Complete Genome Insights into Lactococcus petauri CF11 Isolated from a Healthy Human Gut Using Second- and Third-Generation Sequencing.

Lactococcus petauri CF11 was originally isolated from the gut of healthy humans. To determine the underlying molecular and genetic mechanisms of the probiotic potential of CF11, we performed complete genome sequencing, annotation, and comparative genome analysis. The complete genome of L. petauri CF11 comprised of 1,997,720 bp, with a DNA G+C content of 38.21 mol% containing 1982 protein coding genes and 16 rRNA operons. We found that 1206 genes (56.05%) were assigned a putative function using the gene ontology (GO) resource. The gene products of CF11 were primarily concentrated in molecular function and biological processes, such as catalysis, binding, metabolism, and cellular processes. Furthermore, 1,365 (68.87%) genes were assigned an illative function using COGs. CF11 proteins were associated with carbohydrate transport and metabolism, and amino acid transport and metabolism. This indicates that CF11 bacteria can perform active energy exchange. We classified 1,111 (56.05%) genes into six KEGG functional categories; fructose-bisphosphate aldolase and the phosphoenol pyruvate:phosphotransferase system (PTS), which are necessary in producing short-chain fatty acids (SCFAs), were excited in the carbohydrate metabolic pathway. This suggests that L. petauri CF11 produces SCFAs via glycolysis. The genomic island revealed that some regions contain fragments of antibiotic resistance and bacteriostatic genes. In addition, ANI analysis showed that L. petauri CF11 had the closest relationship with L. petauri 159469T, with an average nucleotide consistency of 98.03%. Taken together, the present study offers further insights into the functional and potential role of L. petauri CF11 in health care.

[Prevalence and risk factors for anxiety among inhabitants in the relief centers after the 5 *12 Sichuan earthquake].

OBJECTIVE: To investigate the prevalence and risk factors for anxiety among inhabitants in the relief centers one month after the 5 * 12 Sichuan earthquake and to formulate intervention strategies. METHODS: A total of 402 tent inhabitants aged > or = 18 years were randomly selected from 13 relief centers of 4 townships in Jiangyou city. Data were collected by Self-rating Anxiety Scale (SAS) and risk factor questionnaires. RESULTS: The prevalence of anxiety among inhabitants in the relief centers was 22.1% (95% CI: 18.2%-26.6% ) (89/402). In single factor analysis, female, aged > or = 40 years, family per-capita monthly income of < 600 Yuan, education level of less than junior high school, inconvenient living conditions, history of chronic diseases, limited living space in tents, shortage of goods, and sex life being interrupted etc might increase anxiety. In multi-factor analysis, female (OR = 2.921), inconvenient living conditions (OR = 2.475), history of chronic diseases (OR = 3.997), and limited living space in tents (OR = 2.982) were the risk factors for anxiety. CONCLUSION: Inhabitants in the relief centers exhibited higher prevalence of anxiety as compared with the general population. Measures to improve the living conditions of the relief centers, including guarantee of water, electricity and material supply, sewage treatment, living space and privacy, and early psychological interventions were recommended for prevention and relief of anxiety.

[Investigation of anxiety among 402 tent inhabitants in Jiangyou city after the 5.12 Sichuan earthquake].

OBJECTIVE: To investigate the prevalence of anxiety among tent inhabitants one month after the 5.12 Sichuan earthquake as to formulating intervention strategies. METHODS: A stratified random sampling method was adopted. Inclusion criteria were: inhabitants, aged above 18 years old, living in tent in 13 relief centers of 4 townships in Jiangyou city; and only one person from each tent/family could be enrolled in this study. A total of 402 subjects were interviewed with Self-rating Anxiety Scale (SAS). SAS scores were compared between different groups and symptomatic differences between anxiety and non-anxiety victims were analyzed by stepwise multiple regression analysis. RESULTS: The prevalence of anxiety among tent inhabitants was 22.1% (89/402). A higher rate of anxiety was observed in senior age group than in younger age group (F = 7.03, P < 0.01). Age with > or = 50 years (42.85 +/- 11.15), 40-49 years (41.57 +/- 10.30), 30-39 years (37.99 +/- 9.66), 18-29 years (36.62 +/- 9.92). Female (43.13 +/- 10.45) had a high rate of anxiety than male (36.80 +/- 9.88) (t = -6.09, P < 0.01). The most common symptoms were anxiety (79.4%, 319/402), fatigue (68.2%, 274/402), phobia (65.7%, 264/402), sleep disorders (65.4%, 263/402), unfortunate feelings (65.2% , 262/402), akathisia (59.7%, 240/402), and fear (58.2%, 234/402). Stepwise multiple regression analysis revealed that insane feelings(t = 5.37, P < 0.01), trembling(t = 5.33, P < 0.01), dyspnea (t = 4.28, P < 0.01), unfortunate feelings (t = 3.87, P < 0.01), headache(t = 3.85, P < 0.01), facial flushing (t = 3.48, P <0.01), fatigue (t = 3.27, P < 0.01), dizziness (t = 2.79, P < 0.01), frequent micturition (t = 2.41, P < 0.05), and akathisia (t = 2.31, P < 0.05) were more frequently experienced in the anxiety victims than non-anxiety victims. CONCLUSION: Tent inhabitants in the post-earthquake relief centers exhibit a higher prevalence of anxiety as compared with the general population. Much attention should be paid to elderly, female, and those who have developed specific symptoms such as insane feelings, trembling, dyspnea, etc. Meanwhile, some intervention measures should be timely taken.