Lipid metabolism-related long noncoding RNA RP11-817I4.1 promotes fatty acid synthesis and tumor progression in hepatocellular carcinoma.

BACKGROUND: Hepatocellular carcinoma (HCC) is one of the most common types of tumors. The influence of lipid metabolism disruption on the development of HCC has been demonstrated in published studies. AIM: To establish an HCC prognostic model for lipid metabolism-related long non-coding RNAs (LMR-lncRNAs) and conduct in-depth research on the specific role of novel LMR-lncRNAs in HCC. METHODS: Correlation and differential expression analyses of The Cancer Genome Atlas data were used to identify differentially expressed LMR-lncRNAs. Quantitative real-time polymerase chain reaction analysis was used to evaluate the expression of LMR-lncRNAs. Nile red staining was employed to observe intracellular lipid levels. The interaction between RP11-817I4.1, miR-3120-3p, and ATP citrate lyase (ACLY) was validated through the performance of dual-luciferase reporter gene and RIP assays. RESULTS: Three LMR-lncRNAs (negative regulator of antiviral response, RNA transmembrane and coiled-coil domain family 1 antisense RNA 1, and RP11-817I4.1) were identified as predictive markers for HCC patients and were utilized in the construction of risk models. Additionally, proliferation, migration, and invasion were reduced by RP11-817I4.1 knockdown. An increase in lipid levels in HCC cells was significantly induced by RP11-817I4.1 through the miR-3120-3p/ACLY axis. CONCLUSION: LMR-lncRNAs have the capacity to predict the clinical characteristics and prognoses of HCC patients, and the discovery of a novel LMR-lncRNAs, RP11-817I4.1, revealed its role in promoting lipid accumulation, thereby accelerating the onset and progression of HCC.

Bone Mesenchymal Stem Cell-Derived Exosome-Enclosed miR-181a Induces CD4+CD25+FOXP3+ Regulatory T Cells via SIRT1/Acetylation-Mediated FOXP3 Stabilization.

Bone marrow mesenchymal stem cells (BMSCs) have been identified as a potential therapeutic approach to immune-related diseases. Here, we show that BMSC-derived exosomes promote FOXP3 expression and induce the conversion of CD4+ T cells into CD4+CD25+FOXP3+ Treg cells, which is significant for immunosuppressive activity. We found that miR-181a-5p is upregulated in BMSC-derived exosomes and can be transferred to CD4+ T cells. In CD4+ cells, miR-181a directly targets SIRT1 and suppresses its expression. Moreover, downregulated SIRT1 enhances FOXP3 via protein acetylation. In conclusion, our data demonstrated that BMSC-derived exosomal miR-181a is critical in the maintenance of immune tolerance. Furthermore, our results reveal that BMSC-derived exosomal miR-181a induces the production of CD4+CD25+FOXP3+ Treg cells via SIRT1/acetylation/FOXP3.

Efficacy and Safety of Glucocorticoids Versus Placebo as an Adjuvant Treatment to Surgery in Chronic Subdural Hematoma: A Systematic Review and Meta-Analysis of Randomized Controlled Clinical Trials.

BACKGROUND: Chronic subdural hematoma (CSDH) is a common neurosurgical disease with a high recurrence rate, especially among the elderly. Glucocorticoids have been tested for the treatment of CSDH in observational studies and randomized clinical trials. METHODS: We systematically searched the PubMed, Embase, and Cochrane Central Register of Controlled Trials database for randomized trials from the earliest date available to May 23, 2021 that had compared glucocorticoids and placebo as a postoperative treatment of CSDH. Trials were included if the study participants were aged ≥18 years and had had CSDH after surgery. The relative risk (RR) was used to evaluate the clinical outcomes. RESULTS: We included 5 eligible randomized controlled trials with a total of 1251 patients. The findings showed that the use of adjuvant glucocorticoid therapy can effectively reduce the recurrence risk of CSDH compared with placebo (RR, 0.40; 95% confidence interval [CI], 0.28-0.58; P < 0.001). No significant differences were found between the glucocorticoid and placebo groups regarding favorable neurological outcomes (RR, 1; 95% CI, 0.93-1.08; P = 0.92). We found that the use of adjuvant glucocorticoids resulted in a significant increase in psychiatric symptoms (RR, 3.22; 95% CI, 1.83-5.64; P < 0.001). No significant differences were found for infection between the 2 groups (RR, 1.86; 95% CI, 0.56-6.14; P = 0.31). CONCLUSIONS: Glucocorticoid therapy can effectively reduce the recurrence risk of CSDH after surgery without an increase in the postoperative infection rate. However, significantly increased psychiatric symptoms were reported in the glucocorticoid group.

TGF-ß1-overexpressing mesenchymal stem cells reciprocally regulate Th17/Treg cells by regulating the expression of IFN-γ.

Transforming growth factor (TGF)-ß1 and mesenchymal stromal cells (MSCs) are two effective immunosuppressive agents for organ transplantation technology. This study aims to explore the molecular mechanism of TGF-ß1-overexpressed MSCs on T cell immunosuppression. To achieve that, BM-MSCs were isolated from canine bone marrow, and their osteogenic differentiation and surface markers were detected. The TGF-ß1 gene was transferred into lentivirus and modified MSCs (TGF-ß1/MSCs) by lentivirus transfection. Furthermore, TGF-ß1/MSCs were co-cultured with T cells to investigate their effect on differentiation and immune regulation. Results showed that TGF-ß1/MSCs significantly downregulated the proportion of CD4+ CD8+ T cells in lymphocytes and significantly upregulated the proportion of CD4+ CD25+ T cells. Moreover, TGF-ß1/MSCs significantly upregulated the expression of IL-10 in CD4+ T cells and downregulated the expression of IL-17A, IL-21, and IL-22. Meanwhile, interferon-γ (IFN-γ) neutralizing antibody blocked the effects of TGF-ß1/MSCs on the differentiation inhibition of Th17. Overall, our results confirm the strong immunosuppressive effect of TGF-ß1/MSCs in vitro and demonstrate that IFN-γ mediates the immunosuppressive effect of TGF-ß1/MSC.

Occurrence of microplastic in the water of different types of aquaculture ponds in an important lakeside freshwater aquaculture area of China.

Aquaculture ponds are exposed to numerous potential microplastic sources, but studies on their microplastic pollution are still limited. Various culture species may influence the occurrence of microplastic in ponds. In the present study, the occurrence of microplastics was studied in aquaculture ponds for fish, crayfish, and crab, as well as in the natural lake near the aquaculture area around the Honghu Lake, which is the principal freshwater aquaculture area of China. The microplastic abundances ranged from 87 items/m3 to 750 items/m3 in the aquaculture ponds, and 117 items/m3 to 533 items/m3 in the lake. The crab ponds contained higher abundances of microplastics than fish ponds and the nearby natural lakes. Microplastics that were between 100 and 500 µm and larger than 1000 µm in size were predominant in the ponds and nearby lakes, whereas the proportion of microplastics that were smaller than 100 µm was higher in crab ponds than those in other ponds. Fragments and fibers were the predominant shapes of microplastics in the ponds. The proportion of smaller microplastics in the ponds had a positive correlation with the proportion of fragment microplastics. The results of this study implied that differences in the use of plastics in various types of aquaculture ponds might affect their microplastic pollution characteristics. Microplastics discharged from ponds to nearby lakes through drainage processes require attention in further studies.

Interactions between dicyandiamide and periphytic biofilms in paddy soils and subsequent effects on nitrogen cycling.

Dicyandiamide (DCD) is commonly used as nitrification inhibitors which has the potential to reduce nitrogen loss from paddy soils. In paddy systems, periphytic biofilms are commonly presented at the soil/water interface and show significant effects on nutrient cycling. However, the interaction between DCD and periphytic biofilms in paddy and subsequent effects on nitrogen cycling is unclear. In this work, microcosm experiments were carried out to study the interaction between the periphytic biofilms and DCD and the potential influence on nitrogen cycling from in paddy. Results showed that DCD affected the development of periphytic biofilms, while the presence of periphytic biofilms accelerated DCD degradation. Results also showed DCD application reduced nitrification potential mainly by inhibiting ammonia-oxidizing bacteria (AOB). Higher DCD dosage increased NH3 volatilization loss. However, presence of periphytic biofilm reduced the NH3 volatilization loss but increased denitrification. Our work contributes to a better understanding on the nitrogen cycling processes in paddy, and provides useful information for the improvement of nitrogen utilization efficiency and the control of non-point source pollution.