RESUMO
The stems of Cinnamomum reticulatum Hay (Lauraceae) were extracted with hexane and chloroform successively. A series of new esters, including a mixture of 4-hydroxy-3-methoxyphenethyl derivatives, along with two butanolides, isoobtusilactone A and obtusilactone A, two amides, N-trans-feruloylmethoxytyramine and N-cis-feruloyl-methoxytyramine, three benzenoids, p-hydroxybenzoic acid, syringic acid and vanillic acid, one lignan, (+)-syringaresinol and one steroid, beta-sitostenone, were isolated. The structures of the new esters were elucidated by chemical and physical evidence.
Assuntos
Cinnamomum/química , Ésteres/química , Estrutura MolecularRESUMO
(-)-N-Formylanonaine (1), (-)-oliveroline (2), (+)-nornuciferine (3), lysicamine (4), (+)-cyperone (5), (+)-epi-yangambin (6), ficaprenol-10 (7), pheophytin a (8), aristophyll C (9) and michephyll A (10) were isolated from the leaves of Michelia alba DC (Magnoliaceae). Among them, 10 is a new compound. The structures of these compounds were characterised and identified by spectral analyses. We have also presented the antioxidation activity of 10.
Assuntos
Alcaloides/isolamento & purificação , Antioxidantes/isolamento & purificação , Clorofila/análogos & derivados , Magnoliaceae/química , Extratos Vegetais/isolamento & purificação , Terpenos/isolamento & purificação , Alcaloides/química , Antioxidantes/química , Aporfinas/química , Aporfinas/isolamento & purificação , Benzotiazóis/metabolismo , Clorofila/química , Clorofila/isolamento & purificação , Ressonância Magnética Nuclear Biomolecular , Feofitinas/química , Feofitinas/isolamento & purificação , Extratos Vegetais/química , Folhas de Planta/química , Espectrometria de Massas de Bombardeamento Rápido de Átomos , Espectrofotometria Infravermelho , Espectrofotometria Ultravioleta , Ácidos Sulfônicos/metabolismo , Terpenos/químicaRESUMO
Subamone (1), a novel Cinnamomum monoterpenoid, has been isolated from Cinnamomum subavenium (Lauraceae), and its structure was determined on the basis of spectroscopic analysis. Subamone's cytotoxic activities were evaluated against A549 (human lung cancer cell), and DU-145 and LNCaP (human prostate cancer cell lines) and its cytotoxicity was found to be significantly against LNCaP rather than DU-145 and A549 cancer cell lines.
Assuntos
Cinnamomum/química , Monoterpenos/farmacologia , Linhagem Celular Tumoral , Cromatografia Líquida , Humanos , Monoterpenos/química , Espectrometria de Massas por Ionização por Electrospray , Espectrofotometria Infravermelho , Espectrofotometria UltravioletaRESUMO
Two new butanolides, subamolide D (1) and subamolide E (2), and a new secobutanolide, secosubamolide A (3), along with 21 known compounds were isolated from the leaves of Cinnamomum subavenium. The structures of 1-3 were determined by spectroscopic analysis. Propidium iodide staining and cytometry analysis were used to evaluate the cell cycle progression of the treated SW480 cells and it was found that 1 and 2 caused DNA damage in a dose- and time-dependent manner.
Assuntos
4-Butirolactona , Antineoplásicos Fitogênicos , Cinnamomum/química , Plantas Medicinais/química , 4-Butirolactona/análogos & derivados , 4-Butirolactona/química , 4-Butirolactona/isolamento & purificação , 4-Butirolactona/farmacologia , Antineoplásicos Fitogênicos/química , Antineoplásicos Fitogênicos/isolamento & purificação , Antineoplásicos Fitogênicos/farmacologia , Ciclo Celular/efeitos dos fármacos , Dano ao DNA , Ensaios de Seleção de Medicamentos Antitumorais , Humanos , Estrutura Molecular , Folhas de Planta/química , TaiwanRESUMO
The aim of this study was to evaluate the cytotoxic effects of three new butanolides, subamolides A - C (1-3), and a new secobutanolide, secosubamolide (4), on the human colorectal cancer cell line SW480. Compounds 1-4 are new and were isolated from the stems of Cinnamomum subavenium, along with 17 known compounds. The structures of 1-4 were determined by spectroscopic analysis. Propidium iodide staining and flow cytometry were used to evaluate DNA damage of the treated SW480 cells, and it was found that 1-4 caused DNA damage in a dose-dependent manner after 24 h of treatment.
