[Effects of silicon on exchange characteristics of H2O and CO2 in ginger leaves].

With solution culture, this paper studied the effects of silicon (Si) on the plant growth, Si content, photosynthetic rate, and transpiration rate of ginger (Zingiber officinale Rosc. ) cultivar, Laiwu big ginger. The results indicated that the biomass per plant and the leaf Si content were increased dramatically with increasing Si level in culture solution. In treatments T1 (1.0 mmol Si x L(-1)), T2 (1.5 mmol Si x L(-1)), and T3 (2.0 mmol Si x L(-1), the leaf Si content was increased 604.4%, 834.8%, and 1130.4%, and the biomass per plant was increased by 9.4%, 19.4%, and 22.8%, respectively, compared with the control. With increasing Si level, the leaf Mg2+ -AT-Pase and Ca2+ -ATPase activities, photosynthetic rate (Pn), and water use efficiency (WUE) increased, while the transpiration rate (Tr) decreased. At 11:00 o'clock, the Pn in treatments T1, T2, and T3 increased by 11.2%, 21.8%, and 28.2%, WUE increased by 23.1%, 55.9% and 54.8%, while Tr, decreased by 6.3%, 17.1% and 19.2%, respectively, compared with the control. Si also improved the leaf light saturation point (LSP), carboxylation efficiency (CE), and carotenoid content significantly, but had less effect on chlorophyll content. In our study, the suitable Si concentration for ginger was 1.5-2.0 mmol x L(-1).

Co-expression of heat shock protein 70 and glucose-regulated protein 94 in human gastric carcinoma cell line BGC-823.

AIM: To investigate the co-expression and significance of heat shock protein 70 (HSP70) and glucose-regulated protein 94 (grp94) in human gastric carcinoma cell line BGC-823. METHODS: The expression and localization of HSP70 and grp94 in human gastric carcinoma cell line BGC-823 were determined by immunocytochemistry and indirect immunofluorescence cytochemical staining. Flow cytometry was used to analyze the correlation between expression of HSP70, grp94 and cell cycle in BGC-823 cell line. RESULTS: Gastric cancer cell line BGC-823 expressed high level of HSP70 and grp94. The positive rate of HSP70 and grp94 was 84.9+/-4.94% and 79.6+/-5.16%, respectively. Both of them were stained in cell plasma. There was a significant difference compared with control group (1.9+/-0.94%, P<0.01). During the cell cycle, HSP70 and grp94 were continuously expressed in BGC-823. CONCLUSION: HSP70 and grp94 are highly expressed in human gastric carcinoma BGC-823 cells through the whole cell cycle. There is no relationship between expression of HSP70, grp94 and cell cycle.