Defense against Paramecium predation via long filament morphology favors the survival of Raphidiopsis raciborskii populations.

Raphidiopsis blooms are notorious for cyanotoxin formation and strong invasiveness, threatening the stability of aquatic ecosystems and human health. The protozoa Paramecium can potentially serve as an organism for controlling Raphidiopsis blooms owing to its grazing effect. However, the grazing ability of Paramecium is largely determined by the size of the prey, and the population of Raphidiopsis consists of filaments of varying lengths and sizes. The selective grazing behavior of Paramecium toward short-length or small-sized filaments in the Raphidiopsis population, as opposed to long filaments, remains unclear. Therefore, in this study, we co-cultured the predator Paramecium sp. with different initial abundances and the prey Raphidiopsis raciborskii to explore this knowledge gap. Our results suggested that: (1) the population of R. raciborskii declined under the selective grazing effect of Paramecium sp. on short filaments, whereas R. raciborskii with long filaments survived; (2) the growth of Paramecium sp. feeding on the same abundance of R. raciborskii was reduced at higher initial abundances, whereas its carrying capacity exhibited an opposite trend; (3) under ingestion by Paramecium sp., the morphology of R. raciborskii developed in the direction of becoming larger, and higher initial abundances of Paramecium sp. intensified this process; (4) increasing initial abundance of Paramecium sp. aggravated the decline of R. raciborskii photosynthetic activity. Therefore, the grazing effect of Paramecium sp. on R. raciborskii mainly affects filaments of short length or small size. Collectively, these results clarify the inter-species interaction between the protozoa Paramecium and filamentous cyanobacteria Raphidiopsis, including population dynamics and morphological and physiological changes in the predator and prey. Such insights into the interactions between Paramecium and R. raciborskii may have implications for the biological control of blooms caused by filamentous cyanobacteria.

Long-term acclimation to warming improves the adaptive ability of Microcystis aeruginosa to high temperature: Based on growth, photosynthetic activity, and microcystin production.

Gradually warming of water bodies caused by climate change is expected to intensify the expansion of Microcystis blooms causing a series of severe problems in waters. However, most predictions about global warming further promoting the dominance of Microcystis are dependent on the strains only experiencing short-term acclimation to high temperature. It still remains unknown whether long-term warming acclimation improves the adaptive ability of Microcystis to high temperature. The present study used Microcysits aeruginosa maintained at 25 °C, short- and long -term acclimated at 30 °C to explore the above knowledge gaps. The results showed that: (1) The growth rate of long-term warming acclimated M. aeruginosa was significantly enhanced, compared with those of low temperature cultured and short-term warming acclimated ones; (2) A faster decline rate of photosynthetic activity during growth phase and a higher ultimately stable photosynthetic activity during stationary phase of M. aeruginosa were caused by longer warming acclimation time; (3) high temperature reduced the microcystin production of long-term warming acclimated M. aeruginosa compared to that of low temperature cultured M. aeruginosa; (4) Warming acclimation time improved the driving effect of photosynthetic activity on the growth of M. aeruginosa but decreased the restriction ability of growth state to microcystin production of M. aeruginosa at high temperature; (5) Compared to low temperature cultured M. aeruginosa, high temperature improved the driving effect of photosynthetic activity on the growth of long-term warming acclimated M. aeruginosa, but decreased the sensitivity of photosynthetic activities to environmental resources and the regulative ability of microcystin production to photosynthetic activity. These findings indicated that long-term warming acclimation enhanced M. aeruginosa adaptive ability to high temperature and demonstrated the necessity of applying long-term warming acclimated strains in the future studies about the impact of global warming on cyanobacteria.

Adverse role of colonial morphology and favorable function of microcystins for Microcystis to compete with Scenedesmus.

In eutrophic freshwaters, Microcystis usually becomes dominant in phytoplankton communities due to the synergistic effects of its special eco-physiological traits and environmental factors. Colonial morphology can protect Microcystis from zooplankton grazing, which indirectly favors Microcystis to outcompete other phytoplankton, although the colonial form is not conducive to the absorption of nutrients. Moreover, unicellular Microcystis usually has competitive advantages over other phytoplankton due to its efficient absorption capacity for nutrients and releasing microcystins. However, the consequence of direct competition between toxic colonial Microcystis and green algae without external grazing pressure still remained unknown. In this study, the competition between toxic colonial Microcystis aeruginosa and a common green alga Scenedesmus obliquus was explored. Results showed that: (1) colonial M. aeruginosa had a higher requirement for key macro-nutrient phosphorus than S. obliquus, and thus its population declined and was replaced by S. obliquus eventually; (2) microcystins released by colonial M. aeruginosa inhibited the photosynthetic activity and growth of S. obliquus at early stage of the competition; (3) the photosynthetic potential of colonial M. aeruginosa was stimulated in response to the competitive stress from S. obliquus, although the population of colonial M. aeruginosa declined eventually; (4) microcystin production of colonial M. aeruginosa was enhanced by phosphorus limitation due to S. obliquus competition and was positively related to photosynthetic potential of colonial M. aeruginosa. These results indicated that, in the absence of complex natural environment, colonial Microcystis cannot outcompete Scenedesmus in a pure competition, although microcystins can play a favorable role in the competition, which clarified the opposite role of colonies and microcystins in the competition of colonial Microcystis against other phytoplankton.

Cyanobacterial dominance and succession: Factors, mechanisms, predictions, and managements.

Eutrophication of natural water bodies worldwide has led to cyanobacteria becoming the dominant species in phytoplankton communities, causing serious harm environmentally and economically. Cyanobacterial succession makes effective treatment of cyanobacterial blooms a challenge. Although there are many studies about cyanobacterial dominance and succession, it is still lack of relevant review summarizing the advances on this topic. To control cyanobacterial blooms and manage water quality effectively, we conducted a critical review and drew the following conclusions: (1) cyanobacterial dominance and succession occur from spring to summer, with changes of multiple environmental factors dominated by temperature and nutrients conditions; (2) the cyanobacterial dominance and succession are inherently attributed to the distinctive traits of cyanobacteria including colony formation, gas vesicles, toxin release, and nitrogen fixation; (3) given the current meta-omics explorations on mechanisms of cyanobacterial succession, how to combine the extensive data to draw general conclusions is a challenge in the future; (4) the dominant niche of high temperature-adapted cyanobacteria genera will be further reinforced with global warming and elevated carbon dioxide in the future; (5) considering the causes and future developments of cyanobacterial blooms, the management strategies for controlling cyanobacterial blooms include reducing external nutrient input and removing internal nutrient in sediment, artificial mixing waters to decrease buoyancy of cyanobacteria, and biological control using allelopathy of aquatic plants and/or enhancing zooplankton feeding.

Mixotrophic Ochromonas Addition Improves the Harmful Microcystis-Dominated Phytoplankton Community in In Situ Microcosms.

Driven by global warming and eutrophication, outbreaks of cyanobacterial blooms have severely impacted ecosystem stability and water safety. Of the organisms used to control cyanobacteria, protozoa can highly resist cyanotoxins, efficiently control cyanobacterial populations, and show considerably different feeding strategies from those of metazoans. Thus, protozoa have great potential to control harmful cyanobacteria and improve phytoplankton composition in eutrophic waters. To evaluate the actual effects of protozoa in controlling cyanobacteria and improving the phytoplankton community structure in the field, an in situ microcosm study was performed using a flagellate Ochromonas gloeopara that ingests Microcystis. Results showed that adding Ochromonas reduced the cyanobacterial populations and increased the chlorophyte and diatom proportions. Furthermore, the species richness and diversity of the phytoplankton community were enhanced in microcosms with Ochromonas. Additionally, there was a gradual increase in the chlorophyte population in the unicellular Microcystis control, while Ochromonas addition significantly accelerated the replacement of dominant species. This study was the first to show the practical effects of protozoa on controlling cyanobacteria in the field, highlighting that a reduction in in situ cyanobacteria via protozoa can improve the phytoplankton community structure, dredge the toxic cyanobacteria-dominated microbial food web, and mitigate harmful cyanobacteria risks in fresh waters.

Surfactants at environmentally relevant concentrations interfere the inducible defense of Scenedesmus obliquus and the implications for ecological risk assessment.

The ecotoxicology of surfactants is attracting wide attention due to the rapidly expanding global application. As interspecific relationships play one of the central roles in structuring biological communities, it is necessary to take it into risk assessments on surfactants. With this aim, our study investigated the interference of three common surfactants on the inducible defense of a freshwater phytoplankton Scenedesmus obliquus. Nonlethal environmentally relevant concentrations (10 and 100 µg L-1) of several surfactants were set up. Results showed that growth and photosynthetic efficiency of Scenedesmus were inhibited during first 96 h, but recovered in the later stage. Surfactants interfered inducible defense of Scenedesmus against Daphnia grazing, and the interference was related to chemical characteristics of surfactants. The anionic surfactant sodium dodecyl sulfate (SDS) enhanced the colony formation even without grazing cues, whereas fewer defensive colonies were formed under the effects of cationic surfactant benzalkonium bromide (BZK) and nonionic surfactant polyoxyethylene (40) nonylphenol ether (NPE). These findings highlighted the sensitivity of grazer-induced morphological defense of Scenedesmus to surfactants even at nonlethal concentrations, which potentially affects the energy and information flow between trophic levels. This study appeals for more attention to take interspecific relationships into consideration in assessing the potential ecological risk of pollutants.

High concentration of Phaeocystis globosa reduces the sensitivity of rotifer Brachionus plicatilis to cadmium: Based on an exponential approach fitting the changes in some key life-history traits.

Most coastal waters are at risk of heavy metal pollution, and the biomass of primary producer phytoplankton always fluctuates, which usually causes zooplankton to be exposed in different concentrations of food and heavy metal. Phytoplankton abundance and heavy metal may interact on zooplankton. Therefore, to assess the definite interactive way, in this study we investigated the combined effects of different cadmium (Cd) levels and Phaeocystis globosa concentrations on some key life-history traits of the rotifer Brachionus plicatilis. Results showed that the Cd level and P. globosa concentration had a significant interaction on the key life-history parameters of the rotifer. Mid-level algal concentrations (5-36 × 104 cells mL-1) had an apparent effect on brood production and the number of rotifers producing offspring at high Cd level. The time to first reproduction exponentially decreased with increasing P. globosa concentrations under any Cd levels and then subsequently reached a constant value. With increasing P. globosa concentration, the total number of offspring exponentially increased and then reached the asymptotic value; the survival time under any Cd levels exponentially decreased with the increasing P. globosa concentration and subsequently tended to be a constant value. Without Cd, the low P. globosa concentration only decreased the reproduction of rotifers. However, the extreme low P. globosa concentration (1-3 × 104 cells mL-1) under higher Cd level (0.0354 mM) completely inhibited the reproduction and also shorten the survival time. Higher Cd level decreased the asymptotic total offspring per rotifer and survival time. High concentration of P. globosa can reduce the sensitivity of rotifer to heavy metal. However, the negative effects could not be eliminated completely by the increasing P. globosa concentration. The findings indicated that ecotoxicological studies on the toxicity of heavy metal need to consider the effects of food concentrations, which contributes to understanding the diverse tolerance of zooplankton to heavy metals.

Magnesium depletion suppresses the anti-grazer colony formation in Scenedesmus obliquus.

In aquatic ecosystems, many phytoplankton species have evolved various inducible defense mechanisms against the predation. The expression of these defenses is affected by environmental conditions such as nutrient availability. Here, we investigated the anti-grazer colony formation in Scenedesmus obliquus at different magnesium concentrations (0-7.3 mg L-1 Mg2+) in the presence of zooplankton (Daphnia)-derived infochemicals. Results showed that at adequate Mg2+, S. obliquus formed high proportions of multi-celled (e.g., four- and eight-celled) colonies, resulting in significantly increased number of cells per colony in response to Daphnia filtrate. On the other hand, in Mg2+-deficient treatment, the proportion of multi-celled colonies decreased, together with reduced algal growth rate and photosynthetic efficiency. Finally, the treatment without Mg2+ strongly suppressed the formation of large colony (mainly eight-celled colonies), whereas the algal growth rate was comparable to that in Mg2+ sufficient treatment. Despite the inhibition of colony formation, the time reaching the maximum number of cells per colony was not affected by the Mg2+ concentration, which generally took three days in all groups. Our results indicate that Mg2+ deficient/absent environments significantly reduced anti-grazing colony formation but not the algal growth, suggesting strong dependability of this morphological defensive trait to magnesium fluctuation in S. obliquus.