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1.
Kaohsiung J Med Sci ; 36(3): 178-185, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31709719

RESUMO

Gastric cancer (GC) is the second leading cause of cancer-related death worldwide. Studies have shown that miR-922 facilitates the development of various diseases and tumors. However, the role of miR-922 in GC and related molecular mechanisms are still unrevealed. Current study indicated that miR-922 was overexpressed in GC tissues and cells. The survival rate of patients in high miR-922 expression group is significantly lower than that in low miR-922 expression group. In addition, overexpression of miR-922 observably restrained the apoptosis of SGC7901 cells and promoted SGC7901 cell proliferation, migration, and invasion. TargetScan predicted that suppressors of cytokine signaling 1 (SOCS1) was a potential target of miR-922. miR-922 upregulation profoundly inhibited the expression of SOCS1. Furthermore, the mRNA level of SOCS1 in GC tissues was significantly lower than that in adjacent tissues, indicating that miR-922 promoted the proliferation, invasion, and migration, and inhibited apoptosis of SGC7901 cells by downregulating the level of SOCS1. In conclusion, miR-922 may have potential for diagnosis of GC.


Assuntos
MicroRNAs/metabolismo , Neoplasias Gástricas/metabolismo , Proteína 1 Supressora da Sinalização de Citocina/metabolismo , Cicatrização/fisiologia , Apoptose/genética , Apoptose/fisiologia , Western Blotting , Linhagem Celular Tumoral , Movimento Celular/genética , Movimento Celular/fisiologia , Proliferação de Células/genética , Proliferação de Células/fisiologia , Citometria de Fluxo , Regulação Neoplásica da Expressão Gênica/genética , Humanos , MicroRNAs/genética , Invasividade Neoplásica/genética , Reação em Cadeia da Polimerase em Tempo Real , Neoplasias Gástricas/genética , Proteína 1 Supressora da Sinalização de Citocina/genética , Cicatrização/genética
2.
Zhonghua Kou Qiang Yi Xue Za Zhi ; 43(5): 278-80, 2008 May.
Artigo em Chinês | MEDLINE | ID: mdl-18953911

RESUMO

OBJECTIVE: To study the influence of lactational dioxin exposure (2, 3, 7, 8-tetrachlorodibenzo-p-dixon, TCDD) on development of alveolar bone in SD rat offspring. METHODS: The rats of TCDD exposure group and control group were sacrificed and the alveolar bone with molars of PD60 rats in the two groups were embedded in resin. The sections were observed by fluorescent microscope. The alveolar bone formation was evaluated by histological examination, tetracycline fluorescence marker and quantitative histomorphometry. The indices of quantitative histomorphometry were compared. RESULTS: The trabecular structure of alveolar bone was looser in TCDD exposure group than in the control group. The tetracycline fluorescence markers were more disorganized in TCDD group. The indices of quantitative histomorphometry of alveolar bone between two groups showed significantly different. CONCLUSIONS: Lactational 2,3,7,8-TCDD exposure decreased the quality and quantity of alveolar bone in SD rat offspring. It is suggested that dioxins exposure may interrupt the spatial configuration.


Assuntos
Exposição Materna , Dibenzodioxinas Policloradas/toxicidade , Alvéolo Dental/efeitos dos fármacos , Alvéolo Dental/crescimento & desenvolvimento , Animais , Feminino , Lactação , Gravidez , Ratos , Ratos Sprague-Dawley , Alvéolo Dental/patologia
3.
Artigo em Chinês | MEDLINE | ID: mdl-15748508

RESUMO

OBJECTIVE: To investigate the effects of lead exposure at different levels in utero on the teeth eruption and enamel development of rat offsprings. METHODS: 27 pregnant SD rats were divided into three groups randomly: high level lead group (HLG), low level lead group (LLG) and control group with nine rats in each group. The three groups from the gestation day to the end of the gestation were given either deionized water in control group or deionized water containing 200 mg/L Pb2+ as lead acetate through drinking method in high level lead experimental group and 50 mg/L Pb2+ as lead acetate through drinking method in low level lead experimental group. The incisors of newborn rats were marked at the level of the gingival papilla on the 26th day after birth. On the 36th day, the incisors of newborn rats were marked again at the same level. Then the rat offsprings were killed and their blood was collected for lead analysis. The mandible incisors of rat offspring were separated and the content of Pb in incisors was determined by using a graphite furnace atomic absorption spectrometric method. The teeth of rat offspring were observed and the distance between two marks were measured by means of stereomicroscope. The ratio of calcium to phosphate of enamel of rat offspring was compared by electron probe microanalyses. RESULTS: The level of blood lead in 200 mg/L, 50 mg/L treated rat offspring groups was higher than that in control group. The tooth lead of 200 mg/L, 50 mg/L treated rat offspring groups [(77.3 +/- 6.3), (27.8 +/- 4.5) microg/g] were higher than the control [(6.6 +/- 0.8) microg/g, P < 0.01]. Compared with the control group, the teeth of lead exposure experimental groups were smaller and severity of attrition was obvious and pulpal perforations were often observed. These appearances was more distinct in rats of high level lead experimental group. The incisors of lead-treated rat offspring erupted [(0.25 +/- 0.08), (0.30 +/- 0.09) mm/d] more slowly than control ones [(0.39 +/- 0.09) mm/d, P < 0.01]. The ratio of calcium to phosphate (Ca/P) decreased with the increase of lead exposure. It was found that Ca/P in lead exposure experimental groups (1.68 +/- 0.54), (1.37 +/- 0.47) was significantly lower than that in the control group (2.14 +/- 0.33). CONCLUSION: Lead exposure in utero affects the normal eruption of teeth and enamel formation and the degree was related with the lead exposure level.


Assuntos
Órgão do Esmalte/efeitos dos fármacos , Chumbo/efeitos adversos , Exposição Materna/efeitos adversos , Efeitos Tardios da Exposição Pré-Natal/patologia , Erupção Dentária/efeitos dos fármacos , Animais , Feminino , Gravidez , Ratos , Ratos Sprague-Dawley
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