De Novo Design of a Highly Stable Ratiometric Probe for Long-Term Continuous Imaging of Endogenous HClO Burst.

Long-term continuous imaging of endogenous HClO burst is of great importance for the elucidation of various physiological or pathological processes. However, most of the currently reported HClO probes have failed to achieve this goal due to their insufficient photobleaching resistance under a laser source. Herein, a highly stable ratiometric probe, HFTC-HClO 1, which is capable of continuously monitoring endogenous HClO burst over a long period of time, has been judiciously developed. Briefly, the de novo development of HFTC-HClO 1 mainly involved three main steps: (1) novel coumarins (HFTC 1-5) were designed and synthesized; (2) the most stable scaffold, HFTC 3, was selected through dye screening and cell imaging validation; and (3) based on HFTC 3, three candidate HClO probes were constructed, and HFTC-HClO 1 was finally selected due to its superior sensing properties toward HClO. Furthermore, HFTC-HClO 1 can quantitatively measure HClO levels in various real samples with excellent recovery (>90.4%), and the use of HFTC-HClO 1-coated test strips for qualitative analysis of HClO in real samples was also achieved. In addition, the application of HFTC-HClO 1 for long-term continuous monitoring of intracellular HClO burst was successfully demonstrated. Significantly, HFTC-HClO 1 was able to visualize HClO generated in the rheumatoid arthritis mouse model.

Investigation of 2π isolated skyrmion pinning using exchange bias.

The 2π isolated chiral skyrmion is a magnetic configuration. Since the total topological charge is zero, the 2π isolated skyrmion driven by a spin-polarized current propagates strictly along the racetrack. The manipulation of 2π-skyrmion, e.g., pinning/depinning at a specific position of the racetrack, is significant. Here, we investigated the 2π-skyrmion pinning in a racetrack using exchange bias. A series of transversal AFM wires were set above the ferromagnetic (FM) racetrack. Spin waves were employed to induce 2π-skyrmion motion to study the dynamics of the 2π-skyrmion pinning. The AFM wires induce exchange bias at the AFM/FM crossing points, which can act as pinning sites. The working window for a 2π-skyrmion in a racetrack was investigated as a function of the exchange bias field, the frequency and amplitude of the oscillating magnetic field for exciting spin waves. The interaction mechanism between the 2π-skyrmion and the exchange bias was also studied. This work may provide guidance for the design of next-generation spintronics.