Co-composting with cow dung and subsequent vermicomposting improve compost quality of spent mushroom.

In order to determine a feasible degrading process for spent mushroom (SMS) with high lignin content, the present work used cow dung (CD), SMS, and a mixture of CD and SMS as substrates and evaluated the effects of vermicomposting on the microflora and the quality of composting products. Bacterial (R2 = 0.548, P = 0.001) and fungal (R2 = 0.314, P = 0.005) community both were different between composting and vermicomposting. Vermicomposting and substrates affected enzyme activities indirectly by affecting ammonium, pH, total carbon, richness, and bacterial community composition. These results suggested that appropriate regulation of environmental factors may increase microbial activity. An increase in ion-exchange capacity (up to 139.8%), pH (6.9%), and nitrate (71.1%) and a decrease in total carbon (31.2%) and carbon/nitrogen ratio (32.1%) in vermicomposting indicated that earthworms could further improve product quality. Co-composting with CD and integrated subsequent vermicomposting efficiently promoted the maturity of SMS.

Conversion of rainforest into agroforestry and monoculture plantation in China: Consequences for soil phosphorus forms and microbial community.

Microbial communities and their associated enzyme activities affect quantity and quality of phosphorus (P) in soils. Land use change is likely to alter microbial community structure and feedback on ecosystem structure and function. This study presents a novel assessment of mechanistic links between microbial responses to land use and shifts in the amount and quality of soil phosphorus (P). We investigated effects of the conversion of rainforests into rubber agroforests (AF), young rubber (YR), and mature rubber (MR) plantations on soil P fractions (i.e., labile P, moderately labile P, occluded P, Ca P, and residual P) in Hainan Island, Southern China. Microbial community composition and microbial enzyme were assayed to assess microbial community response to forest conversion. In addition, we also identified soil P fractions that were closely related to soil microbial and chemical properties in these forests. Conversion of forest to pure rubber plantations and agroforestry system caused a negative response in soil microorganisms and activity. The bacteria phospholipid fatty acid (PLFAs) levels in young rubber, mature rubber and rubber agroforests decreased after forest conversion, while the fungal PLFAs levels did not change. Arbuscular mycorrhizal fungi (AMF) (16:1w5c) had the highest value of 0.246µmol(gOC)-1 in natural forest, followed by rubber agroforests, mature rubber and young rubber. Level of soil acid phosphatase activity declined soon (5 years) after forest conversion compared to natural forest, but it improved in mature rubber and agroforestry system. Labile P, moderately labile P, occluded P and residual P were highest in young rubber stands, while moderately labile, occluded and residual P were lowest in rubber agroforestry system. Soil P fractions such as labile P, moderately labile P, and Ca P were the most important contributors to the variation in soil microbial community composition. We also found that soil P factions differ significantly among the four transformation systems. Soil labile P faction and its potential sources (moderately labile P, occluded P, and residual P) were positively correlated with NO3-, but negatively correlated with AMF, suggesting that these properties play key roles in P transformation. Our study indicated that land use had an impact on microbial community composition and functions, which consequently influenced soil phosphorus availability and cycling.

Effect of exogenous abscisic acid on the level of antioxidants in Atractylodes macrocephala Koidz under lead stress.

This study hypothesized that the positive or negative effects of exogenous abscisic acid (ABA) on oxidative stress caused by lead were dose dependent. The effects of different levels of ABA (2.5, 5, and 10 mg L(-1)) on lead toxicity in the leaves of Atractylodes macrocephala were studied by investigating plant growth, soluble sugars, proteins, lipid peroxidation, and antioxidative enzymes. Excess Pb inhibited root dry weight, root length, and the number of lateral roots, but increased shoot growth. In addition, lead stress significantly decreased the levels of chlorophyll pigments, protein, and activities of superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and peroxidase (POD). Different levels of ABA significantly increased SOD, CAT, POD, and APX activities, but decreased the level of hydrogen peroxide and malondialdehyde in nonstressed plants. Exogenous application of 2.5 mg L(-1) ABA detoxified the stress-generated damages caused by Pb and also enhanced plant growth, soluble sugars, proteins, and all four antioxidant enzyme activities but reduced Pb uptake of lead-stressed plant compared to lead treatment alone. However, the toxic effects of Pb were further increased by the applications of 5 and 10 mg L(-1) ABA. The levels of antioxidants caused by a low concentration of exogenous ABA might be responsible for minimizing the Pb-induced toxicity in A. macrocephala.

[Effects of Zanthoxylum bungeanum leaf extract on soil microbe quantity and enzyme activities].

In a pot experiment, the aqueous extract of 10 years old Zanthoxylum bungeanum's leaves was used to water Z. bungeanum seedlings to test its effects on soil microbes and enzyme activities. The results showed that the leaf extract decreased the total amount of microbes and the quantity of bacteria, fungi and actinomyces in rhizosphere soil, but increased the total amount of microbes in non-rhizosphere soil. After watering with the extract, the proteinase, invertase and acid phosphatase activities in rhizosphere soil were considerably lower than those in nonrhizosphere soil, while catalase and polyphenol oxidase activities were in adverse. Soil proteinase activity had a significant positive correlation with invertase activity, but a significant negative correlation with actinomyces amount. Soil polyphenol oxidase activity had a significant negative correlation with invertase activity, but a positive correlation with the total amount of microbes and the quantity of bacteria, fungi and actinomyces. Soil actinomyces only had significant correlations with proteinase, polyphenol oxidase and invertase activities and fungi.

[Effect of combined application of bioorganic manure and inorganic nitrogen fertilizer on soil nitrogen supplying characteristics].

Pot experiment was carried out to study the effect of application bioorganic manure on soil nitrogen supplying characteristics. Compared with the application of raw rice straw, a combined application of bio-organic manure and inorganic nitrogen fertilizer could boost the content of microbial biomass carbon and nitrogen, and the increased percentage could be 300-400%. A strong immobilization of nitrogen by microorganisms was always followed by a net N mineralization, which was mostly favorable for the growth and development of plant, and improved the use efficiency of nitrogen fertilizer.