Colorimetric detection of electrolyte ions in blood based on biphasic microdroplet extraction.

BACKGROUND: Timely diagnosis and prevention of diseases require rapid and sensitive detection of biomarkers from blood samples without external interference. Abnormal electrolyte ion levels in the blood are closely linked to various physiological disorders, including hypertension. Therefore, accurate, interference-free, and precise measurement of electrolyte ion concentrations in the blood is particularly important. RESULTS: In this work, a colorimetric sensor based on a biphasic microdroplet extraction is proposed for the detection of electrolyte ions in the blood. This sensor employs mini-pillar arrays to facilitate contact between adjacent blood microdroplets and organic microdroplets serving as sensing phases, with any color changes being monitored through a smartphone's colorimetric software. The sensor is highly resistant to interference and does not require pre-treatment of the blood samples. Remarkably, the sensor exhibits exceptional reliability and stability, allowing for rapid enrichment and detection of K+, Na+, and Cl- in the blood within 10 s (Cl-), 15 s (K+) and 40 s (Na+) respectively. SIGNIFICANCE: The colorimetric sensor based on biphasic microdroplet extraction offers portability due to its compact size and ease of operation without the need for large instruments. Additionally, it is location-independent, making it a promising tool for real-time biomarker detection in body fluids such as blood.

Vertical observation and analysis on rapid formation and evolutionary mechanisms of a prolonged haze episode over central-eastern China.

To clarify the rapid formation and evolutionary mechanisms of an extremely severe and persistent haze and fog (HF) episode that occurred in central-eastern China from Dec 20 to 25, 2015, a novel campaign was conducted and vertical profiles of wind, temperature, light extinction coefficient (LEC) and PM2.5 concentration were used to analyze the rapid formation and evolutionary mechanisms of this HF episode. The substantial downward transportation of regional pollution from high layers and stagnant weather conditions favorable for the local pollution accumulation were the two main causes of the rapid increase in pollutant concentration. Southwest wind speeds of 4m/s between 300 and 600m and obvious downward flows were observed, whereas the southwest wind speeds were low below 300m, and strong temperature inversion with intensity of 4.5°C/100m expanded vertically to a height of 600m. Two peaks of PM2.5 concentration were observed at 200 and 700m, corresponding to 235 and 215µg/m3, respectively. The frequent change in wind direction and wind speeds resulted in the fluctuation of PM2.5 concentration. The turbulence within lower layers of the troposphere was enhanced by easterly and northerly winds which decreased the pollution level; however, the strength and stretching height of the winds were insufficient to fully clear the air of pollutants. The PM2.5 concentration revealed 2-high concentration layers in the vertical direction. The maximum concentration layer was below 100m, while the second high-concentration layer was at 400m.

ShRNA-mediated silencing of the Ndc80 gene suppress cell proliferation and affected hepatitis B virus-related hepatocellular carcinoma.

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common and lethal malignancies in the world, and hepatitis B virus (HBV) has been well established to cause HCC. Ndc80 complex is a conserved mitotic regulator dedicated to ensuring faithful chromosome segregation and plays an important role in inducing tumor formation. However, its role in HCC caused by HBV infection remains unclear. METHODS: Immunohistochemistry (IHC), Western blot (WB), and real-time qRT-PCR were used to measure the expression of Ndc80 in HBV-related HCC tissues. Ndc80-specific short hairpin RNA (shRNA) was used to knock-down Ndc80 expression in the hepatoma cell line HeG2 and HepG2.2.15, which is stable transcribed with HBV genome. Furthermore, the effect of Ndc80 on cellular proliferation and growth were examined, respectively. RESULTS: The expression level of Ndc80 was remarkably up-regulated in HBV-related HCC tissues. Down-regulation of Ndc80 expression suppressed HBV replication. With cell counting and the MTS assay, cellular proliferation and growth of Ndc80 knocking-down cell line was shown to be effectively restrained. CONCLUSION: This study suggests that Ndc80 may play an important role in the process of HBV-related HCC, and that it may be a potential biological treatment target in the future.