Biodegradable Active Packaging Material Containing Grape Seed Ethanol Extract and Corn Starch/κ-Carrageenan Composite Film.

An active film composed of corn starch/κ-carrageenan and ethanolic grape seed extract (0, 1, 3, and 5 wt% of GSE on corn starch basis) were successfully prepared using the solvent casting technique. The effects of the different concentrations of ethanolic grape seed extract (GSE) on the physicochemical properties, antioxidant properties, and antibacterial properties of CS/κC films were analyzed. The results showed that the addition of GSE inhibited the recrystallization of starch in the composite film. The glass transition temperature of composite film is 121.65 °C. With the addition of GSE, the surface roughness of the composite film increased, and the cross-section displayed a stratification phenomenon. Meanwhile, when GSE was added to the composite film, the tensile strength of the composite film decreased (3.50 ± 0.27 MPa), the elongation at break increased (36.87 ± 2.08%), and the WVP increased (1.58 ± 0.03 g mm/m2·d· kPa). With the increase of the concentration of GSE in the composite film, the a* value and b* value of the composite film increase, the L* value decreases, and the opacity increases. The lipid oxidation test proved that the composite films containing 1% GSE has a significant inhibitory effect on the oxidation of lard (p < 0.05). The above results indicate that the GSE can be used as a food-grade packaging material and has a good application prospect in the food industry.

Preparation and Performance Characterization of a Composite Film Based on Corn Starch, κ-Carrageenan, and Ethanol Extract of Onion Skin.

Using corn starch (CS) and κ-carrageenan(κC) as the raw material and active composite, respectively, films containing different concentrations of ethanol extract of onion skin were prepared. The effects of different concentrations of ethanol extract of onion skin (EEOS) on the physicochemical properties, as well as the antioxidant and antibacterial properties, of CS/κC films were also discussed. The addition of ethanol extract of onion skin inhibited the recrystallization of starch molecules in the composite films. It affected the microstructure of the composite films. The color of the composite films was deepened, the brightness was reduced, and the opacity was increased. Water vapor permeability increased, tensile strength decreased, and elongation at the break increased. The glass-transition temperature decreased. The clearance of DPPH radicals and ABTS cation radicals increased. Moreover, when the concentration of EEOS was 3%, the antioxidant effect of the films on oil was greatly improved and could effectively inhibit Staphylococcus aureus and Escherichia coli. The above results showed that adding ethanol extract of onion skin improved the physicochemical properties and biological activities of the CS/κC composite films, so CS/κC/EEOS composite films can be used as an active packaging material to extend food shelf-life. These results can provide a theoretical basis for the production and application of corn starch/κ-carrageenan/ethanol extract of onion skin composite films.

Effect of ethanol extract of black soybean coat on physicochemical properties and biological activities of chitosan packaging film.

Chitosan (CS) with an ethanol extract of black soybean coat (EBSC) was prepared, and its physicochemical properties and antioxidant and antibacterial activities were tested. The results showed that EBSC significantly increased the thickness and UV-Vis light barrier ability of the CS-based films, while the swelling degree, water vapor permeability, and tensile strength decreased. The CS-EBSC films had smooth surfaces, compact cross-sections, and no cracks, and they had higher crystallinity than the CS film. Fourier transform-infrared spectroscopy indicated that there were noncovalent bonds (hydrogen bonds) between EBSC and CS. Furthermore, the CS-EBSC III film presented a stronger ABTS radical scavenging ability (66.58%) and could effectively inhibit Bacillus subtilis, Escherichia coli, and Staphylococcus aureus. The lipid oxidation test proved that CS-EBSC films significantly reduced the peroxide value of lard. The results above indicate that CS-EBSC films could be used as an active packaging material to improve the shelf life of food.

A photoelectrochemical biosensor based on SnO2 nanoparticles for phosphatidylcholine detection in soybean oil.

A photoelectrochemical (PEC) biosensor based on SnO2 nanoparticles (SnO2 NPs) was developed and applied for phosphatidylcholine (PC) detection in soybean oil. SnO2 NPs were grown on an indium tin oxide (ITO) electrode, polythionine (PTh) was electropolymerized on the surface of ITO/SnO2 NPs, and choline oxidase (ChOx) was immobilized to prepare the ITO/SnO2 NPs/PTh/ChOx electrode. The developed PEC biosensor can detect PC under visible light irradiation. The experimental conditions for PC detection were as follows: 1.8 mg mL-1 ChOx concentration, 0.5 V bias voltage, 18 mW cm-2 light intensity, and pH 6. The PEC biosensor had a detection limit of 0.005 mM (S/N = 3) and a detection range from 0.03 mM to 4 mM. This PEC biosensor based on SnO2 NPs was applied to detect PC in soybean oil. The recovery rate tested by the standard addition method was 95.2-107.4%. These findings were consistent with the results obtained by high-performance liquid chromatography (HPLC). Therefore, the proposed PEC biosensor based on SnO2 NPs has excellent reproducibility, stability, and great potential applications in the PEC analysis of PC in soybean oil.

Hepatoprotective effects of apple polyphenol extract on aluminum-induced liver oxidative stress in the rat.

This study was undertaken to determine the effectiveness of apple (Ralls) polyphenol extract (APE) in modulating aluminum chloride (AlCl3) induced hepatotoxicity in rats. The rats were distributed among 4 groups and fed different diets with or without AlCl3 (171.8 mg Al·kg(-1)·day(-1)) and APE (200 mg·kg(-1)·day(-1)) for 10 weeks. The activities of superoxide dismutase and catalase as well as the levels of glutathione and ATP synthesis were decreased by comparison with the control, while the activities of transaminases in serum, the levels of Al, and ATP hydrolysis were increased significantly in the liver of the Al-treated group. Furthermore, abnormal changes in the histological structure of the liver were observed in the Al-treated group. However, these toxic effects of Al were significantly reduced when the rats were fed diets supplemented with APE. This suggests that APE plays a role in the reduction of the toxic effects from Al in rats.