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1.
Talanta ; 278: 126402, 2024 Jun 24.
Artigo em Inglês | MEDLINE | ID: mdl-38924985

RESUMO

Simultaneous detection of multiple biomarker levels is essential to improve the accuracy of early diagnosis. Introducing capillary will simplify procedure, less time, and reduce reagent consumption for point-of-care testing of biomarkers. Here, we developed a portable and controllable smartphone-integrated fluorescence capillary imprinted sensing platform for the accuracy visual detection of Crohn's disease biomarkers (lysozyme, Fe3+) using single-excitation/double-signal detection. A novel controllable capillary coating strategy was developed by static gas-driven coating method for synthesis uniform fluorescence capillary imprinted sensor (Si-CD/g-CdTe@MIP capillary sensor). When Fe3+ and lysozyme were added, the fluorescence intensity of Si-CD/g-CdTe@MIP capillary sensor was quenched at 426 nm and enhanced at 546 nm, respectively. This Si-CD/g-CdTe@MIP capillary sensor has high sensitivity and selectivity for quantification lysozyme and Fe3+ simultaneously with the detection limit of 0.098 nM and 0.20 nM, respectively. In addition, the smartphone-integrated Si-CD/g-CdTe@MIP capillary sensor was applied for the intelligent detection of lysozyme and Fe3+, in which the detection limit was calculated as 0.32 nM and 0.65 nM. The smartphone-integrated visual Si-CD/g-CdTe@MIP capillary sensor realized ultrasensitive microanalysis (18 µL/time) of biomarkers in health man and Crohn 's patients, providing a novel strategy for early diagnosis of Crohn 's disease.

2.
Spectrochim Acta A Mol Biomol Spectrosc ; 314: 124221, 2024 Jun 05.
Artigo em Inglês | MEDLINE | ID: mdl-38569390

RESUMO

The toxicological effect between co-existed antibiotics and metal ions was dangerous to the ecological environment and public health. However, the rapid quantification tools with convenience, accuracy and low cost for the detection of multiple targets were still challenging. Herein, a portable tri-color ratiometric fluorescence paper sensor was constructed by coupling of blue carbon dots and fluorescence imprinted polymer for down/up conversion simultaneous detection of tetracycline and sulfamethazine. Interestingly, the cascade detection of aluminum ion was also realized based on the individual detection system of tetracycline without the assistance of complex coupling reagents. The detection limits of smartphone method for the visual detection of tetracycline, sulfamethazine and aluminum ion were calculated as 0.014 µM, 0.004 µM and 0.019 µM, respectively. The portable fluorescence paper sensor was applied for the visual detection of tetracycline, sulfamethazine and aluminum ion in actual samples successfully with satisfactory recoveries. With the advantages of rapidness, low cost, and portability, the developed portable fluorescence paper sensor provided a new strategy for the visual real-time detection of multiple targets.


Assuntos
Antibacterianos , Pontos Quânticos , Alumínio , Sulfametazina , Fluorescência , Tetraciclina , Carbono , Íons , Corantes Fluorescentes , Espectrometria de Fluorescência , Limite de Detecção
3.
Anal Chem ; 95(49): 18139-18148, 2023 Dec 12.
Artigo em Inglês | MEDLINE | ID: mdl-38013435

RESUMO

Herein, a dual-nanozyme cascade catalysis triemission fluorescence capillary imprinted sensor integrated with intelligent logic gates was constructed for simultaneous detection of 2,4-dichlorophenoxyacetic acid (2,4-DA) and 2,4-dichlorophenol (2,4-DCP). The novel nanozyme fluorescence organic framework (Bi, Co-MOF) was grafted on the surface of Fe3O4 modified with histidine to form a nanozyme composite (FBM) with dual-enzyme activity, which was imprinted with 2,4-DA to prepare a fluorescence molecularly imprinted polymer (FBM@MIP). Carbon dots (CDs) coupling with FBM@MIP (FBM@MIP/CDs) was inhaled into a capillary to construct a dual-nanozyme capillary imprinted sensor directly. The FBM@MIP/CDs capillary sensor realized to detect 2,4-DA and 2,4-DCP simultaneously within a linear concentration range of 1.0 × 10-12-1.2 × 10-9 M and 1.0 × 10-12-4.8 × 10-9 M with the detection limit of 0.75 and 0.68 pM, respectively. Interestingly, a smartphone-assisted portable capillary fluorescence intelligent sensing platform was developed that can detect 2,4-DA and 2,4-DCP visually without tedious operations such as soaking and drying. Combined with a smartphone, the linear relationships between RGB ratios and concentrations of 2,4-DA and 2,4-DCP were established with the detection limit of 0.93 and 0.81 pM, respectively. The integrated logic gates provided a promising way for intelligent sensing of multiple targets simultaneously, which provided a new strategy for ultrasensitive simultaneous detection of multiple pollutants with a microvolume (18 µL/time) in complex environments.

4.
Spectrochim Acta A Mol Biomol Spectrosc ; 302: 123103, 2023 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-37418902

RESUMO

Triclosan (TCS) is an endocrine disruptor, which has been widely used in daily chemicals, resulting in the potential risk to the ecosystem and human health. Herein, a smartphone-integrated bimetallic nanozyme triple-emission fluorescence capillary imprinted sensing system was developed for ultrasensitive and intelligent visual microanalysis of TCS. Carbon dots (CDs) and bimetallic organic framework (MOF-(Fe/Co)-NH2) were used as fluorescence sources to synthesize nanozyme fluorescence molecularly imprinted polymer (MOF-(Fe/Co)-NH2@CDs@NMIP), which oxidized o-phenylenediamine to 2,3-diaminophenazine (OPDox), resulting in the derivation of a new fluorescence peak at 556 nm. In the existence of TCS, the fluorescence of MOF-(Fe/Co)-NH2 at 450 nm was restored, the fluorescence of OPDox at 556 nm was suppressed, and the CDs fluorescence of at 686 nm remained constant. The color of triple-emission fluorescence imprinted sensor varied from yellow to pink to purple to blue. The response efficiency (F450/F556/F686) of this sensing platform based on the capillary waveguide effect demonstrated a significant linear relationship toward the concentration of TCS ranged from 1.0 × 10-12 to 1.5 × 10-10 M with the LOD of 8.0 × 10-13 M. Compared with dual-emission capillary fluorescence sensor, this sensing system has higher sensitivity and richer visual color. Combined with the smartphone-integrated portable sensing platform, the color of fluorescence was transformed into an RGB value to calculate TCS concentration with the LOD of 9.6 × 10-13 M, providing a novel method for intelligent visual microanalysis (18 µL/time) of environmental pollutants.


Assuntos
Impressão Molecular , Pontos Quânticos , Triclosan , Humanos , Pontos Quânticos/química , Limite de Detecção , Impressão Molecular/métodos , Ecossistema , Espectrometria de Fluorescência/métodos , Carbono/química , Corantes Fluorescentes/química
5.
Anal Chim Acta ; 1260: 341174, 2023 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-37121650

RESUMO

The abuse of multiple antibiotics and anti-inflammatory drugs can harm the ecological environment and human health. Herein, a smartphone-integrated tri-color fluorescence sensing platform based on acid-sensitive fluorescence imprinted polymers was proposed for dual-mode visual intelligent detection of ibuprofen (IP), chloramphenicol (CAP), and florfenicol (FF). In this research, the dual-mode of tri-color ratiometric fluorescence imprinted sensor (TC-FMIPs) was realized at different pH environments for the detection IP, CAP, and FF. The fluorescence peak at 551 nm of TC-FMIPs was quenched in the presence of IP solution and fluorescence peak at 687 nm was quenched in the presence of CAP phosphate buffer solution (PBS, pH 7.0), while the fluorescence peak at 433 nm kept stable. Interestingly, the TC-FMIPs has a peroxidase-like activity, in which a new fluorescence peak at 561 nm was quenched and the fluorescence peak at 433 nm increased gradually with the addition of FF solution in pH 4.0 PBS. The TC-FMIPs showed a low detection limit of 10 pM, 8.5 pM, and 5.5 nM for IP, CAP, and FF, respectively. Additionally, a smartphone was used to capture of fluorescence colors and read out the RGB values for intelligent detection of IP, CAP, and FF, in which the detection limit was calculated as 15 pM, 12 pM and 7 nM toward IP, CAP and FF, respectively. The smartphone-integrated tri-color fluorescence sensing platform was developed for dual-mode visual intelligent detection of IP, CAP and FF successfully, which provided a new strategy for multi-target detection in the complex environment.


Assuntos
Cloranfenicol , Impressão Molecular , Humanos , Ibuprofeno , Polímeros , Smartphone
6.
Artigo em Inglês | MEDLINE | ID: mdl-35137675

RESUMO

PM2.5-bound polycyclic aromatic hydrocarbons (PAHs) have been proved to be hazardous to health. Previous studies have focused on the distribution and sources of PAHs, whereas there is little knowledge of the damage to organs. Here we sought to investigate the pollution level and seasonal variation characteristics of PAHs in PM2.5 in Xi'an and assess the health risk, to establish a PAHs exposure model, and investigate the toxicological effects of PAHs on the respiratory and immune functions. A sub-chronic exposure model of PAHs was established by inhalation. The pathological changes of lung tissues were observed with a light microscope. Inflammatory reactions in alveolar lavage fluid were determined using the corresponding kit. The levels of interleukin-6 (IL-6) and interleukin-8 (IL-8) were detected with enzyme linked immunosorbent assay (ELISA) kit; the proliferation of lymphocytes in spleen was detected with methyl tetrazolium (MTT); DNA immune damage was determined with DNA gel electrophoresis. The results showed that (1) the total concentration of 16 PAHs ranged from 41.1 to 387 ng/m3, with a mean value of 170 ng/m3, and the concentration of PAHs in PM2.5 was higher in winter than in other seasons. (2) The sources of PAHs in the atmosphere of Xi'an urban area were mainly coal combustion, and the equivalent carcinogenic concentration of PAHs in PM2.5 was 3.9 ng/m3. (3) Foreign body granuloma formation and inflammatory cell damage were observed in the lungs of rats infected with toxin; the levels of reactive oxygen species (ROS) and mobile device assistant (MDA) increased while nitric oxide synthase (NOS) decreased with the increase of dose; the expression levels of IL-6 and IL-8 elevated with the increase of toxin dose, showing an obvious dose-effect relationship; the level of PAHs damage to cells showed a dose-effect relationship. Sub-chronic exposure to PAHs could cause sustained inflammatory injury to the organism. Measures should be taken to counter the problems of PAHs in PM2.5 in Xi'an and relevant health promotion strategies should be developed.


Assuntos
Poluentes Atmosféricos , Hidrocarbonetos Policíclicos Aromáticos , Animais , Ratos , Poluentes Atmosféricos/toxicidade , Poluentes Atmosféricos/análise , Monitoramento Ambiental/métodos , Estações do Ano , Interleucina-8 , Hidrocarbonetos Policíclicos Aromáticos/toxicidade , Hidrocarbonetos Policíclicos Aromáticos/análise , Interleucina-6/análise , Baço , Material Particulado/toxicidade , Material Particulado/análise , China , Medição de Risco
7.
Iran J Public Health ; 45(10): 1300-1308, 2016 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-27957436

RESUMO

BACKGROUND: College student community is the one with high risk of tuberculosis (TB). A systemic and standardized administration model for prevention and control of TB is significance in controlling TB spread in universities. Currently, the universities in China have not established the comprehensive and standardized administration system for TB prevention and control in college student community. METHODS: Firstly, the literature research and brainstorming method (n=13) were used to construct the clause and sub-clause pool for the administration of TB prevention and control within college student community in 2014. Secondly, a total of twenty experts in the field of TB prevention and control who are representatives of the east, west, south and north parts of China were selected and invited to participate the Delphi letter-inquiry. After two rounds of letter-inquiry, the opinions of the experts reached a consensus and the framework for the administration system was constructed. RESULTS: A framework for the administration system was constructed, which included 8 first class indexes, 26 second class indexes and 104 third class indexes. CONCLUSION: The results are highly scientific and reliable, which can be helpful for improving the systemic and standardized levels for the administration of TB prevention and control in universities in China and perhaps in other developing counties with high TB burden as well.

8.
BMC Public Health ; 14: 132, 2014 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-24507182

RESUMO

BACKGROUND: Tuberculosis (TB) remains a serious epidemic in China. In the past five years, the number of TB infections in high school students is rising and thus high school students are becoming a high risk group of TB. Parents of children with TB have to endure high psychological pressures from the disease itself, children's education, employment and life. The purpose of this study was to investigate the psychological pressure of parents with high school students suffering from TB. METHODS: A total of 22 parents who have been taking care of their children suffering from TB were interviewed and a framework approach was used to analyze the interviews. RESULTS: In our study, 21/22 parents had low levels of understanding about TB; 22/22 were under psychological stress; and 20/22 stated that their daily life was impacted on TB. CONCLUSIONS: Parents need to be given appropriate knowledge on TB and psychological counseling. Authorities should not only implement the therapeutic measures, but also focus on solving the psychological problems of patients and their families when a similar outbreak occurs.


Assuntos
Cuidadores/psicologia , Surtos de Doenças/estatística & dados numéricos , Pais/psicologia , Pesquisa Qualitativa , Tuberculose/epidemiologia , Tuberculose/psicologia , Adolescente , Adulto , Cuidadores/estatística & dados numéricos , China/epidemiologia , Efeitos Psicossociais da Doença , Feminino , Necessidades e Demandas de Serviços de Saúde , Humanos , Entrevistas como Assunto , Masculino , Instituições Acadêmicas , Estresse Psicológico
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