RESUMO
OBJECTIVE: Long non-coding RNA growth arrest-specific 5 (lnc-GAS5) and its targets (microRNA [miR]-21 and miR-140) are involved in the development and progression of allergic rhinitis (AR). However, the correlation of lnc-GAS5 with miR-21 and miR-140 and their associations with disease risk, symptom severity, and Th1/Th2 cytokines in AR remain unclear. Thus, this study aimed to investigate this topic. METHODS: In total, 120 patients with AR and 60 controls were recruited. Nasal-mucosa tissues were collected from all participants. Lnc-GAS5, its targets (miR-21 and miR-140), interferon (IFN)-γ, interleukin (IL)-2, IL-4, and IL-10 were detected by reverse-transcription quantitative polymerase chain reaction. RESULTS: Lnc-GAS5 was elevated, while miR-21 and miR-140 was downregulated in AR patients than in controls (p < 0.001). In AR patients, lnc-GAS5 was negatively correlated with miR-21 (p < 0.001), miR-140 (p < 0.001), IFN-γ (p = 0.019), and IL-2 (p = 0.039) and positively correlated with IL-4 (p = 0.004) and IL-10 (p < 0.001), individual nasal symptom scores (INSSs) for itching, sneezing, and congestion (p < 0.05), and total nasal symptom score (TNSS) (p < 0.001). Moreover, miR-21 and miR-140 were negatively correlated with some INSSs, total TNSS score, and IL-10 and positively correlated with IFN-γ and IL-2 (p < 0.05). CONCLUSION: Lnc-GAS5 is negatively correlated with that of its targets (miR-21 and miR-140) in AR; meanwhile, lnc-GAS5, miR-21, and miR-140 are correlated with disease risk, symptom severity, and Th1/Th2 imbalance in AR, suggesting the potential of these biomarkers in the development and progression of AR.
Assuntos
MicroRNAs/genética , RNA Longo não Codificante/genética , Rinite Alérgica , Adulto , Biomarcadores , Citocinas/metabolismo , Feminino , Humanos , Masculino , MicroRNAs/metabolismo , RNA Longo não Codificante/metabolismo , Rinite Alérgica/epidemiologia , Rinite Alérgica/genética , Rinite Alérgica/metabolismo , Adulto JovemRESUMO
OBJECTIVE: To investigate the clinical efficacy of radiofrequency ablation in treating the inferior turbinates hypertrophic and allergic rhinitis after septoplasty or nasal polypectomy. METHOD: Ninety-one patients with nasal septum deviation or nasal polyp with inferior turbinates hypertrophic and(or) allergic rhinitis were underwent septoplasty or nasal polypectomy. Sixty-two cases of 91 patients with inferior turbinates hypertrophic or allergic rhinitis underwent radiofrequency ablation, 29 cases did not. The preoperative and postoperative nasal obstructions were investigated by visual analogue scale (VAS). Allergic rhinitis symptoms were assessed by 1997s Haikou standard. RESULT: The nasal obstruction and allergic symptom of 62 patients who underwent radiofrequency ablation were improved in 3 months postoperatively. VAS improved from (89.5+/-8.7) % to (26.2+/-8.7) % (left side) and (90.3+/-10.5) % to (32.1+/-10.3) % (right side) (P < 0.01). In control group: VAS improved from (90.5+/-8.0) % to (39.2+/-8.9) % (left side) and (89.9+/-11.5) % to (48.1+/-8.1) % (right side) (P <0.01). However, the mean of postoperative nasal obstruction VAS in radiofrequency ablation group is significantly higher than that in control group (P <0.01). CONCLUSION: Combination application of radiofrequency ablation in nasal operation to cure the inferior turbinates hypertrophic and allergic rhinitis is a safe, effective and less traumatic method.
