Effects of carrier surface hydrophilic modification on sludge granulation: From sludge characteristics, extracellular polymeric substances, and microbial community.

Aerobic granular sludge (AGS) is a powerful biotechnological tool capable of treating multiple pollutants simultaneously. However, the granulation process and pollutant removal efficiency still need to be further improved. In this study, Fe2O3- and MnO2-surface-modified straw foam-based AGS (Fe2O3@SF-AGS and MnO2@SF-AGS), with an average particle size of 3 mm, were developed and evaluated. The results showed that surface modification reduced the hydrophobic groups of carriers, facilitating the attachment and proliferation of microorganisms. Notably, MnO2@SF-AGS showed excellent granulation performance, reaching a stable state about one week earlier than the unmodified SF-AGS. The polymeric substance content of MnO2@SF-AGS was found to be 1.28 times higher than that of the control group. Furthermore, the removal rates for NH4+-N, TN, and TP were enhanced by 27.28%, 12.8%, and 32.14%, respectively. The bacterial communities exhibited significant variations in response to different surface modifications of AGS, with genera such as Saprospiraceae, Terrimonas, and Ferruginibacter playing a crucial role in the formation of AGS and the removal of pollutants specifically in MnO2@SF-AGS. The charge transfer of metal ions of MnO2@SF promotes the granulation process and pollutant removal. These results highlight that MnO2@SF-AGS is an effective strategy for improving nitrogen and phosphorus removal efficiency from wastewater.

Biodegradation of p-hydroxybenzoic acid in Herbaspirillum aquaticum KLS-1 isolated from tailing soil: Characterization and molecular mechanism.

The wide distribution of p-hydroxybenzoic acid (PHBA) in the environments has attracted great concerns due to its potential risks to organisms. Bioremediation is considered a green way to remove PHBA from environment. Here, a new PHBA-degrading bacterium Herbaspirillum aquaticum KLS-1was isolated and its PHBA degradation mechanisms were fully evaluated. Results showed that strain KLS-1 could utilize PHBA as the sole carbon source and completely degrade 500 mg/L PHBA within 18 h. The optimal conditions for bacterial growth and PHBA degradation were pH values of 6.0-8.0, temperatures of 30 °C-35 °C, shaking speed of 180 rpm, Mg2+ concentration of 2.0 mM and Fe2+ concentration of 1.0 mM. Draft genome sequencing and functional gene annotations identified three operons (i.e., pobRA, pcaRHGBD and pcaRIJ) and several free genes possibly participating in PHBA degradation. The key genes pobA, ubiA, fadA, ligK and ubiG involved in the regulation of protocatechuate and ubiquinone (UQ) metabolisms were successfully amplified in strain KLS-1 at mRNA level. Our data suggested that PHBA could be degraded by strain KLS-1 via the protocatechuate ortho-/meta-cleavage pathway and UQ biosynthesis pathway. This study has provided a new PHBA-degrading bacterium for potential bioremediation of PHBA pollution.

Detecting Pb2+by a 'turn-on' fluorescence sensor based on DNA functionalized magnetic nanocomposites.

Sensitive and selective detection of the lead ion (Pb2+) plays an important role in terms of both human health and environmental protection, as the heavy metal is fairly ubiquitous and highly toxic. The highly stable fluorescence biosensor is composed of Fe3O4@TiO2core-shell nanocomposites, functionalized with a carboxyl fluorescein labeled DNA. The morphology, physical and chemical properties of the sensing nanomaterials were studied by transmission electron microscopy, FT-IR spectroscopy (FT-IR), x-ray powder diffraction and vibrating sample magnetometer. UV-visible and fluorescence spectroscopy were used to characterize the fluorescein functionalized magnetic nanoparticles. The performance of Pb2+detection displayed an excellent linearity (R2 = 0.995) in the range of 10-10to 5 × 10-9ppm with a detection limit of 10-10ppm, based on the optimization of the fabrication process and aptamers' specification. The fluorescence biosensor has an accurate response, excellent recoveries and high adsorbent capacities. It was successfully applied for the determination of Pb2+in contaminated water and serum samples; the detection of limit in both media were 10-10ppm. These features ensure the potential use of aptamer functionalized magnetic nanocomposites as a new class of non-toxic biocompatible sensors for biological and environmental applications.

Zirconia supported gold-palladium nanocatalyst for NAD(P)H regeneration via two-step mechanism.

The regeneration cycle of expensive cofactor, NAD(P)H, is of paramount importance for the bio-catalyzed redox reactions. Here a ZrO2supported bimetallic nanocatalyst of gold-palladium (Au-Pd/ZrO2) was prepared to catalyze the regeneration of NAD(P)H without using electron mediators and extra energy input. Over 98% of regeneration efficiency can be achieved catlyzed by Au-Pd/ZrO2using TEOA as the electron donor. Mechanism study showed that the regeneration of NAD(P)H took place through a two-step process: Au-Pd/ZrO2nanocatalyst first catalyzed the oxidation of triethanolamine (TEOA) to glycolaldehyde (GA), then the generated GA induced the non-catalytic reducing of NAD(P)+to NAD(P)H under an alkaline environment maintained by TEOA. This two-step mechanism enables the decoupling of the regeneration of NAD(P)H in space and time into a catalytic oxidation and non-catalytic reducing cascade process which has been further verified using a variety of electron donors. The application significance of this procedure is further demonstrated both by the favorable stability of Au-Pd/ZrO2nanocatalyst in 5 successive cycles preserving over 90% of its original activity, and by the excellent performance of the regenerated NADH as the cofactor in the catalytic hydrogenation of acetaldehyde using an ethanol dehydrogenase.

A reusable chitosan/TiO2@g-C3N4 nanocomposite membrane for photocatalytic removal of multiple toxic water pollutants under visible light.

Photocatalysis has been proved to be a promising approach in wastewater purification. However, it is hard to recycle powdery photocatalysts from wastewater in industry, but immobilizing them using larger materials can overcome this drawback. For that reason, TiO2@g-C3N4 was embedded into chitosan to synthesize a highly reusable and visible-light-driven chitosan/TiO2@g-C3N4 nanocomposite membrane (CTGM). CTGM showed enhanced photoactivity and the photocatalytic efficiencies of the toxic water pollutants methyl orange (M.O.), rhodamine B (Rh.B), chromium (VI) (Cr (VI)), 2,4-dichlorophenol (2,4-DCP) and atrazine (ATZ) were more than 90% under visible light at ambient conditions. Significantly, CTGM was easy to recycle and showed excellent reusability: there was no decrease in the photocatalytic decolorization efficiency of Rh.B throughout 10 cycles. A continuous-flow photocatalysis system was set up and 90% of Rh.B was effectively decolorized. A simple approach was developed to prepare a novel, effective and visible-light-driven membrane that was easy to reuse, and a feasible photocatalysis continuous-flow system was designed to be a reference for wastewater treatment in industry.

Fluoride-capped nanoceria as a highly efficient oxidase-mimicking nanozyme: inhibiting product adsorption and increasing oxygen vacancies.

Nanozymes aim to mimic enzyme activities using nanomaterials. Nanoceria (CeO2 nanoparticles) is an important model nanozyme for its rich redox chemistry. In particular, its oxidase-like activity allows oxidation reactions without the need of unstable and toxic H2O2. Fluoride can significantly improve its oxidase-like activity, and this work aims to understand the mechanism of fluoride-promoted catalysis. First, fluoride can adsorb on CeO2 tighter than other halides, but not as strong as phosphate as characterized by isothermal titration calorimetry (ITC). FT-IR spectroscopy indicates adsorption of fluoride likely via exchange with surface hydroxide groups. Fluoride capping inverses the surface charge of CeO2, facilitating desorption of the ABTS oxidation product, significantly increasing the turnover number. The Raman, EPR and XPS spectroscopy results demonstrate that the concentration of Ce3+ and the accompanying oxygen vacancy significantly increased upon adding F-, which can explain the enhanced catalytic activity. Finally, the electron transfer properties of fluoride-capped CeO2 were more efficient than that of the bare CeO2 as determined by a direct electrochemical measurement on a glass carbon electrode. This study has provided new insight into nanoceria, and can also further confirm the role of nanoceria as a model for engineering the surface of nanozymes.

Preparation of chitosan-TiO2 composite film with efficient antimicrobial activities under visible light for food packaging applications.

By incorporation of TiO2 nano-powder in chitosan, a chitosan-TiO2 composite film was prepared with efficient antimicrobial activity against food-borne pathogenic microbes and expected to be a promising food packaging material. Scanning electron microscopy analysis showed that the TiO2 nano-powder was successfully and uniformly dispersed into the chitosan matrix. TiO2 addition led to enhanced hydrophilicity, to better mechanical properties, and to decreased light transmittance in visible light region of the composite film. The chitosan-TiO2 film possessed efficient antimicrobial activity against four tested strains, i.e. Escherichia coli, Staphylococcus aureus, Candida albicans, and Aspergillus niger with 100% sterilization in 12h. It moreover provoked the leakage of cellular substances through damaged membrane. The prepared chitosan-TiO2 film was tested for packaging red grapes to prevent microbial infection and extend their shelf life. Results were positive, stressing the potential of the novel bio-nano composite film for food packaging applications.

Effect of moderate salinity stress treatment on the stimulation of proline uptake and growth in Escherichia coli CSH4 and its mutants under high salinity.

Activity of proline uptake in Escherichia coli CSH4 was inhibited in the presence of 1 M NaCl, while it was recovered if the cells were incubated at 30 degrees C for 1 h in a moderate salinity stress (MSS) solution which consists of Davis minimal medium with 5 mM proline and 0.5 M NaCl. Then, an attempt was made to examine whether MSS treatment is also effective on the activity restoration of proline uptake and growth under high salinity for E. coli CSH4 mutants with different combinations of proP, putA, putP, and proU which are related to the transport and metabolization of proline. After MSS treatment, proline uptake was vigorously occurred for the mutants with proline transporter gene proP but not for its deficient ones. For the expression of proline uptake activities of these mutant strains after MSS treatment, PO(4)(3-) in MSS solution is more important than K(+). No growth of strain CSH4 and its mutants without MSS treatment was observed, when cultured in high osmotic medium G (0.8 M NaCl) consisting of 1 mM glycine betaine and Davis minimal medium without potassium phosphate supplemented. After MSS treatment, however, mutant strains lacking proP showed sufficient growth in medium G. Cell growth of proP(+) strains was recognized if MSS treatment was performed in the absence of proline. In conclusion, growth of mutant strains under high-salinity medium G depended on their amount of proline accumulated during MSS treatment, in which K(+) and PO(4)(3-) might play a key role to guarantee their sufficient growth.

Efficient cyclic system to yield ectoine using Brevibacterium sp. JCM 6894 subjected to osmotic downshock.

Brevibacterium sp. JCM 6894 cells grown in the presence of 1.5-2.5 M NaCl for 24 h at 30 degrees C were subjected to the osmotic downshock. Downshocked cells after ectoine release were grown for further 24 h in the fresh medium with same salinity as before shock. When this cyclic system was applied to the strain JCM 6894, the amount of ectoine in the cells increased with an increase of incubation time, which indicates that the cells manipulated by the present conditions were enough active to survive and synthesize ectoine after several times of osmotic downshock. In the presence of 2 M NaCl, the highest yield of ectoine released was achieved in this cyclic system, more than 2.4 g/L during 7 days of incubation. (1)H and (13)C-NMR analyses of solutes released from the cells by the osmotic downshock showed the presence of only ectoine with high purity. Release of ectoine from the cells was carried out within 5 min and its rates were increased by the dilution in the downshock treatment. For the convenience of operations, non-sterilized medium containing 2 M NaCl was examined for the cell growth in the present system, in which almost same level of ectoine yield, release rates, and cell viability were observed as those of sterilized medium.