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1.
Sensors (Basel) ; 24(9)2024 Apr 28.
Artigo em Inglês | MEDLINE | ID: mdl-38732921

RESUMO

In the context of construction and demolition waste exacerbating environmental pollution, the lack of recycling technology has hindered the green development of the industry. Previous studies have explored robot-based automated recycling methods, but their efficiency is limited by movement speed and detection range, so there is an urgent need to integrate drones into the recycling field to improve construction waste management efficiency. Preliminary investigations have shown that previous construction waste recognition techniques are ineffective when applied to UAVs and also lack a method to accurately convert waste locations in images to actual coordinates. Therefore, this study proposes a new method for autonomously labeling the location of construction waste using UAVs. Using images captured by UAVs, we compiled an image dataset and proposed a high-precision, long-range construction waste recognition algorithm. In addition, we proposed a method to convert the pixel positions of targets to actual positions. Finally, the study verified the effectiveness of the proposed method through experiments. Experimental results demonstrated that the approach proposed in this study enhanced the discernibility of computer vision algorithms towards small targets and high-frequency details within images. In a construction waste localization task using drones, involving high-resolution image recognition, the accuracy and recall were significantly improved by about 2% at speeds of up to 28 fps. The results of this study can guarantee the efficient application of drones to construction sites.

2.
ACS Appl Mater Interfaces ; 11(30): 26994-27003, 2019 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-31290644

RESUMO

Despite layered LiNixCoyMnzO2 having drawn much attention for their high capacity and high energy density, they still endure strong capacity decay upon prolonged cycling and high C-rates, primarily due to sluggish Li+ and charge-transfer kinetics and detrimental parasitic reactions with the electrolyte. To address these issues, application of a surface-coating layer made of V2O5/LiV3O8 on LiNi0.4Co0.2Mn0.4O2 (V-NCM) is pursued. Benefiting from the ionic conductivity of LiV3O8 and the electronic conductivity of V2O5, resulting in both enhanced Li+ diffusion and charge-transfer kinetics, the coated material offers significantly improved C-rate capability. Additionally, better long-term cycling performance is achieved mostly due to the mitigated parasitic reactions at the electrode/electrolyte interface that result in lower structural degradation. As a result, Li/V-NCM cells deliver over 100 mA h g-1 capacity at 10 C and also achieve 86.1% (2 C) and 94.1% (10 C) capacity retention after 200 cycles. These V-NCM cells operate quite stably even at elevated temperature, that is, 40 and 60 °C. The coating strategy herein reported may also be useful to enhance the cycling stability and C-rate capability of other layered cathode materials.

4.
Am J Physiol Lung Cell Mol Physiol ; 312(5): L741-L747, 2017 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-28213468

RESUMO

Idiopathic pulmonary fibrosis (IPF) is a progressive disease that causes unremitting deposition of extracellular matrix proteins, thus resulting in distortion of the pulmonary architecture and impaired gas exchange. Associated with high morbidity and mortality, IPF is generally refractory to current pharmacological therapies. Lefty A, a potent inhibitor of transforming growth factor-ß signaling, has been shown to have promising antifibrotic ability in vitro for the treatment of renal fibrosis and other potential organ fibroses. Here, we determined whether Lefty A can attenuate bleomycin (BLM)-induced pulmonary fibrosis in vivo based on a novel therapeutic strategy where human embryonic kidney 293 (HEK293) cells are genetically engineered with the Lefty A-associated GFP gene. The engineered HEK293 cells were encapsulated in alginate microcapsules and then subcutaneously implanted in ICR mice that had 1 wk earlier been intratracheally administered BLM to induce pulmonary fibrosis. The severity of fibrosis in lung tissue was assessed using pathological morphology and collagen expression to examine the effect of Lefty A released from the microencapsulated cells. The engineered HEK293 cells with Lefty A significantly reduced the expression of connective tissue growth factor and collagen type I mRNA, lessened the morphological fibrotic effects induced by BLM, and increased the expression of matrix metalloproteinase-9. This illustrates that engineered HEK293 cells with Lefty A can attenuate pulmonary fibrosis in vivo, thus providing a novel method to treat human pulmonary fibrotic disease and other organ fibroses.


Assuntos
Engenharia Celular , Composição de Medicamentos , Fatores de Determinação Direita-Esquerda/metabolismo , Fibrose Pulmonar/patologia , Fibrose Pulmonar/terapia , Animais , Colágeno/metabolismo , Fator de Crescimento do Tecido Conjuntivo/metabolismo , Vetores Genéticos/metabolismo , Células HEK293 , Humanos , Imuno-Histoquímica , Metaloproteinase 2 da Matriz/metabolismo , Metaloproteinase 9 da Matriz/metabolismo , Camundongos Endogâmicos ICR , Microesferas , Retroviridae/metabolismo
5.
ChemSusChem ; 9(19): 2824-2831, 2016 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-27572550

RESUMO

BiVO4 has been identified as one of the excellent visible light responsive photoanodes for use in photoelectrochemical (PEC) water splitting. However, pristine BiVO4 usually exhibits relative low photocatalytic properties owing to insufficient charge separation and transport characteristics. Although the marginal n-type doping of higher valence ions can obviously raise the photocurrent value, it by no means improves the PEC stability. In this work, we successfully enhanced the PEC stability of BiVO4 by doping Fe ions in substitution of Bi. Density functional theory calculations have illustrated that Fe-doping would result in an impurity band in the forbidden gap, and thus narrow its energy gap. More importantly, Fe-doping can synergize with other means to further improve the PEC activities of BiVO4 . Therefore, we fabricated a nanoporous Fe/W co-doped BiVO4 photoelectrode, and then loaded the metal-organic framework (MOF) MIL-100(Fe) as cocatalyst to further promote the separation of charge carriers. To the best of our knowledge, MOFs have not yet been utilized as a cocatalyst to facilitate the charge separation, which could increase the photocurrent density of Fe/W co-doped BiVO4 .


Assuntos
Bismuto/química , Eletrodos , Ferro/química , Tungstênio/química , Vanadatos/química , Catálise , Técnicas Eletroquímicas , Microscopia Eletrônica de Varredura , Processos Fotoquímicos , Espectrofotometria Ultravioleta
6.
Plant Sci ; 250: 30-39, 2016 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27457981

RESUMO

NAC membrane-bound transcription factors (NTM1-like, NTL proteins) participate in the regulation of plant development and the abiotic stress response. While their function has been thoroughly explored in Arabidopsis thaliana, this is not the case in maize. Seven ZmNTL genes were identified by an in silico scan of relevant genome sequence. All seven included a NAC domain at their N terminus, and an α-helical membrane-bound structure domain in their C terminal region. Based on their gene structure and content of conserved motifs, the seven sequences were distributed into four clades. Six of the seven ZmNTLs were associated with the plasma membrane, and the remaining one with the endoplasmic reticulum. ZmNTL2-7 were more strongly transcribed in the stem than in either the leaf or root, while ZmNTL1 transcript abundance was highest in the leaf. When the plants were exposed to either abscisic acid or hydrogen peroxide treatment, all seven genes were up-regulated in the root and stem and down-regulated in the leaf. The heterologous expression of ZmNTL1-ΔTM, 2-ΔTM and 5-ΔTM in A. thaliana reduced the level of sensitivity of the plant to hydrogen peroxide.


Assuntos
Regulação da Expressão Gênica de Plantas , Genoma de Planta , Proteínas de Membrana Transportadoras/genética , Proteínas de Plantas/genética , Zea mays/genética , Sequência de Aminoácidos , Arabidopsis/genética , Arabidopsis/metabolismo , Proteínas de Membrana Transportadoras/química , Proteínas de Membrana Transportadoras/metabolismo , Especificidade de Órgãos , Filogenia , Proteínas de Plantas/química , Proteínas de Plantas/metabolismo , Plantas Geneticamente Modificadas/genética , Plantas Geneticamente Modificadas/metabolismo , Alinhamento de Sequência , Estresse Fisiológico , Zea mays/metabolismo
7.
Theor Appl Genet ; 126(6): 1545-53, 2013 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-23494393

RESUMO

Phosphorus (P) is one of the essential macronutrients for plant growth and development. Grain yield is the primary trait of interest in maize breeding programs. Maize grain yield and yield-related traits are seriously affected by P deficiency. Kernel number per row (KN), as one of the major components of grain yield, has attracted the attention of more and more breeders. In our previous study, one major QTL (named qKN), controlling KN under different P regimes was mapped to the interval between molecular markers bnlg1360 and umc1645 on chromosome 10 using a F 2:3 population derived from the cross between maize inbreds 178 and 5,003 (107). In order to understand its genetic basis, we developed a population of near isogenic lines (NILs) and two P regimes were used to fine map and characterize qKN. The QTL qKN was finally localized in a region of ~480 kb. A single qKN allele of inbred 178 increased KN by 6.08-10.76 % in the 5,003 (107) background; qKN acted in a partially dominant manner. Our results will be instrumental for the future identification and isolation of the candidate gene underlying qKN. The tightly linked molecular markers that we developed for qKN will be useful in maize breeding programs for improving KN applying the marker-assisted selection.


Assuntos
Locos de Características Quantitativas/genética , Sementes/crescimento & desenvolvimento , Sementes/genética , Zea mays/genética , Agricultura/métodos , Cruzamento/métodos , Mapeamento Cromossômico , Fósforo/farmacologia , Sementes/efeitos dos fármacos
8.
Phys Rev E Stat Nonlin Soft Matter Phys ; 76(6 Pt 2): 066709, 2007 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18233945

RESUMO

Towards getting the full-scale solutions to particle-laden flows, a multidirect forcing technique and immersed boundary method are proposed in the present work. The immersed solid boundary is represented by Lagrangian points and the no-slip condition is efficiently satisfied by exerting multidirect forcing. The hydrodynamic interactions between the stationary or moving solid boundary and the Newtonian fluid are able to be accurately described. This method is simple but efficient which is validated by simulating the flows around a stationary circular disc at different Reynolds numbers and the free sedimentation of a particle. The predicted results agree well with previous experimental and numerical data. When applying this method to study particle sedimentation near a vertical wall, the rotation shifting phenomenon is observed besides the anomalous rolling and the lateral migration.

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