. Here, culture condition and characterization of fin-derived cell in Yangtze sturgeon were carried out. Tissue explant techniques have been efficiently used in the Yangtze sturgeon caudal fin (YSCF) culture. The YSCF cell line showed a fibroblast-like morphology and stable growth in minimum essential medium eagle's (MEME) supplemented with 10-20% fetal bovine serum at 25°C. Cells were cryopreserved with preservative DMSO in liquid nitrogen and grew normally after recovery. No bacterial, fungal, or mycoplasma contamination was detected in the YSCF cells. Karyotype analysis of the YSCF cells showed that the chromosome numbers of the YSCF ranged from 242 to 273, and the modal chromosome number was identified as 264 at passage 9. The YSCF cells were confirmed from A. dabryanus by assay of 16S rRNA and COI. Furthermore, GFP reporter gene was successfully transferred into YSCF cells and expressed. The established YSCF cell lines will contribute to the preservation of germplasm resources and provide a useful vitro tool for further biological studies in sturgeon species.


Assuntos
Nadadeiras de Animais/citologia , Técnicas de Cultura de Células/métodos , Linhagem Celular/citologia , Espécies em Perigo de Extinção , Peixes/metabolismo , Animais , Sequência de Bases , Proliferação de Células , Forma Celular , Cromossomos , Criopreservação , Genes Reporter , Proteínas de Fluorescência Verde/metabolismo , Cariótipo , Metáfase , RNA Ribossômico 16S/genética
6.
Sci Rep ; 10(1): 3451, 2020 02 26.
Artigo em Inglês | MEDLINE | ID: mdl-32103060

RESUMO

Chinese sturgeon (Acipenser sinensis) has been listed as a critically endangered species on the IUCN Red List and is an endemic fish of China. Five sets of duplex polymerase chain reactions (PCR) assays were developed with 10 tetranucleotide microsatellites for Chinese sturgeon. The size of CS57, ZHX43, ZHX69, AS105, ZHX51, AS074, ZHX2, AS078, AS026 and AS073 products in 184 Chinese sturgeon individuals ranged from 257-305, 191-241, 251-285, 172-244, 236-260, 169-209, 194-234, 92-176, 165-257 and 120-164, respectively. The observed allele number of the 10 microsatellites ranged from 7 to 16, and the total number of alleles was 106. The number of alleles per individual in CS57, ZHX43, AS105, AS074, AS078 and AS026 was 1-4. The number of alleles per individual in ZHX69, ZHX51, ZHX2 and AS073 was 2-4. The mean number of alleles per locus per individual ranged from 2.01-3.76. The expected heterozygosity (HE), observed heterozygosity (HO), polymorphic information content (PIC) and Shannon-Weiner diversity index (H') ranged from 0.582 to 0.899, from 0.676 to 1, from 0.518 to 0.886 and from 1.034 to 2.34, respectively. Despite many advantages, the use of microsatellites as genetic analysis tools can be limited by the cost of the associated experiment. To solve this problem, this set of five duplex PCRs will provide tools that are more helpful, less expensive and less time consuming than others used for genetic analyses in Chinese sturgeon.


Assuntos
Peixes/genética , Variação Genética , Alelos , Animais , China , Espécies em Perigo de Extinção , Peixes/crescimento & desenvolvimento , Repetições de Microssatélites/genética , Reação em Cadeia da Polimerase/métodos , Transcriptoma
7.
Sci Data ; 6(1): 87, 2019 06 13.
Artigo em Inglês | MEDLINE | ID: mdl-31197171

RESUMO

Chinese sturgeon (Acipenser sinensis), a critically endangered Acipenseridae family member, is one of the largest anadromous, native fish in China. Numerous research programmes and protection agencies have focused on breeding and preserving this endangered species. However, available information is limited on the different stages of sex development, especially on the reproductive regulation of the hypothalamus-pituitary-gonad (HPG) axis of A. sinensis. To unravel the mechanism of gene interactions during sex differentiation and gonad development of A. sinensis, we performed transcriptome sequencing using HPG samples from male and female A. sinensis in two developmental stages. In this study, 271.19 Gb high-quality transcriptome data were obtained from 45 samples belonging to 15 individuals (six in stage I, six males and three females in stage II). These transcriptomic data will help us understand the reproductive regulation of the HPG axis in the development stages of A. sinensis and provide important reference data for genomic and genetic studies in A. sinensis and related species.


Assuntos
Espécies em Perigo de Extinção , Peixes/fisiologia , Perfilação da Expressão Gênica , Sistema Hipotálamo-Hipofisário/fisiologia , Diferenciação Sexual/genética , Animais , Feminino , Peixes/genética , Genoma , Masculino , Transcriptoma
8.
Gene ; 666: 1-8, 2018 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-29733966

RESUMO

The Chinese sturgeon (Acipenser sinensis) is an anadromous fish distributed in the Yangtze River and the East China Sea. In this study, we report the novel finding of cleft palates in Chinese sturgeons derived from artificial fertilization. To explore the genetic basis of palate malformation in A. sinensis, Illumina RNA-seq technology was used to analyze the transcriptome data of farmed Chinese sturgeons with normal palates and cleft-palates. Raw reads were obtained and assembled into 808,612 unigenes, with an average length of 509.33 bp and an N50 of 574 bp. Sequence similarity analyses against four public databases (Nr, UniProt, KEGG, and COGs) found 158,642 unigenes that could be annotated. GABAergic synapses and TGF-ß signal pathways were the two most enriched pathways with high Rich Factors in the analyses of differentially expressed genes. In these two signal pathways, six genes (GABRA4, GS, GNS, S6K, PITX2, and BMP8) were found as candidate cleft-palate genes in Chinese sturgeon. These findings contribute to our understanding of cleft palate genetics in sturgeon, while simultaneously adding to our knowledge about craniofacial development.


Assuntos
Fissura Palatina/veterinária , Doenças dos Peixes/genética , Peixes/genética , Transcriptoma , Animais , Aquicultura , China , Fissura Palatina/genética , Fissura Palatina/metabolismo , Doenças dos Peixes/metabolismo , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Peixes/metabolismo , Perfilação da Expressão Gênica , Anotação de Sequência Molecular
9.
Mitochondrial DNA B Resour ; 2(1): 54-55, 2017 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-33473715

RESUMO

The complete mitochondrial genome of Dabry's sturgeon (Acipenser dabryanus) was first determined in this study. The mitochondrial DNA is circular molecule (16,437 bp in length) with a typical vertebrate mitochondrial gene arrangement, including 13 protein-coding genes, 2 ribosomal RNA (rRNA) genes, 22 transfer RNA (tRNA) genes, and 1 non-coding control region (D-loop). All genes were encoded on the heavy strain except for ND6 and eight tRNA genes. The overall base composition of the heavy strain was A (30.2%), T (23.7%), C (29.7%), G (16.4%), and with an AT bias of 53.9%. Phylogenetic analysis based on the mitochondrial genomes of 10 related species using MEGA 5.1 indicated that A. dabryanus and A. sinensis are the most closely related species.

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