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1.
Food Chem ; 454: 139831, 2024 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-38838408

RESUMO

Diquat (DQ) and paraquat (PQ) residues in food are potential hazards to consumers' health. Point-of-care testing (POCT) of them remains challenging. Based on surface-enhanced Raman spectroscopy (SERS) technology, we developed a POCT strategy for DQ and PQ on apple surface and in apple juice. A point-of-use composite was fabricated using a piece of porous melamine sponge (MS) modified with silver nanoflowers (AgNFs), combining the specificity of the SERS fingerprint and the excellent adsorption capacity of MS. Using this dual-functional AgNFs@MS, the on-site determination of the DQ and PQ residues was completed within 3 min without pretreatment. Clear trends were observed between SERS intensity and logarithmic concentrations, with r values from 0.962 to 0.984. The limit of detection of DQ and PQ were 0.14-0.70 ppb in apple juice and on apple surface. This study provides a new point-of-use alternative for rapidly detecting DQ and PQ residues in nonlaboratory settings.


Assuntos
Diquat , Contaminação de Alimentos , Malus , Paraquat , Testes Imediatos , Prata , Análise Espectral Raman , Triazinas , Prata/química , Paraquat/análise , Triazinas/análise , Diquat/análise , Diquat/química , Malus/química , Contaminação de Alimentos/análise , Análise Espectral Raman/métodos , Resíduos de Praguicidas/análise , Resíduos de Praguicidas/química , Herbicidas/análise , Herbicidas/química , Nanopartículas Metálicas/química , Limite de Detecção , Sucos de Frutas e Vegetais/análise
2.
ACS Omega ; 9(16): 18576-18583, 2024 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-38680347

RESUMO

Paraquat (PQ) poisoning poses a significant public health concern. Unfortunately, point-of-care testing (POCT) of PQ in biofluids remains challenging. This study developed a portable kit that enables swift and reliable identification and quantification of PQ in human urine and gastric juice. The approach employed the surface-enhanced Raman scattering (SERS) technique, leveraging gold-silver core-shell nanoparticles (Au@Ag NPs) as the substrate. The kit comprised a portable Raman spectrometer and three sealed tubes containing Au@Ag NPs colloid, KI solution, and MgSO4 solution. A discernible correlation was observed between signal intensity and the logarithmic concentration, spanning from 5 to 500 µg/L in urine and 10 µg/L to 1 mg/L in gastric juice. The detection limits, calculated from the characteristic peak at 1648 cm -1, were 1.36 and 4.05 µg/L in human urine and gastric juice, respectively. Notably, this POCT kit obviated the need for pretreatment procedures, and the detection process was accomplished within 1 min, yielding satisfactory recoveries. This expeditious time frame is crucial for clinical diagnosis and rescue operations. Compared to conventional methods, this kit demonstrated real-time determinations in nonlaboratory settings. The simplicity and practicality of this POCT assay suggest its significant potential as an innovative alternative for poisoning detection applications.

3.
Ying Yong Sheng Tai Xue Bao ; 35(1): 177-185, 2024 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-38511454

RESUMO

Microbial necromass carbon (MNC) is a crucial source for stable soil carbon pool, and understanding its response to carbon inputs from both aboveground (litter) and belowground (roots) in subtropical forest soils is essential for assessing soil carbon stocks in global ecosystems. In a Castanopsis carlesii plantation at the Sanming Forest Ecosystem National Observation and Research Station in Fujian Province, we conducted an experiment with five treatments, including root removal (NR), aboveground litter removal (NL), no litter input (removals of both aboveground litter and roots, NI), double aboveground litter addition (DL), and control (CK). After seven years, we collected soil samples in the 0-10 cm soil layer to examine changes in MNC content and its contribution to soil organic carbon (SOC). Results showed that NR treatment reduced MNC, bacterial necromass carbon (BNC), and fungal necromass carbon (FNC) by 15.9%, 20.2%, and 14.5%, respectively, while other treatments did not induce significant changes. The NR, NL, NI, and DL treatments did not affect the contributions of BNC, FNC, and MNC to SOC. Correlation and path analyses revealed that litter and root carbon input treatments could alter the MNC content directly or indirectly through changing soil available substrates and microbial community structure. Our results suggested that roots exert a stronger influence on the maintenance of MNC than aboveground carbon source in the mid-subtropical plantations.


Assuntos
Ecossistema , Fagaceae , Solo/química , Carbono/análise , Microbiologia do Solo , Florestas , Bactérias
4.
ACS Appl Mater Interfaces ; 16(9): 11528-11536, 2024 Mar 06.
Artigo em Inglês | MEDLINE | ID: mdl-38386864

RESUMO

Phthalic acid esters (PAEs) are ubiquitous environmental contaminants, and their real-time monitoring and removal remain challenging. Herein, a point-of-use (POU) device integrating adsorption, surface-enhanced Raman spectroscopy (SERS), and removal strategy was developed and realized ultrafast on-site determination of PAEs and cleanup of them from water. A piece of flexible melamine sponge (MS) was coated with gold nanostars (AuNSs) and metal-organic frameworks (MOFs), thus obtaining SERS activity and adsorption capacity. Based on this multifunctional AuNSs@MOFs/MS composite, clear trends were observed between SERS signal intensity and concentration of di(2-ethylhexyl)phthalate (DEHP) and dibutyl phthalate (DBP). The method detection limits of DEHP and DBP were calculated to be 0.75 × 10-7 and 0.67 × 10-7 M in water, respectively, proving good sensitivity. Furthermore, this POU device exhibited satisfactory adsorption capacity (∼82.3 g/g for DBP and ∼90.0 g/g for DEHP), high adsorption efficiency (equilibrium in 100 s), and good regeneration capability (removal efficiency >70% after 5 cycles). The applicability of this device was verified by its good determination and removal performance in real environmental water matrices. The whole process could be completed within 5 min. This approach provides a new POU alternative for real-time monitoring and removal of PAEs in water.

5.
ACS Omega ; 8(14): 12878-12885, 2023 Apr 11.
Artigo em Inglês | MEDLINE | ID: mdl-37065026

RESUMO

The widespread use and exposure of coumatetralyl (CMTT) has led to its accumulation in the environment and organisms, causing damage to ecosystems and adverse health effects in humans. Unfortunately, achieving fast detection of CMTT remains challenging. Herein, a rapid and robust surface-enhanced Raman spectroscopy (SERS) method was developed for rapid on-site detection of CMTT in environmental water and human urine. Clear trends were observed between the signal intensity and the logarithmic concentration of CMTT, ranging from 0.025 to 5.0 µg/mL with high reproducibility. The detection limits in water and human urine were as low as 1.53 and 13.71 ng/mL, respectively. The recoveries of CMTT for environmental water and urine samples were 90.2-98.2 and 82.0-87.5%, respectively, satisfactory for practical applications. The quantitative results of this approach were highly comparable to those obtained by high-performance liquid chromatography. Most importantly, it is cost-effective, operationally simple, and without a complicated sample preparation step. Detecting CMTT in water samples took only 5 min, and the detection of urine samples was completed within 8 min. This simple yet practical SERS approach offers a reliable application prospect for on-site CMTT detection in environmental water and point-of-care monitoring of poisoned patients.

6.
Chemistry ; 29(36): e202300785, 2023 Jun 27.
Artigo em Inglês | MEDLINE | ID: mdl-37067060

RESUMO

Antibacterial photodynamic therapy (APDT) has received considerable attention owing to its superiority. ZIF-8 was used to address the poor stability of the photosensitizer Rose Bengal (RB) encapsulation to synthesize RB@ZIF-8 NPs, which were doped into a composite film with poly (ϵ-caprolactone) (PCL) and polyvinyl alcohol-quaternary ammonium chitosan (PVA-QCS) as substrates to form composite films (PQZ). The composite films exhibited excellent photodynamic sterilization and good resistance to bacterial adhesion. The tensile strength of the film increased to 43.4 MPa, which was approximately 1.8 times that of the PCL film. With the addition of SiO2 and RB@ZIF-8 NPs, the film exhibited water repellency and UV-blocking properties. RAW264.7 cells were selected using the MTT method to confirm that the composite films had excellent biocompatibility and had no significant inhibitory effect on cell growth and reproduction. PQZ multifunctional composite films show potential as novel APDT antimicrobial materials for food packaging.


Assuntos
Anti-Infecciosos , Quitosana , Dióxido de Silício , Antibacterianos/farmacologia , Antibacterianos/química , Poliésteres , Anti-Infecciosos/química , Quitosana/química , Embalagem de Alimentos
7.
Medicina (Kaunas) ; 59(2)2023 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-36837505

RESUMO

Objective: The objective of this study was to introduce a surgical technique for the percutaneous decompression of cervical stenosis (CS) using a unilateral biportal endoscopic approach and characterize its early clinical and radiographic results. Materials and Methods: Nineteen consecutive patients with CS who needed surgical intervention were recruited. All enrolled patients underwent unilateral biportal endoscopic laminectomy (UBEL). All patients were followed postoperatively for >1 year. The preoperative and final follow-up evaluations included the Japanese Orthopedic Association (JOA) score for neurological assessment, visual analogue scale (VAS) for axial pain and C2-C7 Cobb angle for cervical sagittal alignment. The postoperative complications were analyzed. Results: Thirteen males and six females were included in the analysis. The mean follow-up period was 16.3 ± 2.6 months. The mean operative time was 82.6 ± 18.4 min. Postoperative MRI and CT revealed ideal neural decompression of the treated segments in all patients. Preoperative VAS and JOA scores improved significantly after the surgery, and cervical lordosis was preserved on the postoperative images. Conclusions: UBEL was an effective surgical method for CS, which may also minimize iatrogenic damage to the posterior tension band (PTB) and help to maximize the preservation of the cervical lordosis.


Assuntos
Laminectomia , Lordose , Masculino , Feminino , Humanos , Laminectomia/efeitos adversos , Lordose/etiologia , Lordose/cirurgia , Constrição Patológica/cirurgia , Resultado do Tratamento , Endoscopia/métodos , Estudos Retrospectivos
8.
Spectrochim Acta A Mol Biomol Spectrosc ; 293: 122476, 2023 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-36787678

RESUMO

The monitoring of polybrominated diphenyl ethers (PBDEs) is of great significance owing to their high persistence, bioaccumulation, and toxicity to humans and animals. In this study, a sensitive and reproducible probe that integrates solid-phase microextraction and surface-enhanced Raman spectroscopy (SPME-SERS) was developed for screening PBDEs in multiphase specimens, including live fish, water, and electrical products. A roughed Cu fiber with an Ag layer was fabricated with dual functions. BDE-15 was readily extracted and detected on the SPME-SERS probe consisting of propanethiol-modified Ag nanoplates on a Cu wire. A clear linear relationship (R2 = 0.988) was established between the SERS intensity at 782 cm-1 and the logarithmic concentrations (from 100 ppb to 100 ppm), with a detection limit of 15 ppb. This proposed method enables continuous in vivo monitoring in fish without complicated pretreatments. The results obtained by this SPME-SERS approach were validated by high-performance liquid chromatography and showed good agreement. This "extracting and detecting" SPME-SERS method provides a potential tool to monitor the occurrence, formation, and migration of PBDEs.


Assuntos
Éteres Difenil Halogenados , Microextração em Fase Sólida , Animais , Humanos , Éteres Difenil Halogenados/análise , Microextração em Fase Sólida/métodos , Análise Espectral Raman/métodos , Água
9.
Chemistry ; 29(21): e202203458, 2023 Apr 13.
Artigo em Inglês | MEDLINE | ID: mdl-36700555

RESUMO

The management of persistent postoperative pain still remains a clinical challenge currently. Although ropivacaine (RVC) is widely used for postoperative analgesia as a local anesthetic, the short half-life makes it difficult to achieve the desired duration of analgesia. Herein, a RVC sustained-release microspheres encapsulated by zeolite imidazole framework-8 (RVC@ZIF-8) was synthesized for the first time, which prolonged the sustained-release of RVC and decreased the resulting drug toxicity. RVC can continuously release in vitro for at least 96 h with high drug loading of 30.6 % and RVC@ZIF-8 had excellent biocompatibility and low cytotoxicity. In sciatic nerve block model, the sensory block time of RVC@ZIF-8 was significantly prolonged compared with RVC, achieving more than 72 h post injection and no inflammation or lesion were found. Based on high drug loading, ideal sustained-release and superior biological safety, RVC@ZIF-8 will be a novel delivery material for local anesthetic with potential application.


Assuntos
Anestésicos Locais , Zeolitas , Ropivacaina , Amidas , Preparações de Ação Retardada , Microesferas , Imidazóis
10.
Biosens Bioelectron ; 225: 115083, 2023 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-36716572

RESUMO

Rapid detection of diquat (DQ) is essential in clinical diagnosis and rescue. Here, we developed a fast, simple-yet-practical detection strategy for the reliable identification and quantification of DQ in biological fluids. Based on surface-enhanced Raman spectroscopy (SERS), point-of-care detection was realized under the acidic condition with gold nanoparticles as the substrate. Under optimal experimental conditions, the detection limits of the strategy were 17.5 ppb and 1.99 ppm in human urine and gastric juice, respectively. High specificity and selectivity of the SERS strategy were demonstrated using common pesticides and coexisting biological substances. The method was also used to detect biofluids from 5 patients and urine samples from 10 healthy volunteers. The results were in high agreement with spectrophotometric and clinical liquid chromatography-mass spectrometry methods. The volume of urine samples required for this technique is merely 20 µL, and no preparation of the samples is required. Compared to traditional methods used in clinical settings, SERS-based methods are capable of real-time measurements that accurately provide rapid detection and response in non-laboratory settings, with great potential for on-site and point-of-care testing.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Praguicidas , Humanos , Diquat , Ouro/química , Nanopartículas Metálicas/química , Análise Espectral Raman/métodos
11.
Spectrochim Acta A Mol Biomol Spectrosc ; 285: 121880, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36130467

RESUMO

The use of Chinese herbs containing aristolochic acid can induce the exchange of adenine and thymine in gene mutations and even cause liver cancer. To eliminate the harm of aristolochic acids (AAs) to humans, a rapid and robust method of AAs screening is a prerequisite. In this work, a facile and robust Surface-enhanced Raman spectroscopy (SERS) method was used for the qualitative and quantitative detection of AAs in Chinese medicinal herbal preparations based on the mandelic acid modified Ag nanoparticles SERS substrate. Qualitative and quantitative SERS detection of Aristolochic acid I (AAI) was achieved with a good linear relationship ranging from 0.2 - 120.0 µM and a limit of detection (LOD) of 0.06 µM. The proposed method demonstrates a refined strategy for sensitivity analysis of AAs with the advantages of easy operation, time-saving, high sensitivity, and molecular specificity, making it a preferred platform for the screening of AAI in regular inspections of herbal products and regulatory supervision of the supply chain.


Assuntos
Ácidos Aristolóquicos , Medicamentos de Ervas Chinesas , Nanopartículas Metálicas , Humanos , Ácidos Aristolóquicos/análise , Preparações de Plantas/análise , Análise Espectral Raman , Medicamentos de Ervas Chinesas/análise , Prata/análise , China
12.
Comput Intell Neurosci ; 2022: 9542397, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35602621

RESUMO

Exercise-induced fatigue refers to the symptom that the body cannot continue to maintain the original training volume after a certain period of continuous training. Competitive aerobics athletes have a large amount of daily training exercise, and their bodies are prone to exercise fatigue. The health monitoring technology can monitor the physical state in real time and diagnose and treat the diseases caused by the body in time. This article aims at applying the health monitoring technology to the research of sports fatigue recovery of competitive aerobics athletes. This article first briefly introduces the theoretical review of exercise-induced fatigue, and it includes the definition, classification, and determination methods of exercise-induced fatigue, and then uses the health monitoring system to describe it, and finally conducts an experiment in the diagnosis and rehabilitation of exercise-induced fatigue. The health monitoring technology was compared with traditional medical methods, and the four aspects of speed, accuracy, recovery rate, and athlete's treatment satisfaction were tested. The experimental results show that the diagnostic accuracy based on health monitoring technology is the same as that of traditional manual medical diagnosis, reaching about 85%, which verifies its effectiveness.


Assuntos
Atletas , Esportes , Exercício Físico , Fadiga/diagnóstico , Fadiga/etiologia , Humanos , Fatores de Tempo
13.
Int J Biol Macromol ; 206: 605-613, 2022 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-35202636

RESUMO

Zeolitic imidazolate framework-8 (ZIF-8) is a type of Metal-organic frameworks (MOFs), which shows promising application in the field of bacterial infection, owing to its excellent biocompatibility. Here, we report the encapsulation of silver nanoparticles (Ag NPs) in ZIF-8, accompanied with embedding of physcion (Phy) to obtain Ag-Phy@ZIF-8 with efficient and intelligent synergistic antimicrobial capabilities. Due to the micro-acidic environment around the bacteria, the release of silver and Phy shows a controlled released. Further, the Ag-Phy@ZIF-8 is modified by hyaluronate (HA), denoted as Ag-Phy@ZIF-8@HA, which has a strong inhibitory effect on the growth of both E. coli (99.1%) and S. aureus (99.5%), with no impacting on cell growth, showing good biocompatibility. Thus, these pH-responsive biocomposites have the potential application on smart wound excipients for bacterial infections.


Assuntos
Nanopartículas Metálicas , Nanopartículas , Zeolitas , Desinfecção , Emodina/análogos & derivados , Escherichia coli , Concentração de Íons de Hidrogênio , Prata/farmacologia , Staphylococcus aureus , Zeolitas/farmacologia
14.
ACS Omega ; 7(51): 47634-47641, 2022 Dec 27.
Artigo em Inglês | MEDLINE | ID: mdl-36591153

RESUMO

There is an increasing demand for rapid detection techniques for monitoring the therapeutic concentration of voriconazole (VRC) in human biological fluids. Herein, a rapid and selective surface-enhanced Raman scatting method for point-of-care determination of VRC in human plasma was developed via a portable Raman spectrometer. This approach has enabled the quantification of the VRC spiked into human plasma at clinical relevant concentrations. A gold nanoparticle solution (Au sol) was used as the SERS substrate, and the agglomerating conditions on its sensitivity were optimized. The method involves the formation of hot spots, and the signal of VRC molecules adsorbed on the surface of the SERS hot spot was amplified by 105. The calibration curve was linear in the range of 0.02-10 ppm, with satisfactory repeatability. The limit of detection was as low as 12.3 ppb. The variation in VRC spectra over time on different substrates demonstrated good reproducibility. Notably, the salting-out extraction method developed in this study was rapid and suitable for the quantitation of drugs in biological samples. Compared with traditional methods, this approach allows for the point-of-care quantification of VRC directly in a complex matrix, which may open up new exciting opportunities for future use of the SERS technique in clinical applications.

15.
Cancer Cell Int ; 21(1): 531, 2021 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-34641863

RESUMO

BACKGROUND: The role of Diphenyldifluoroketone (EF24), a synthetic analogue of curcumin with noteworthy antitumor potential, remains unclear in non-small cell lung cancer (NSCLC). Herein, the inhibitory effect of EF24 on NSCLC and its mechanism were studied. METHODS: Cytotoxicity was measured by MTT assay, colony formation assay and xenograft model. Cell apoptosis and reactive oxygen species (ROS) level were quantified by flow cytometer. Protein level was detected by western blot assay. Mitochondria and autophagosomes were observed using transmission electron microscope and confocal microscopy. RESULTS: In-vitro, EF24 significantly induced proliferation inhibition, apoptosis, mitochondrial fission and autophagy of NSCLC cell lines. These cytotoxic effects were significantly attenuated by two reactive oxygen species (ROS) scavengers, indicating its anti-cancer effects largely depend on ROS accumulation. In-vivo, EF24 inhibited tumor growth in a dose-dependent manner. Moreover, no pathological changes of heart, lung, spleen, kidney and liver of mice were observed. Collectively, EF24 induced ROS accumulation, in turn activates cell apoptosis, and then exerts its cytotoxicity on NSCLC cells. CONCLUSIONS: The results showed that EF24 exerted cytotoxicity against NSCLC via ROS accumulation. Thus, EF24 might serve as a potential anti-cancer agent for the treatment of NSCLC.

16.
ACS Appl Mater Interfaces ; 13(25): 29916-29925, 2021 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-34139846

RESUMO

The poor stability and low catalytic activity of NH2-UiO-66 in basic solutions require the reactions to be conducted in acidic solutions, which seriously hinders its potential photocatalytic application. Herein, we report that NH2-UiO-66 coated with two-dimensional covalent organic frameworks (COFs) via imine bond connection presents not only high photocatalytic activity but also high stability and adaptability to the solution environment. The NH2-UiO-66/COF hybrid material was fabricated through the Schiff base reaction of NH2-UiO-66 with 4,4',4″-(1,3,5-triazine-2,4,6-triyl)trianiline (TAPT) and 2,4,6-triformylphloroglucinol (TP). The hybrid material showed high stability in an alkaline environment, with only 4.7% of NH2-UiO-66 decomposed after the photocatalytic reaction. The optimum photocatalytic H2 evolution rate was 8.44 mmol·h-1·g-1 when triethanolamine was used as an electron-donating agent. The results presented here illustrate the possibility for effectively improving both the photocatalytic performance and stability of NH2-UiO-66 by coupling with COFs.

17.
Chemistry ; 27(51): 13059-13066, 2021 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-34190368

RESUMO

Covalent triazine frameworks (CTFs) with two-dimensional structures have exhibited promising visible-light-induced H2 evolution performance. However, it is still a challenge to improve their activity. Herein, we report π-conjugation-linked CTF-1/GO for boosting photocatalytic H2 evolution. The CTF-1/GO hybrid material was obtained by a facile low-temperature condensation of 1,4-dicyanobenzene in the presence of GO. The results of photocatalytic H2 evolution indicate that the optimum hybrid, CTF-1/GO-3.0, exhibited an H2 evolution rate of 2262.4 µmol ⋅ g-1 ⋅ h-1 under visible light irradiation, which was 9 times that of pure CTF-1. The enhanced photocatalytic performance could be attributed to the fact that GO in CTF-1/GO hybrids not only acts as an electron collector and transporter like a "bridge" to facilitate the separation and transfer of photogenerated charges but also shortens the electron migration path due to its thin sheet layer uniformly distribution over CTF-1. This work could help future development of novel conjugated CTF-based composite materials as high-efficiency photocatalyst for photocatalysis.

18.
Mol Immunol ; 134: 210-217, 2021 06.
Artigo em Inglês | MEDLINE | ID: mdl-33819783

RESUMO

Exosomes are protein-containing vesicles that are secreted into the blood to mediate important biological and pathological processes. The present study enrolled 86 patients with advanced hepatocellular carcinoma (HCC) and 60 healthy controls. Serum exosome levels of the patient group were significantly elevated compared with the healthy control group (P = 0.001). No significant differences were observed between patients with serum alpha-fetoglobulin levels of less than 200 ng/mL and more than 200 ng/mL. In vitro, dendritic cells (DCs) were activated by exosomes and could promote T cell proliferation, exhibiting a killing effect on HepG2 cells. In addition, DCs loaded with tumor exosomes (DC-TEX) showed an antitumor effect in a subcutaneous tumor model. This study shows exosome levels in patients with HCC to be significantly higher than in healthy individuals. Furthermore, exosomes derived from serum of patients with advanced HCC function as tumor antigens.


Assuntos
Antígenos de Neoplasias/imunologia , Carcinoma Hepatocelular/imunologia , Exossomos/imunologia , Neoplasias Hepáticas/imunologia , Idoso , Animais , Proliferação de Células , Células Dendríticas/imunologia , Feminino , Xenoenxertos , Humanos , Ativação Linfocitária/imunologia , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Pessoa de Meia-Idade , Linfócitos T/imunologia , alfa-Fetoproteínas/metabolismo
19.
J Plant Physiol ; 253: 153260, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32846310

RESUMO

The endophytic Bacillus amyloliquefaciens YTB1407 was previously reported to promote the growth of sweet potato (Ipomoea batatas cv. Yanshu 25). Here, we demonstrate in both in vitro and pot trial assays that pre-treatment with YTB1407 suspension could enhance resistance against root rot disease and black rot disease, caused by Fusarium solani Mart. Sacc. f. sp. batatas McClure and Ceratocystis fimbriata Ell. & Halst on sweet potato, respectively. When seedlings were infected with fungal pathogens at 10 days post irrigation, pre-treatment with YTB1407 suspension decreased these pathogens and YTB1407 bacterial biomass in sweet potato roots. The pre-treatment activated the expression of salicylic acid (SA)-responsive PR-1 gene, raised SA content, and reduced hydrogen peroxide (H2O2) in the host to resist F. solani, while it enhanced the expression levels of SA-responsive NPR1 and PR1 genes and increased SA content to resist C. fimbriata. The disease resistance control effect initiated by pre-treatment with YTB1407 for root rot pathogen (F. solani) was better than for black rot pathogen (C. fimbriata). The results indicated that Bacillus amyloliquefaciens YTB1407 played a pivotal role in enhancing resistance to two fungi pathogens in sweet potato, through production of some antifungal metabolites to decrease infection in the early stage as well as induction of SA-dependent systemic resistance.


Assuntos
Bacillus amyloliquefaciens/fisiologia , Resistência à Doença , Fusarium/fisiologia , Peróxido de Hidrogênio/metabolismo , Ipomoea batatas/microbiologia , Doenças das Plantas/microbiologia , Antifúngicos/metabolismo , Endófitos , Ipomoea batatas/imunologia , Doenças das Plantas/imunologia , Raízes de Plantas/imunologia , Raízes de Plantas/microbiologia , Ácido Salicílico/metabolismo , Plântula/imunologia , Plântula/microbiologia
20.
RSC Adv ; 10(13): 7360-7367, 2020 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-35492192

RESUMO

Sodium alginate (ALG) composites with ZIF-8 and niflumic acid (NIF) were prepared by a one-pot method at room temperature and characterized by FTIR, SEM and XRD studies. In the composite, ZIF-8 was used as a highly connected node in a supercrosslinked polymer network. In addition, the material exhibits good antibacterial activity against Staphylococcus aureus and Escherichia coli in vitro. Compared to the original ALG membrane and ZIF-8, the ZIF-NIF-ALG membrane has the following advantages: stronger antibacterial properties; slow release of Zn(ii); high drug loading; and longer sustained release time. This research introduces new concepts for the design and manufacture of various antimicrobial membranes and broadens the range of applications of MOFs.

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