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J Agric Food Chem ; 65(2): 463-472, 2017 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-27997188

RESUMO

Soybean (Glycine max L. Merrill) is one of eight major allergenic foods with endogenous proteins identified as allergens. To better understand the natural variability of five soybean allergens (Gly m 4, Gly m 5, Gly m 6, Gly m Bd 28k, and Gly m Bd 30k), validated enzyme-linked immunosorbent assays (ELISAs) were developed. These ELISAs measured allergens in 604 soybean samples collected from locations in North and South America over five growing seasons (2009-2013/2014) and including 37 conventional varieties. Levels of these five allergens varied 5-19-fold. Multivariate statistical analyses and pairwise comparisons show that environmental factors have a larger effect on allergen levels than genetic factors. Therefore, from year to year, consumers are exposed to highly variable levels of allergens in soy-based foods, bringing into question whether quantitative comparison of endogenous allergen levels of new genetically modified soybean adds meaningful information to their overall safety risk assessment.


Assuntos
Alérgenos/análise , Glycine max/química , Proteínas de Soja/análise , Antígenos de Plantas/análise , Antígenos de Plantas/imunologia , Ensaio de Imunoadsorção Enzimática , Hipersensibilidade Alimentar , Variação Genética , Globulinas/análise , Globulinas/imunologia , Glicoproteínas , Análise Multivariada , América do Norte , Reprodutibilidade dos Testes , Proteínas de Armazenamento de Sementes/análise , Proteínas de Armazenamento de Sementes/imunologia , Sementes/química , América do Sul , Proteínas de Soja/genética , Proteínas de Soja/imunologia , Glycine max/genética , Glycine max/imunologia
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