Combined effects of elevated CO2 and warmer temperature on limitations to photosynthesis and carbon sequestration in yellow birch.

Elevated CO2 and warmer temperature occur simultaneously under the current climate change. However, their combined effects on the photosynthetic traits in boreal trees are not well understood. This study investigated the morphological and photosynthetic responses of yellow birch (Betula alleghaniensis Britt.) to a combined treatment of CO2 and temperature (ambient, ACT (400 µmol mol-1 CO2 and current temperature) vs elevated, ECT (750 µmol mol-1 CO2 and current +4 °C temperature)). It was found that ECT significantly reduced leaf-area based photosynthetic rate (An), maximum Rubisco carboxylation rate (Vcmax), photosynthetic electron transport rate (Jmax), leaf nitrogen concentration, respiration and mesophyll conductance. There were two interesting findings: first, the primary mechanism of photosynthetic limitation shifted from Ribulose-1,5-bisphosphate (RuBP) carboxylation (related to Vcmax) to RuBP regeneration (related to Jmax) in response to ECT, leading to decreased transition point (Ci-t and An-t) from RuBP carboxylation to regeneration; second, the increase in total leaf area in response to ECT more than compensated for the downregulation of leaf-area based photosynthesis, leading to greater biomass in ECT than in ACT. We proposed a new protocol for evaluating photosynthetic limitations by comparing the relative relationship between the transition point (Ci-t and An-t) and the photosynthetic rate at growth CO2 (Ci-g and An-g). Furthermore, we found that Jmax (RuBP regeneration) was the primary limitation to An under ECT.

Live imaging of RNA and RNA splicing in mammalian cells via the dcas13a-SunTag-BiFC system.

Dynamic tracking of the localization of RNA molecules (nucleus and/or cytoplasm) and RNA splicing in living cells plays an important role in understanding their functions. However, a lack of dynamic imaging and high background fluorescence have been reported in the fluorescence in situ hybridization (FISH). Here, we developed a new tool, the dcas13a-SunTag-BiFC system, which fused the dLwacas13a and SunTag systems. dLwacas13a is used as a tracker to target specific RNAs, while SunTag recruits split Venus fluorescent proteins to label targeted RNAs. Our results showed that 4 × NLS-dCas13a-24 × SunTag-BiFC and 2 × NLS- dCas13a-24 × SunTag-BiFC systems can be used for imaging of endogenous RNA foci in the nucleus (Xist) and cytoplasm (Ppib and stress granules) in living cells, respectively. Compared to 12x MS2-MCP system, the dcas13a-SunTag-BiFC system showed a better performance of mRNA foci tracking in live cells. Furthermore, we confirmed the premature termination codon (PTC)-induced exon skipping of Oxt RNA using the dcas13a-SunTag-BiFC and MS2-MCP systems in the nucleus. Thus, the dcas13a-SunTag-BiFC system will facilitate the study of RNA localization in living cells and provide new insights into RNA translocation and splicing.

Short-term effects of thinning on the understory natural environment of mixed broadleaf-conifer forest in Changbai Mountain area, Northeast China.

BACKGROUND: The understory natural environment is critical in affecting the succession and recovery process of vegetation, stand structure, and species composition of forest. The thinning intensity could significantly change the forest microclimates and soil properties, therefore, to analyze the effects of thinning intensity on the understory natural environment of forest is of important significance for promoting the ecological benefits of thinning. METHODS: A total of 16 fixed sample plots with different thinning intensities were established in the mixed broadleaf-conifer forest in Jiaohe, situated in Changbai Mountain area, Northeast China, and the forest microclimates and soil properties were investigated after 4 years since the establishment of the sample plots. RESULTS: The results showed that the high intensity thinning significantly decreased the leaf area index from 4.13 (unthinned plot) to 2.21 (high intensity thinned plot), and the air temperature was increased by thinning from May to July. Comparing with the unthinned plot, thinning caused a rise of temperature (ranging from 2.11 to 6.74 °C, depending on the intensity of thinning) in May. However, it showed cooling effect in September and October. Besides, the air moisture of thinning plots was lower than the control plot in May and October, when the density of leaves is lower in the forest, and it even decreased 20.27% after thinning. The thinning intensity had no significantly effect on water content and organic carbon in forest soils, and only the bulk density in the top-layer soils in high intensity thinning plot was remarkably increased. Total nitrogen in soil was increased by different intensities of thinning, and the availability of nutrients for nitrogen, phosphorus and potassium in some soils were also affected.