Effective extraction of fluoroquinolones from water using facile modified plant fibers / 药物分析学报

In this study,ecofriendly and economic carboxy-terminated plant fibers(PFs)were used as adsorbents for the effective in-syringe solid phase extraction(IS-SPE)of fluoroquinolone(FQ)residues from water.Based on the thermal esterification and etherification reaction of cellulose hydroxy with citric acid(CA)and sodium chloroacetate in aqueous solutions,carboxy groups grafted onto cotton,cattail,and corncob fibers were fabricated.Compared with carboxy-terminated corncob and cotton,CA-modified cattail with more carboxy groups showed excellent adsorption capacity for FQs.The modified cattail fibers were reproducible and reusable with relative standard deviations of 3.2％-4.2％within 10 cycles of adsorption-desorption.A good extraction efficiency of 71.3％-80.9％was achieved after optimizing the extraction condition.Based on carboxylated cattail,IS-SPE coupled with ultra-performance liquid chromatography with a photodiode array detector was conducted to analyze FQs in environmental water samples.High sensitivity with limit of detections of 0.08-0.25 μg/L and good accuracy with recoveries of 83.8％—111.7％were obtained.Overall,the simple and environment-friendly modified waste PFs have potential appli-cations in the effective extraction and detection of FQs in natural waters.

Preparation of molecularly imprinted polymers and application in a biomimetic biotin-avidin-ELISA for the detection of bovine serum albumin.

Immunoassay as a primary analytical tool is widely applied in the field of analysis. However, biological antibodies used in the routine immunoassay exhibit high cost and poor stability. Herein, in this study, a biomimetic ELISA method using molecularly imprinted polymers as a alternative to antibodies was developed. The molecularly imprinted polymers were prepared through dopamine polymerization using Fe3O4 nanoparticles as magnetic nuclei, bovine serum albumin as a template, dopamine as a functional monomer and crosslinking agent. The molecularly imprinted polymers were characterized by scanning electron micrographs, transmission electron microscope, X-ray photoelectron spectroscopy, thermogravimetric analysis, vibrating sample magnetometer and X-ray diffractometer, respectively. The detection range of the established biomimetic ELISA method was 5-1000 µg mL-1. The method exhibited high selectivity for bovine serum albumin compared with other proteins and good recovery ranging from 89.0% to 102.3% was obtained from spiked bovine serum samples. The results showed that the method by using molecularly imprinted polymers as biomimetic antibodies could be used to detect bovine serum albumin rapidly in bovine serum with a high sensitivity and accuracy. This study demonstrates that the preparation of molecularly imprinted polymers by dopamine polymerization can produce material with high affinity and potential to replace antibodies in biomimetic ELISA for protein detection.