RESUMO
PREMISE OF THE STUDY: Microsatellite markers were developed in an economically important plant, Siraitia grosvenorii, to evaluate its genetic diversity. METHODS AND RESULTS: Using the combined biotin capture method, 15 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from two to 12 across 98 individuals from cultivars and a wild population. The observed and expected heterozygosities ranged from 0.000 to 0.905 and from 0.000 to 0.845, respectively. CONCLUSIONS: These markers will facilitate the breeding and further study of the genetic diversity of S. grosvenorii.
Assuntos
Cucurbitaceae/genética , Primers do DNA , Repetições de Microssatélites , Polimorfismo Genético , Alelos , Produtos Agrícolas/genética , DNA de Plantas , Genética Populacional , Heterozigoto , Medicina Tradicional Chinesa , Plantas Medicinais/genéticaRESUMO
PREMISE OF THE STUDY: Microsatellite markers were developed in an endangered plant, Kmeria septentrionalis, to investigate its population genetic structure, gene flow, and mating systems. METHODS AND RESULTS: Using the combined biotin capture method, 14 microsatellite primer sets were isolated and characterized. All of these markers showed polymorphism, and the number of alleles per locus ranged from 3 to 15 across 60 individuals from two populations. The observed and expected heterozygosities ranged from 0.2692 to 0.9667 and from 0.3271 to 0.8881, respectively. CONCLUSIONS: These markers will facilitate further studies on the genetic diversity and mating systems of K. septentrionalis.
