Extensive necrotizing fasciitis of scrotum and abdominal wall: Report of two cases and a review of the literature.

The incidence rate of necrotizing fasciitis(NF) is low, but it has a high mortality rate. At present, it lacks experience in clinical treatment in municipal and county-level hospitals, insufficient awareness of disease risk, lack of experience in disease surgical intervention, and lack of a set of mature treatment norms and standards. Most patients have no time to transfer to a higher hospital for treatment. In January and April 2022, two cases of large-scale necrotizing fasciitis of the scrotum and abdominal wall were treated in the Department of Urology of Weifang people's Hospital respectively and were clinically cured after active surgical debridement combined with broad-spectrum antibiotics. Through the retrospective analysis of the diagnosis and treatment of two cases of necrotizing fasciitis, this paper analyzes and summarizes the scope of surgical debridement of NF, postoperative dressing changing skills, timing of multiple debridements, application and timing of vacuum sealing drainage(VSD), and the combined use of antibiotics. To provide experience for clinical diagnosis and treatment of necrotizing fasciitis.

Clinical Study on the Efficacy of Microwave Ablation (MA) in the Treatment of Stage I Renal Clear Cell Carcinoma by CT and MRI Imaging.

We have proposed an effective mechanism to corroborate the efficacy of microwave ablation (MA) in the treatment of stage I renal clear cell carcinoma in this paper. For this purpose, a total of 96 patients with stage I renal clear cell carcinoma presented in our hospital from May 2018 to January 2021 were randomly divided into CT group (n = 48) and MRI group (n = 48). Patients in both groups were treated with microwave ablation after pathological diagnosis. Patients in the CT group received enhanced CT examination to monitor the therapeutic effect; in contrast, patients in the MRI group received MRI examination to monitor their therapeutic effect. The focus areas before and after tumor microwave ablation were compared between the two groups. The patients were followed up to 1 year after the operation, and the microwave ablation inactivation rates of the two groups were compared according to the postoperative follow-up results. There was no significant difference between CT and MRI in the levels of long and short diameter before and after microwave ablation of renal clear cell carcinoma (P > 0.05). In the CT group, CT examination was performed within 24 hours after microwave ablation treatment, and 44 of 48 ablation lesions showed complete ablation. The remaining 4 lesions showed nodular heterogeneous enhancement in the arterial phase, indicating that the tumor remained. Microwave ablation was performed on the residual lesions during the operation, and then enhanced CT was performed again to show that the lesions were ablated completely. In the MRI group, MRI examination was performed within 24 hours after microwave ablation treatment, and 45 of 48 ablation lesions showed complete ablation. The remaining 3 lesions showed nodular heterogeneous enhancement in the arterial phase, indicating that the tumor remained. Microwave ablation was performed on the residual lesions during the operation, and MRI examination showed that the lesions were ablated completely. The patients were followed up to 1 year after the operation, and the microwave ablation inactivation rate of the two groups was compared according to the postoperative follow-up results as the gold standard. The inactivation rate of microwave ablation in the CT group was 89.58 (43/48). The inactivation rate of microwave ablation in the MRI group was 100.00% (48/48). The inactivation rate of microwave ablation in the MRI group was higher than that in the CT group (χ 2 = 5.275, P = 0.021).

Choline-based deep eutectic solvent combined with EDTA-2Na as novel soil washing agent for lead removal in contaminated soil.

Lead-contaminated soil was cleaned through ethylene-diamine-teraacetic acid disodium salt (EDTA-2Na) combined with diluted deep eutectic solvent (DES) which was prepared by mixing choline chloride with ethylene glycol. The influences of leaching temperature, leaching time, liquid-solid (L/S) ratio, concentration of EDTA-2Na, water-DES ratio, and the molar ratio of choline chloride-ethylene glycol (Ch-E) on the leaching rate of lead were investigated. The mineral phases of the soil and DES before and after washing were analyzed using scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). The changes to the DESs before and after dissolving lead nitrate (Pb(NO3)2) were analyzed by high resolution mass spectrometry (HRMS) and nuclear magnetic resonance (NMR). Hydrogen bonds and EDTA-2Na in the Ch-M system resulted in the conversion of Pb(NO3)2 to other complex ions such as [Pb·Ch-E]- and [Pb·EDTA-2Na]- and other complex ions due to the dissolution of the washing agent. The results showed that the soil mineral phase did not change significantly and up to 95.79% of Pb could be washed under temperature, time, L/S ratio, EDTA-2Na concentration, DES/water ratio, Ch-E molar ratio, and stirring speed conditions of 40 °C, 2 h, 6, 0.02 M, 2, 0.75 and 300 rpm, respectively. The hydrogen bonds and EDTA-2Na may play a key role in the remediation of lead-contaminated soil by a washing agent. This research describes a rapid, efficient, and environmentally friendly method for remediation of lead-contaminated soil.

[Characteristics of DNA adsorption and desorption in variable and constant charge soil colloids].

The characteristics of adsorption and desorption of DNA by Red soil colloid, Latosol colloid, Chao colloid and Cinnamon colloid at different pH values were studied using a batch method. It showed that there was an increase of solution pH after adsorption of DNA by the four soil colloids in both NaCl and KCl electrolyte systems. The increasing ranges of pH values were in order of Red soil colloid > Latosol colloid > Chao colloid > Cinnamon colloid, and NaCl electrolyte system > KCl electrolyte system. The amounts of DNA adsorption on soil colloids decreased with the increase of pH value. The maximum amounts of DNA adsorption in different colloids were about 13.1-14.8 microg x mg(-1) when pH values were 2-4. The decreasing ranges of the amounts of DNA adsorption were about 5.5 microg x mg(-1) in NaCl electrolyte system and 2.1 Mg x mg(-1) in KCl electrolyte system in Red soil colloid and Latosol colloid after the rising of equilibrium solution pH from 4.2 to 8.6, whereas the remarked decreasing ranges of the adsorption amounts of DNA were about 8.3-12.2 microg x mg(-1) on Chao colloid and Cinnamon colloid in two electrolyte systems. The decreasing ranges of DNA adsorption were in order of the constant charge (Chao soil and Cinnamon) colloids > the variable charge (Red soil and Latosol) colloids. The differences of desorption on the variable and the constant charge colloids are very significant while the DNA adsorbed was desorbed with NaOAc solution and NaH2 PO4 solution. The desorption percent desorption of DNA as NaH2PO4 desorbent was 23.5%-40.2% larger on the variable charge colloids than 8.8%-21.6% on the constant charge of colloids at the three different solution pH values of 3, 5 and 7, while that as NaOAc desorbent was 72.3%-85.9% larger on the constant charge colloids than 10%-24.5% on the variable charge colloids. These results implied that the ligand exchange played a more important role in DNA adsorption on the variable charge colloids, and electrostatic interactions did on the constant charge colloids. This is the differences of DNA adsorption and desorption on different charge colloid surfaces.