Assessing the Performance of a Classification-Based Vulnerability Analysis Model.

In this article, a classification model based on the majority rule sorting (MR-Sort) method is employed to evaluate the vulnerability of safety-critical systems with respect to malevolent intentional acts. The model is built on the basis of a (limited-size) set of data representing (a priori known) vulnerability classification examples. The empirical construction of the classification model introduces a source of uncertainty into the vulnerability analysis process: a quantitative assessment of the performance of the classification model (in terms of accuracy and confidence in the assignments) is thus in order. Three different app oaches are here considered to this aim: (i) a model-retrieval-based approach, (ii) the bootstrap method, and (iii) the leave-one-out cross-validation technique. The analyses are presented with reference to an exemplificative case study involving the vulnerability assessment of nuclear power plants.

DNA enzyme ED5 depletes egr-1 and inhibits neointimal hyperplasia in rats.

OBJECTIVES: Depletion of early growth response factor-1 (Egr-1) by a DNA enzyme, ED5, inhibits neointimal hyperplasia (NH) following vascular injury by an unknown mechanism. The aim of this study was to characterize the effects of ED5 in a rat carotid injury model in order to elucidate the mechanism by which ED5 inhibits NH. METHODS: ED5 was transfected into the arterial wall of Wistar rats using FuGENE6 transfection reagent following artery balloon injury. Hematoxylin and eosin staining, immunohistochemistry, real-time reverse transcription polymerase chain reaction and Western blotting analysis were used to characterize the response to ED5. RESULTS: NH decreased significantly in the ED5- plus FuGENE6-treated rats (p < 0.05) compared with the control groups, and this was accompanied by a reduced inflammatory response. Egr-1 mRNA and protein levels were significantly decreased in the ED5-treated group, as expected. The decrease in Egr-1 was accompanied by decreases in the mRNA and protein levels of PDGF-BB, Cyclin D1, CDK4, MCP-1, and ICAM-1 (p < 0.05). CONCLUSIONS: Transfection of the Egr-1-specific synthetic DNA enzyme ED5 significantly reduced NH after injury in rats, at least in part, as a result of decreased expression of downstream proliferative genes such as PDGF-BB, Cyclin D1, CDK4, and the inflammatory factors MCP-1 and ICAM-1.