[Relation of intermediate-conductance Ca(2+)-activated K(+) channels with ability of proliferation, migration, invasion and IgE secretion of multiple myeloma cells].

This study was aimed to investigate the effects of the intermediate-conductance Ca(2+)-activated K(+) (IKCa1) channels on the proliferation, migration, invasion ability and monoclonal immunoglobulin (IgE) secretion of multiple myeloma (MM) cells. Trypan blue exclusion was used to evaluate the impact of clotrimazole (CLO, an inhibitor of the KCa1) on the survival ability of MM cell line U266; transwell chamber and matrigel experiments were used to evaluate the impact of CLO on the ability of the migration and invasion of U266 cells; the influence of CLO on IgE secretion in U266 cells was detected by ELISA. The results showed that small dose of CLO ( ≤ 1.0 µmol/L) could not inhibit the viability of U266 cells. The Transwell and Matrigel invading tests displayed that the cell number moving into lower chamber of transwell decreased after U266 cells treated with small dose of CLO ( ≤ 1.0 µmol/L). After treating the cells with 1.00 µmol/L CLO for 24 h and 48 h, the concentration of IgE in cell supernatant was (4.98 ± 0.39) and (4.38 ± 0.32) ng/ml, while those in control group were (15.41 ± 1.88) and (19.73 ± 2.01) ng/ml, respectively, suggesting significant difference between them(P < 0.05). It is concluded that CLO can decrease the ability of migration and monoclonal immunoglobulin secretion of multiple myeloma cells by blocking the IKCa1, thus this study provides a new think for the targeted therapy of MM.

Recovery of isoflavone aglycones from soy whey wastewater using foam fractionation and acidic hydrolysis.

The purpose of this work was to recover isoflavone aglycones from industrial soy whey wastewater, where the isoflavone aglycones mainly existed in the form of ß-glycosides. First, foam fractionation was used for effectively concentrating the total soy isoflavones, including isoflavone aglycones and ß-glycosides, from the wastewater. Fourier transform infrared spectroscopy indicated the existence of complexes of soy isoflavones and soy proteins. When soy proteins were used as the collectors, a high enrichment ratio of 3.68 was obtained under the optimal operating conditions of temperature of 50 °C, pH of 5.0, volumetric air flow rate of 100 mL/min, and loading liquid height of 400 mm. Subsequently, acidic hydrolysis was used for hydrolyzing ß-glycosides in the foamate into aglycones. Using response surface methodology, a hydrolytic percentage could reach 96% under the optimum hydrolysis conditions of hydrolytic temperature of 80 °C, hydrochloric acid concentration of 1.37 mol/L, and hydrolytic time of 90 min.

[Relationships of nucleotide, amino acid sequence and secondary structure of protein and molecular design].

May the structure information of protein be obtained from the corresponding nucleotide sequence? For this question, a computer program was used to conduct a statistical analysis about the clustering phenomena of amino acids. In this method, 20 kinds of amino acid were classified into 2 types according to the number of hydrogen bonds formed by middle base of their correlation codons. It can be seen that amino acid has a rather great possibility of neighboring on another of its class and this assembly has a tendency to forming specific secondary structure. A sequence was designed and its secondary structure was predicted by this prediction software.