Research Progress of Yunnan Unexplained Sudden Death / 法医学杂志

Yunnan unexplained sudden death (YUSD) has obvious spatial and temporal aggregations.With the features of sudden onset and rapid death,its causes remain unclear.However,the onset of YUSD is related to the geological and climatic conditions in specific range of altitude of incidence area,which is also influenced by the existed susceptibility gene loci or several multiple mutations in SNP loci,long-term fatigue,low dietary nutrition,trace element deficiency,poor living condition and hygienic habit,and infection by etiologic microorganism or virus among the residents live in the incidence area of YUSD.Under the continuous influence of factors above,the crowd of incidence area finally occurred unexplained sudden death that prominently shown by myocardial injury.Improvements of public health administration,living conditions,villagers' health,living habits and enhancement of indicator measurement of myocardial enzyme and electrocardiogram for the residents in the incidence area of YUSD are effective measures for prevention of YUSD.Timely identification of cause of death and in-depth genetic research are important ways to explore the causes of YUSD,enhance the effectiveness of treatment and reduce the death rate.

[Study on structural properties of biochar under different materials and carbonized by FTIR].

Infrared spectroscopy (IR) is an important means of seeing the characteristics of the structural properties. Fourier transform infrared spectroscopy (FTIR) was applied to analyze the structural properties of biochar from different materials with different methods. The results showed that: the biochars have IR absorption peaks of hydroxyls group, aromatic group and containing organic group with the activated? charcoal, but in other absorption peaks, with a significant difference. The high temperature can make -OH, -CH3, -CH2-, -C=O to be associated or loss, and promotes the formation of aromatic groups during Carbonization of corn straw. At the different carbonization mode, the heating and microwave carbonization, has a carbonize mechanism of biochar, heating method may make -OH in alcohol and phenol to combinative with each other or loss, and to form benzene ring group and an aromatic group, Aromatic group in microwave method was so preventing to participate in the hot reaction, to form the more benzene substances. These results show that the Infrared spectrum can well analysis the structural characteristics of biochar, and showed that it comprises -OH, the aromatic group and other active groups.

Cytogenetic analysis of human bone marrow-derived mesenchymal stem cells passaged in vitro.

This study concerns the cytogenetic stability of in vitro human bone marrow-derived mesenchymal stem cells (MSCs) in primary culture and after passaging. Bone marrow samples were collected from seven brain malfunction patients involved in autologous MSC transplantation trials. Chromosome preparations from primary MSC cultures and after 3 passages were analyzed by conventional staining and G-banding techniques. All MSCs showed normal diploid karyotypes, 46 XY or 46 XX, without aneuploidy or polyploidy; chromosome structural abnormalities were not detected. The results indicate that the in vitro cultured MSCs retained normal cytogenetics before being transplanted back into the patients.