RESUMO
BACKGROUND: Serum lipid profiles may be influenced by thyroid function, but the detailed mechanism remains unclear. Increasing evidence suggests that thyrotropin (TSH) may exert extra-thyroidal effects. The goal of this study was to evaluate the relationship between serum TSH levels and the lipid profiles in euthyroid non-smokers with newly diagnosed asymptomatic coronary heart disease (CHD). METHODS: This was a retrospective study of 406 euthyroid non-smokers (187 males and 219 females) with newly diagnosed asymptomatic CHD from 2004 to 2010 in Jinan, China. Lipid parameters and the levels of TSH, FT3, and FT4 were determined. Multiple linear regression analysis and Logistic regression analysis were used to assess the influence of TSH on the lipid profiles and the risks of dyslipidemia. RESULTS: The TSH level, even within the normal range, was positively and linearly correlated with total cholesterol (TC), non-high density lipoprotein cholesterol (non-HDL-C) and triglycerides (TG) (Beta = 0.173, 0.181 and 0.103, respectively, P < 0.01 in all). With 1 mIU/L rise of TSH, the levels of TC, TG and non-HDL-C will increase by 1.010, 1.064, and 1.062 mmol/L, respectively. The odds ratio of hypercholesterolemia and hypertriglyceridemia with respect to the serum TSH level was 1.640 (95% CI 1.199-2.243, P = 0.002) and 1.349 (95% CI 1.054-1.726, P = 0.017), respectively. CONCLUSIONS: TSH levels were correlated in a positive linear manner with the TC, non-HDL-C and TG levels in euthyroid non-smokers with newly diagnosed asymptomatic CHD. TSH in the upper limits of the reference range might exert adverse effects on lipid profiles and thus representing as a risk factor for hypercholesterolemia and hypertriglyceridemia in the context of CHD.
Assuntos
Doença das Coronárias/sangue , Lipídeos/sangue , Glândula Tireoide/fisiopatologia , Tireotropina/sangue , Idoso , Idoso de 80 Anos ou mais , Doenças Assintomáticas , Glicemia/análise , Colesterol/sangue , Doença das Coronárias/diagnóstico , Doença das Coronárias/etiologia , Dislipidemias/sangue , Dislipidemias/complicações , Feminino , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Análise Multivariada , Valores de Referência , Estudos Retrospectivos , Fatores de Risco , Fumar , Tiroxina/sangue , Tri-Iodotironina/sangueRESUMO
To search autoantigens in autoimmune pancreatitis (AIP), we have screened the human pancreas cDNA library with a patient's serum and obtained 10 positive clones. Seven out of 10 clones were amylase alpha-2A, the autoantibody to which was specifically detected in sera from patients with AIP and fulminant type 1 diabetes (FT1DM) [T. Endo, S. Takizawa, S. Tanaka, M. Takahashi, H. Fujii, T. Kamisawa, T. Kobayashi, Amylase alpha-2A autoantibodies: novel marker of autoimmune pancreatitis and fulminant type 1 diabetes mellitus, Diabetes 58 (2009) 732-737]. Sequencing of 1 out of remaining 3 positive clones revealed that it was identical to heat shock protein 10 (HSP 10) cDNA. Using a recombinant HSP 10, we have developed enzyme-linked immunosorbent assay (ELISA) system for detecting autoantibodies against HSP 10. We found that autoantibody against HSP 10 was also produced with high frequency in sera from patients with AIP (92%) and FT1DM (81%), but not in chronic alcoholic pancreatitis (8%) or healthy volunteers (1.4%). These results suggest that an autoantibody against HSP 10 is also a new diagnostic marker for both AIP and FT1DM.
Assuntos
Autoantígenos/imunologia , Doenças Autoimunes/imunologia , Chaperonina 10/imunologia , Diabetes Mellitus Tipo 1/imunologia , Pancreatite/imunologia , Adolescente , Adulto , Idoso , Autoantígenos/análise , Autoantígenos/genética , Doenças Autoimunes/sangue , Chaperonina 10/análise , Chaperonina 10/genética , Diabetes Mellitus Tipo 1/sangue , Ensaio de Imunoadsorção Enzimática , Feminino , Biblioteca Gênica , Humanos , Masculino , Pessoa de Meia-Idade , Pancreatite/sangue , Análise de Sequência de DNA , Adulto JovemRESUMO
Objective To investigate HLA-DPB1, DQB1 gene alleles and their correlation with autoantibodies in type 1 diabetes mellitus. Methods Sequence-based typing was used to determine the alleles of HLA-DPB1 and -DQB1 in 68 patients with type 1 diabetes and 50 healthy controls. Autoantibodies 〔glutamic acid decarboxylase antibody (GADA), islet cell antibody (ICA), insulin autoantibody (IAA)〕were detected by ELISA. Results Compared with the controls, the frequencies of DPB1*0402 and DQB1*0301 were significantly lower in type 1 diabetics (11.76%, 5.15% vs 30.00%, 22,00% respectively, P
RESUMO
Circulating nitric oxide(NO)and endothelin (ET) levels were determined in 30 normal subjects and 63 diabetic patients.The results showed that:serum NO level was significantly higher in DM I group(68.66?12.37?mol/L)and DM Ⅱ group(63.43?11.09?mol/L)than in normal subjects(44. 92?9.04?mol/L,P0.05).Plasma ET level was markedly elevated in DM Ⅰ group(68.92?11.96ng/L,P
