Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 3 de 3
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Angew Chem Int Ed Engl ; 63(1): e202315861, 2024 Jan 02.
Artigo em Inglês | MEDLINE | ID: mdl-37985247

RESUMO

Atherosclerosis is a lipoprotein-driven disease, and there is no effective therapy to reverse atherosclerosis or existing plaques. Therefore, it is urgently necessary to create a noninvasive and reliable approach for early atherosclerosis detection to prevent initial plaque formation. Atherosclerosis is intimately associated with inflammation, which is accompanied by an excess of reactive oxygen species (ROS), leading to cells requiring more glutathione (GSH) to resist severe oxidative stress. Therefore, the GSH-hydrolyzed protein γ-glutamyl transpeptidase (GGT) and the ROS-hypobromous acid (HBrO) are potential biomarkers for predicting atherogenesis. Hence, to avoid false-positive diagnoses caused by a single biomarker, we constructed an ingenious sequence-activated double-locked TP fluorescent probe, C-HBrO-GGT, in which two sequential triggers of GGT and HBrO are meticulously designed to ensure that the probe fluoresces in response to HBrO only after GGT hydrolyzes the probe. By utilization of C-HBrO-GGT, the voltage-gated chloride channel (CLC-1)-HBrO-catalase (CAT)-GGT signaling pathway was confirmed in cellular level. Notably, the forthcoming atherosclerotic plaques were successfully predicted before the plaques could be observed via the naked eye or classical immunofluorescent staining. Collectively, this research proposed a powerful tool to indicate the precise position of mature plaques and provide early warning of atherosclerotic plaques.


Assuntos
Aterosclerose , Placa Aterosclerótica , Humanos , Placa Aterosclerótica/diagnóstico por imagem , gama-Glutamiltransferase/metabolismo , Corantes Fluorescentes/metabolismo , Fluorescência , Espécies Reativas de Oxigênio , Aterosclerose/diagnóstico
2.
Chem Commun (Camb) ; 59(74): 11017-11027, 2023 Sep 14.
Artigo em Inglês | MEDLINE | ID: mdl-37667841

RESUMO

Various bioactive substances are simultaneously involved in physiological processes, and research on the synergistic effect of them can promote the study of pathological mechanisms. To achieve this purpose, several small-molecule double-locked logic gate fluorescence probes have been developed recently. They overcome many shortcomings of the traditional "single-signal" fluorescent probes, with fluorescence that can be activated by two analytes of interest order-independently or order-dependently with one output. In this review, we summarize recently published small-molecule double-locked logic gate probes for the optical detection of two bioactive substances in living systems. We envision that this review will attract significant attention from researchers to exploit more powerful functional double-locked logic gate probes.


Assuntos
Corantes Fluorescentes , Fluorescência
3.
Chem Commun (Camb) ; 57(47): 5838-5841, 2021 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-34008637

RESUMO

We synthesized an ultrasensitive probe TP-Golgi for the two-photon ratiometric fluorescence imaging of Golgi polarity. Probe TP-Golgi possesses a large Stokes shift, excellent sensitivity and good selectivity to quantitatively detect environmental polarity. By application of TP-Golgi, we found that the Golgi polarity increased obviously in the kidneys of mice with AKI.


Assuntos
Injúria Renal Aguda/diagnóstico por imagem , Polaridade Celular/efeitos dos fármacos , Corantes Fluorescentes/química , Complexo de Golgi/efeitos dos fármacos , Imagem Óptica , Fótons , Injúria Renal Aguda/induzido quimicamente , Linhagem Celular , Corantes Fluorescentes/síntese química , Complexo de Golgi/patologia , Humanos , Estrutura Molecular
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...