Bacillus subtilis TO-A extends the lifespan of Caenorhabditis elegans.

Clostridium butyricum TO-A, Enterococcus faecium T-110, and Bacillus subtilis TO-A are sold as oral probiotic preparations and reportedly exhibit many beneficial effects on the health of hosts, including humans and livestock. In this study, we compared the ability of these clinically applied probiotic bacteria with Escherichia coli OP50 in extending the lifespan of Caenorhabditis elegans. To compare the C. elegans lifespan-extending effects of the three bacteria, experiments were performed using a nematode growth medium containing a small amount of trypticase soy agar. The maximum lifespans of worms fed C. butyricum TO-A, E. faecium T-110, or B. subtilis TO-A increased by 11, 12, and 26%, respectively, compared with worms fed E. coli OP50. In addition, we conducted a metabolomic analysis of methanol extracts of B. subtilis TO-A cells, which exhibited the strongest lifespan-extending effect on C. elegans among the probiotic bacteria tested in this study. As a result, 59 candidate substances involved in extending the lifespan of C. elegans were identified in B. subtilis TO-A cells.

[Adsorption Characteristics of Phosphorus Wastewater on the Synthetic Ferrihydrite].

The synthetic ferrihydrite, FerrorMox (FM), was used as adsorbent for removing phosphorus from wastewater. SEM, EDS, XRD, FTIR and Raman were used to characterize FM, and the results indicated that FM was amorphous 2 L ferrihydrite and was composed of Fe, O, Ca and Si, etc. Afterwards, FM was applied to adsorb phosphorus from wastewater, and the adsorption performance, influence factors and adsorption mechanism were investigated. The phosphorus removal rate reached 99.14% under the condition of adsorption time of 60 min, initial pH phosphate solution of 2, relative dosing quantity of 7 g·L-1, reaction temperature of 25℃, initial concentration of 10 mg·L-1, and solution volume of 50 mL. Adsorption isotherms were well fitted with the Langmuir isothermal adsorption model at different temperature with the correlation coefficient reaching above 0.95. The thermodynamic parameters showed that the phosphorus adsorption by FM was a spontaneous endothermic reaction. The phosphate removal kinetics well followed both pseudo-first-order model and pseudo-second-order model. About 99% of phosphate adsorbed on FM could be desorbed in 0.1 mol·L-1 NaOH solution. Therefore, FM was a promising absorbent material for the removal of phosphate from waste water.