Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 5 de 5
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Sci Rep ; 11(1): 18521, 2021 09 16.
Artigo em Inglês | MEDLINE | ID: mdl-34531469

RESUMO

Plant kinases containing the LysM domain play important roles in pathogen recognition and self-defense reactions. And it could recognize microbe-associated molecules including chitin and other polypeptides. The white tip nematode Aphelenchoides besseyi is a migratory parasitic nematode that infects plant shoots. It is distributed over almost all rice-producing areas and causes up to 50% economic losses. The rice OsRLK3 gene was a defense-related LysM kinase gene of rice. This study showed that the rice LysM kinase OsRLK3 could be induced by flg22, jasmonic acid, salicylic acid, and chitin. An interaction gene, Ab-atps from A. besseyi, was identified by screening the interaction between the rice gene OsRLK3 and an A. besseyi cDNA library using yeast two-hybrid screening. Ab-atps is a novel ATP synthase gene with a full length of 1341 bp, coding for 183 amino acids. The mRNA of Ab-atps was located in the esophagus and reproductive system of A. besseyi. The expression of Ab-atps was assessed at different developmental stages of the nematode and found to be the highest in the juvenile, followed by the egg, female, and male. Reproduction was significantly decreased in nematodes treated with Ab-atps double-stranded RNA (dsRNA) (p < 0.05). Transient expression experiments showed that Ab-ATPS-GFP was distributed in the nucleus, cytoplasm, and cell membrane, and Ab-ATPS-GFP triggered plant cell death. OsRLK3 was expressed significantly higher at 0.5 day and 1 day (p < 0.05) in rice plants inoculated with nematodes treated with Ab-atps dsRNA and gfp dsRNA for 0.5-7 days, respectively. Further, OsRLK3 expression under Ab-atps dsRNA treatment was significantly lower than with gfp dsRNA treatment at 0.5 day (p < 0.05) and significantly higher than with gfp dsRNA treatment at 1 day (p < 0.05). These results suggest that rice OsRLK3 could interact with A. besseyi Ab-atps, which plays an important role in growth, reproduction, and infection of the nematode. Our findings provide a theoretical basis to further understand the parasitic strategy of A. besseyi and its interaction mechanism with host plants, suggesting new ideas and targets for controlling A. besseyi.


Assuntos
Adenosina Trifosfatases/genética , Interações Hospedeiro-Parasita/genética , Oryza/parasitologia , Doenças das Plantas/parasitologia , Rabditídios/genética , Animais , Oryza/genética , Doenças das Plantas/genética
2.
Funct Integr Genomics ; 20(3): 367-381, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-31713833

RESUMO

White-tip nematode, Aphelenchoides besseyi is a kind of widely distributed migratory parasitic nematode that can infect plant shoots. Transcriptome sequencing of plant parasitic nematodes and their host plants is helpful for understanding their interaction relationship. This study first reported expression patterns of defense-related genes in rice, and rice transcriptomes at different periods after infection with A. besseyi. The result showed that the defense response pathways of rice changed obviously in the early stage of A. besseyi infection, including upregulated salicylic acid and jasmonate pathways and a downregulated ethylene pathway. Transcriptome analysis results suggested that A. besseyi infection was associated with the downregulation of multiple genes related to photosynthesis with possible suppression of the photosynthetic activity. It suggested that the photosynthesis system of rice could be suppressed by infections of migratory nematodes, including A. besseyi and Hirschmanniella oryzae, but was stimulated by that of a sedentary nematode, Meloidogyne graminicola, by comparing our study with the reported transcriptome. OS09G0417800 (OsWRKY62) might play an important role in the interaction of migratory nematodes and rice. It also indicated that the infection strategy of both A. besseyi and the reported migratory nematode H. oryzae was similar to that of the fungal pathogen Magnaporthe grisea. These results provided an interesting starting point to elucidate the mechanism of the interaction between rice and A. besseyi, as well as the host and migratory plant nematodes.


Assuntos
Regulação da Expressão Gênica de Plantas , Oryza/genética , Fotossíntese , Doenças das Plantas/genética , Transcriptoma , Tylenchoidea/patogenicidade , Animais , Regulação para Baixo , Oryza/metabolismo , Oryza/parasitologia , Doenças das Plantas/parasitologia
3.
Sci Rep ; 9(1): 6277, 2019 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-31000750

RESUMO

Radopholus similis is an important migratory endoparasitic nematode, severely harms banana, citrus and many other commercial crops. Little is known about the molecular mechanism of infection and pathogenesis of R. similis. In this study, 64761 unigenes were generated from eggs, juveniles, females and males of R. similis. 11443 unigenes showed significant expression difference among these four life stages. Genes involved in host parasitism, anti-host defense and other biological processes were predicted. There were 86 and 102 putative genes coding for cell wall degrading enzymes and antioxidase respectively. The amount and type of putative parasitic-related genes reported in sedentary endoparasitic plant nematodes are variable from those of migratory parasitic nematodes on plant aerial portion. There were no sequences annotated to effectors in R. similis, involved in feeding site formation of sedentary endoparasites nematodes. This transcriptome data provides a new insight into the parasitic and pathogenic molecular mechanisms of the migratory endoparasitic nematodes. It also provides a broad idea for further research on R. similis.


Assuntos
Proteínas de Helminto/genética , Plantas/parasitologia , Transcriptoma/genética , Tylenchoidea/genética , Animais , Regulação da Expressão Gênica , Filogenia , Doenças das Plantas/genética , Doenças das Plantas/parasitologia , Plantas/genética , Tylenchoidea/patogenicidade
4.
PLoS One ; 13(6): e0199935, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29958285

RESUMO

The rice white tip nematode, Aphelenchoides besseyi, is widely distributed in rice planting areas worldwide and causes serious economic losses. Cathepsin genes have been demonstrated to have importance in studying the reproduction, development, pathogenicity, and control methods of plant nematodes. In this paper, a novel cathepsin B gene, Ab-cb-1, was found and cloned. The Ab-cb-1 gene was 1347 bp in length and encodes 369 amino acids. The Ab-CB-1 protein contains characteristic occluding loops but no signal peptide. A homology analysis showed that Ab-CB-1 had the highest identity value (64%) to the known amino acid sequence of cathepsin B-like cysteine protease 6 from Toxocara canis. When Ab-cb-1 was expressed in a prokaryotic system, the protein massed approximately 45 kDa and could decompose carrot callus. Ab-cb-1 mRNA was localized in the nematode intestine. The relative expression level of Ab-cb-1 in the A. besseyi Ab-S24 population, which had high reproductivity, was approximately 6.9 times that in the Ab-N10 population, which had low reproductivity, and the difference was significant (p<0.05). The Ab-cb-1 expression level was highest in females; the expression levels in males, juveniles and eggs were 30%, 12.2% and 5% of that in females, respectively, and the differences were significant among all four stages (p<0.05). Nematodes of the Ab-S24 population were treated with Ab-cb-1 dsRNA for 12 h, 24 h, 36 h and 48 h, and their reproduction decreased with increasing time. These results demonstrated that Ab-CB-1 was a digestive enzyme with hydrolytic protease properties and that Ab-cb-1 played an important role in the reproduction of A. besseyi.


Assuntos
Catepsina B/genética , Proteínas de Helminto/genética , Rabditídios/genética , Caracteres Sexuais , Animais , Catepsina B/metabolismo , Feminino , Proteínas de Helminto/metabolismo , Masculino , Rabditídios/enzimologia
5.
Huan Jing Ke Xue ; 36(12): 4348-57, 2015 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-27011967

RESUMO

To investigate the discrepancy of distributions of major gas pollutants and aerosol size between weekdays and weekends, trace gases (SO2, NO2, CO and O3), PM (PM10 and PM2.5) and aerosol spectral distributions at 10 nm-10 µm were monitored in May 2015 at Nanhu district in Jiaxing. The results showed that the gas pollutants of SO2, NO2, CO and O3 had significant weekend effects in Jiaxing. The O3 concentration on daytime of weekends was lower than that of weekdays. The peak of O3 occurred at 14:00 on weekends, which was 1 hour later than that on weekdays. The weekend effect of PM2.5 was observed to be much more obvious compared to that of PMPM2.5. The ratios of PMPM2.5/PM10 were 0.7 and 0.6 on weekends and weekdays, respectively. The aerosol number concentration was mostly concentrated at particle size below 500 nm, with values of 16,602 cm⁻³ and 23,309 cm⁻³ on weekends and weekdays. The most remarkable weekend effect was found for aerosol number concentration in nuclei mode, for surface area concentration in nuclei and coarse mode, and for mass concentration in accumulation and coarse mode. The spectra of aerosol number concentration, surface area concentration and mass concentration showed unimodal, trimodal and four-peak distributions, respectively.


Assuntos
Poluentes Atmosféricos/análise , Gases/análise , Material Particulado/análise , Aerossóis , China , Tamanho da Partícula , Estações do Ano , Fatores de Tempo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...