Factors affecting the mortality of weaned piglets during commercial transport between farms.

Factors affecting the number of weaned piglets found dead after commercial transport between farms were studied by means of multilevel Poisson regression. Information relating to 58,682 piglets during 109 journeys in different EU countries was collected at the end of each journey by means of questionnaires. Overall, 0.07 per cent of all the transported piglets were found dead on arrival, and deaths of piglets were recorded in 13.8 per cent of journeys. The final regression model, which was calculated using the complete records from 105 journeys, included the total duration of the journey, the mean outside temperature during the journey, whether the piglets were fasted before transport, whether drinking water was provided, the type of ventilation in the vehicle and the interaction between journey duration and the mean outside temperature. The duration of the journey and the mean outside temperature showed a significant interaction effect, with a gradual increase in the predicted number of dead piglets with increasing journey duration as the outside temperature increased. Providing the piglets with drinking water and having mechanically assisted ventilation (fans) in the vehicle during the journey significantly reduced the number of deaths, as did fasting the piglets before transport.

Noise levels in lairages for cattle, sheep and pigs in abattoirs in England and Wales.

Levels of sound intensity were measured over periods of 24 hours in 34 abattoir lairages in England and Wales. The mean integrated range in 12 cattle lairages was 52 to 79 dB(A), in 11 sheep lairages, 45 to 76 dB(A) and in 11 pig lairages, 46 to 87 dB(A). In general, the pig lairages were the noisiest, with spot peak recordings of up to 110 dB(A). Typically, the sound intensities in all the lairages were 10 to 20 dB(A) higher during the working day than at night. In many sheep lairages, high intensities of sound were recorded frequently throughout the night, but others were very quiet, below 40 dB(A). Vocalisations were the major sources of noise in the pig and cattle lairages, but there were variations between them. There were high intensities of sound from handling systems (80 to 90 dB[A]), and ventilating fans (70 to 80 dB[A]).

Prevalence and factors associated with it, of birds dead on arrival at the slaughterhouse and other rejection conditions in broiler chickens.

1. Standardised data on husbandry were recorded for a flock of birds in one house on each of 150 broiler farms in the UK during the 4 d prior to slaughter. 2. For each flock, the incidence of birds found dead on arrival (DoAs) and the Meat Hygiene Service carcase rejection records were recorded at the slaughterhouse. 3. The mean percentage of birds in each flock found DoA was 0.12% (range 0-0.64%) and the mean percentage of Total Carcase Rejects (TCRs) for each flock was 1.23% (range 0.07-5.51%). 4. A general linear model was developed to examine factors associated with flock percentage DoAs. Assuming a linear relationship, all other factors remaining the same, a one percentage point (PP) increase in small/emaciated birds will result in a 0.155 PP increase in DoAs and a 1 PP increase in wheat in diet 4 will result in a 0.003 PP decrease. An increase by one in the total number of vaccines administered will cause a 0.029 PP decrease in DoAs, a 1 g increase in live weight at slaughter will be associated with a 0.000043 PP increase and a 1 PP increase in mortality on farm would be associated with a 0.000044 PP increase. A 1 PP increase in Ross birds decreases DoAs by 0.0004 PPS: there is also a seasonal effect. 5. The model developed for flock percentage TCRs found that a 1 PP increase in wheat in diet 3 will result in a 0.052 PP decrease in TCRs and a 1 PP increase in Ross birds will cause a 0.009 PP decrease. A 1 PP increase in birds culled on farm will be associated with a 0.03 PP increase in TCRs and the diagnosis of disease during the flock cycle increases TCRs by 0.397 PPs. A one day increase in age at slaughter will result in a 0.046 PP increase in TCRs.

Factors affecting the mortality of pigs being transported to slaughter.

A multilevel logistic regression model was used to identify and quantify the effects of factors affecting the mortality of pigs being transported to slaughter under commercial conditions. A survey of 739 journeys to 37 slaughterhouses in five eu countries was carried out, and information potentially related to the welfare and mortality of the pigs and the number of injuries were recorded. The average temperature during the journey, its duration, the average loading time per pig, the recorded injuries, fasting before transport, and the interaction between fasting and journey duration were used in the final model. The average mortality was 0.11 per cent and the average proportion of injured pigs was 0.36 per cent, and these figures were significantly correlated (P<0.001). In 29 per cent of the journeys the pigs were not fasted before being loaded, which doubled the risk of mortality irrespective of whether the pigs were injured or not. The risk of mortality increased with average temperature. In journeys with fasted pigs that did not have any recorded injury, average temperature was more important than the duration of the journey. The risk of mortality increased as the average time taken to load them decreased, and the risk was highest when the pigs were not fasted and when injuries were recorded. Other factors such as the country, loading density, availability of drinking water and type of ventilation did not affect the risk of mortality.

Effects of well-boat transportation on the muscle pH and onset of rigor mortis in Atlantic salmon.

During the transport of salmon (Salmo salar), in a well-boat, 10 fish were sampled at each of six stages: in cages after crowding at the farm (stage 1), in the well-boat after loading (stage 2), in the well-boat after eight hours transport and before unloading (stage 3), in the resting cages immediately after finishing unloading (stage 4), after 24 hours resting in cages, (stage 5) and in the processing plant after pumping from the resting cages (stage 6). The water in the well-boat was at ambient temperature with recirculation to the sea. At each stage the fish were stunned percussively and bled by gill cutting. Immediately after death, and then every three hours for 18 hours, the muscle pH and rigor index of the fish were measured. At successive stages the initial muscle pH of the fish decreased, except for a slight gain in stage 5, after they had been rested for 24 hours. The lowest initial muscle pH was observed at stage 6. The fishes' rigor index showed that rigor developed more quickly at each successive stage, except for a slight decrease in rate at stage 5, attributable to the recovery of muscle reserves.

Aspects of meat and eating quality of broiler chickens reared under standard, maize-fed, free-range or organic systems.

1. The effects of rearing chickens using standard, maize-fed, free-range or organic production systems on meat quality and sensory characteristics were evaluated. The standard system used either Ross or Cobb birds with a slaughter age of approximately 40 d, the other systems in the trial used Hubbard birds with considerably older slaughter ages, up to 72 d in the case of organic systems. 2. Paired breast fillets from 120 birds, 30 from each rearing system, were used. The meat quality variables, ultimate pH, colour coordinates L*, a*, b* and water-holding capacity were measured and taste panel assessments were made using 8-point category scales of texture, juiciness, abnormal flavour, flavour liking and overall flavour. 3. There were significant differences between rearing systems, with fillet muscles from birds grown under the standard system having a higher ultimate pH. Differences were also seen in colour with fillets from birds reared under a standard system having a smaller hue angle than those grown using the maize-fed system which had the highest. 4. Fillets from birds reared in the standard system were rated by the taste panel as more tender and juicy. There were no significant differences in chicken flavour. Based on hedonic assessments of flavour liking and overall liking, by a small panel of assessors, meat from birds produced in the standard system was most preferred and that from organic systems the least preferred. Meat from free-range and maize-fed systems was intermediate in preference. This result reveals a trend, but does not infer consumer acceptance.

Multi-element survey of allotment produce and soil in the UK.

In 2004, a survey of 12 metals was carried out in six rural and six urban allotment sites across the UK. A total of 215 samples of vegetables and 36 samples of soft fruit were analysed using ICP-MS, and 51 samples of soil using ICP-OES. On a fresh weight basis, Cu levels in produce ranged 0.119-2.271 mg kg(-1). The highest levels measured (in mg kg(-1)) were Pb 0.164 in a sample of blackcurrants, Cd 0.039 in spinach, Hg 0.003 in curly kale and As 0.025 in raspberries. The lowest concentrations for these metals were below the level of detection (LOD) in numerous samples. The majority of Pt samples were below LOD and all were under the limit of quantification (LOQ). The results of the survey showed that the concentrations of the metals in vegetables, fruit and soils were consistent with previous studies and, generally, at low levels.

Factors affecting the prevalence of foot pad dermatitis, hock burn and breast burn in broiler chicken.

1. Standardised data on flock husbandry were recorded on 149 broiler farms during the 4 d prior to slaughter. 2. Birds were examined at the slaughterhouse for contact dermatitis lesions. Foot pad dermatitis score (FPDS) and hock burn score (HBS) were measured on five point scales. Carcase rejection data were also collected. 3. The mean percentage of birds in each flock with: moderate or severe foot lesions was 11.1% (range 0-71.5%); moderate or severe hock burn was 1.3% (range 0-33.3%); and, breast burn was 0.02%. 4. A general linear model was developed to examine factors associated with mean flock FPDS. Assuming a linear relationship, within the range of data collected and with all other factors remaining the same, every 1% increase in the proportion of Genotype A birds in the flock was associated with an increase in mean FPDS of 0.003, every one-point increase in litter score was associated with a 0.326 increase in mean FPDS and every one-point increase in flock mean HBS was associated with a 0.411 increase in mean FPDS. Flock mean FPDS was associated with feed supplier and was higher in winter. 5. The general linear model developed for flock mean HBS, found that every one-point increase in mean FPDS increased mean HBS by 0.090, every one-point increase in litter score increased HBS by 0.119 and, every 1% increase in small/emaciated birds decreased mean HBS by 0.333. Reduced HBS was also associated with increased final litter depth, younger slaughter age and an increased percentage of dietary wheat. For every 1% increase in Genotype A birds, a decrease in flock mean HBS of 0.003 would be expected. 6. An effect of hatchery was also identified.

Levels of foot pad dermatitis in broiler chickens reared in 5 different systems.

1. The prevalence and severity of foot pad dermatitis (FPD) was examined in 359 flocks of broiler chickens comprising about 3.93 million birds produced on 91 farms and reared under 5 types of system: standard intensive, free-range, organic, systems complying with the RSPCA Freedom Food scheme, and corn-fed. 2. The mean prevalence of FPD in standard systems was 14.8%. Flocks that had access to the outside (free-range and organic systems) showed higher prevalence of FPD than those kept entirely indoors (standard, corn-fed and Freedom Food systems). The lowest prevalence of FPD (9.6%) and severity of the lesions occurred in Freedom Food systems, and the highest prevalence (98.1%) and severity in organic systems. 3. The implication from the results is that there are large variations in the prevalence and severity of FPD in birds reared under different systems and this needs to be taken into account when assessing the overall welfare associated with each type of system.

Comparison of the effects of fan-assisted and natural ventilation of vehicles on the welfare of pigs being transported to slaughter.

A total of 417 pigs with an estimated average liveweight of 91 kg were transported to slaughter in three journeys, carried out in late June, each consisting of a three-hour drive followed by a one-hour holding period at the slaughter plant before they were unloaded. The pigs were accommodated on the lower two decks of a three-deck articulated lorry, each deck having five equal-sized pens; half were kept in naturally ventilated pens and half in pens with fan-assisted ventilation. The pigs' body temperature was recorded as the temperature of the blood lost at exsanguination, and as the temperature of the inner surface of the ear measured by thermal imaging. The pigs carried on the upper deck were hotter than those carried on the lower deck, and the pigs carried at the front of the vehicle were hotter than those at the back. There were small differences between the temperatures of the pigs in the different pens, but overall the temperatures of the pigs kept in pens with fan-assisted ventilation were no lower than those of the pigs kept in pens with natural ventilation. The higher temperature of the pigs transported in the front of the vehicle was associated with significantly higher serum concentrations of cortisol and activities of creatine kinase, and a tendency to higher albumin concentrations and osmolality, suggesting that they may have been exposed to more physical and psychological stress, and were possibly slightly more dehydrated, than the pigs in the rearmost pens. The type of ventilation had no effect on the blood composition of the pigs.

Measurements of footpad dermatitis in broiler chickens at processing plants.

A four-point photographic scale was developed to score the severity of lesions of footpad dermatitis (FPD) in broiler chickens. There was a linear relationship between the square root of the relative area of the lesions and their scores, and good agreement between different assessors using the scale. The scale was used to assess samples of 100 birds from each of 190 flocks slaughtered at two uk processing plants in 2002 and 2003; 12 of the flocks (6.3 per cent) had no lesions, but the others had lesions of different prevalences and severity. The maximum proportion of affected birds in a flock was 92 per cent. In the 178 affected flocks, 84.0 per cent of the birds had no lesions and 16.0 per cent had some evidence of FPD. The overall unweighted prevalence of birds with FPD in all 190 flocks sampled was 18.1 per cent; 10.2 per cent had only small lesions, on average equivalent in area to 2.1 per cent of the total area of the foot, 6.2 per cent had lesions on average equivalent to 6.6 per cent of the area of the foot, and 1.7 per cent had lesions on average equivalent to 21.5 per cent of the area of the foot. There were differences between the two plants in the overall prevalence and severity of FPD, but this may have been due to the fact that the plants were sampled at different times of the year.

Effects of stocking density, flock size and management on the welfare of laying hens in single-tier aviaries.

Management practices, stocking rate and flock size may affect laying hen welfare but there have been few replicated studies in commercial non-cage systems that investigate this. This study used a broad range of physical and physiological indicators to assess the welfare of hens in 36 commercial flocks. Six laying period treatments were examined with each treatment replicated 6 times. It was not possible to randomly allocate treatments to houses, so treatment and house were largely confounded. Three stocking rates were compared: 7 birds/m(2) (n = 2450), 9 birds/m(2) (n = 3150) and 12 birds/m(2) in either small (n = 2450) or large (n = 4200) flocks. In addition, at 12 birds/m(2), in both small and large flocks, birds were subjected to either standard (SM) or modified (MM) management. MM flocks had nipple drinkers and no nest-box lights. Bone strength, fracture incidence, heterophil:lymphocyte (H:L) ratio, live weight, organ weights, serum creatine, serum osmolality, muscle pH and faecal corticosterone were measured on samples of birds at the end of the rearing period and at the end of lay. During the laying period, mortality, production and integument condition were recorded at regular intervals. Birds housed at 9 birds/m(2) had higher mortality than birds housed at 12 birds/m(2) by the end of lay, but not higher than birds housed at 7 birds/m(2). Birds housed at 7 and 9 birds/m(2) had lower percent liver weight, and worse plumage condition than most of the 12 bird/m(2) treatments. Modified management tended to improve plumage condition. There were no clear effects of flock size on the welfare indicators recorded. At the end of the rearing period fracture incidence was almost negligible and H:L ratio was within a normal range. By the end of lay fracture incidence was 60% and H:L ratio was high, with no treatment effect for either measure. This, together with information on faecal corticosterone, feather loss and mortality, suggests that the welfare of birds in all treatments was relatively poor by the end of lay.

Preliminary study to examine the utility of using foot burn or hock burn to assess aspects of housing conditions for broiler chicken.

1. Eleven broiler chicken farms, representing 4 production system types, were visited during the last 5 d of the flock cycle: bird and flock details were recorded. Litter friability was assessed at 9 sites within the house, atmospheric ammonia was measured at three sites and bird cleanliness was assessed on a numerical rating scale. 2. For these flocks, hock burn, foot burn and breast burn were measured at the processing plant by standardised assessors. 3. Significant correlations were identified between the percentage of birds with foot burn and average litter score, average house ammonia concentrations and feather score. 4. No correlation was found between the percentage of birds with hock burn or breast burn and average litter scores, average ammonia concentrations or feather score. 5. No correlation was found between stocking density and foot burn, hock burn or breast burn.6. If confirmed, these findings may have implications for the draft EU Broiler Directive, for which it is proposed that permitted stocking density on farm may be determined by the incidence and severity of contact dermatitis measured on plant.

Estimating the body temperature of groups of pigs by thermal imaging.

Measurements on 28 pens of pigs containing 384 animals to be slaughtered at a commercial abattoir showed that the mean ear temperatures of the pigs in each pen, measured with a thermal imaging camera, were significantly correlated (r=0.71, P<0.001) with the mean temperature of the blood the pigs lost at exsanguination. In measurements on 220 pigs in 16 of the 28 pens, the mean activity of serum creatine kinase was positively correlated with the mean ear temperature (r=0.55, P<0.05) and the mean concentration of serum cortisol was positively correlated with the mean blood temperature (r=0.50, P<0.05), suggesting that the hotter pigs were suffering from a higher level of stress.

Multi-element survey of wild edible fungi and blackberries in the UK.

A survey of 12 metals including lead (Pb), copper (Cu), cadmium (Cd), mercury (Hg), arsenic (As) and platinum (Pt) was carried out using ICP-MS in 34 samples of wild fungi and 48 samples of wild blackberries collected from sites across the UK. On a fresh weight basis (mg/kg) levels of Pb were in the range 0.003-5.990, Cu 0.596-34.800, Cd<0.001-19.6, Hg<0.001-4.150, As 0.001-0.972 and Pt (microg/kg) 0.006-0.200, with higher concentrations found in fungi than in blackberries. The results of the survey showed that the concentrations of the metals were consistent with previous studies, where available. Concentrations in wild fungi of Pt, tin (Sn), and titanium (Ti) were significantly higher at urban sites than at rural sites. Urban blackberries had significantly higher levels of Pb, Ti, and Cd than rural ones, but lower levels of manganese (Mn). Pb, Ti and Sn concentrations were significantly higher in blackberries sampled near main roads rather than in rural areas.

The colour of the adductor muscle as a predictor of pork quality in the loin.

The relation between measurements of colour made in the m. adductor (AD) at 45min or 20 h post mortem and the quality, assessed subjectively in terms of colour and waterholding capacity, of the m. longissimus (LD) in the loin was examined. The study used data from 100 pig carcasses exhibiting a wide range of meat quality from extreme PSE (pale, soft and exudative) to extreme DFD (dark, firm and dry). The subjective assessments were confirmed by objective measures of paleness (reflectance) and waterholding capacity (drip loss in storage) in the LD. Lightness (L(∗)) measured at 20h post mortem in the AD was the best potential predictor of loin muscle quality, explaining 59% of the variation in subjective and objective quality measures. Comparable measurements at 45min post mortem explained between 21% and 44% of the variation. The equation that described the relation between AD Lightness (L(∗)) and subjectively assessed LD quality was derived. This could be used to transpose the AD L(∗) values from a population of slaughtered pigs into nominal subjective scores for the LD, allowing the frequency of the five subjective quality groups (extremely DFD, slightly DFD, normal, slightly PSE, and extremely PSE) in the population of carcasses to be defined.

The response of pigs to being loaded or unloaded onto commercial animal transporters using three systems.

Groups of pigs were subjected to three different systems of loading and unloading to and from commercial animal transporters. The systems under trial were: the use of a hydraulic tail-lift, a tail board ramp at an angle of 18 degrees and a modular system. The module was a container 2 m x 2.4 m x 1 m high into which the pigs were loaded. The module could then be lifted on and off the lorry with a fork lift truck. Non-invasive monitoring techniques were used to study the responses of the pigs. A subjective handling score, time taken to load and unload, skin temperature, heart rate and salivary cortisol were recorded. Loading and unloading were subjectively assessed as being easiest and quickest using the modular system, which also appeared to be less physically demanding for the animals, as evidenced by lower heart rate and a reduced maximum heart rate. However, with the modular system, elevated cortisol was found during the loading, unloading and resting periods. This could be interpreted as indicating that the pigs were unable to settle in the module and suffered some small degree of stress, which might become more of a problem during prolonged transport. Of the other two systems, there appeared to be little difference between the hydraulic tail-lift and the ramp.

Relationship between maximum daily temperature and mortality of broiler chickens during transport and lairage.

1. Based on records of all (n = 59 171 843) broiler chickens slaughtered over three years at one processing plant, the overall mortality of birds in transit was 0.126%. 2. There was a pronounced seasonal effect with increased mortality in the summer months, particularly June, July and August. Mortality increased when the maximum daily temperature (measured in the shade) rose above about 17 degrees C at the start of the summer. 3. Between 17.0 and 19.9 degrees C, mortality was 30% higher than at lower temperatures. Between 20.0 and 22.9 degrees C it increased 2.6-fold, and at temperatures of above 23 degrees C 6.6-fold. There was no evidence of birds dying from hypothermia at very low ambient temperatures (down to -1 degrees C). 4. The implication from the results is that above a maximum daily temperature of 17 degrees C steps may need to be taken to ameliorate the damaging effects of transport on bird welfare.

Defaecation and weight of the gastrointestinal tract contents after feed and water withdrawal in broilers.

1. Broilers were held under constant light for up to 24 h with access to feed and water, access to water only, or access to neither feed nor water. 2. Birds given continuous access to feed ate evenly over the 24-h period. Feed deprivation reduced drinking behaviour to about half of that when feed was available. Deprivation of feed, or of feed and water, had similar effects on defaecation and the reduction in the weight of the gut contents. It reduced the overall number of defaecations over the 24-h period to about 40% of that in birds given food and water, and the weight of the contents by about 75%. The maximum effect of deprivation on defaecation was seen after about 4 h, and on gut contents weight after about 12 h. 3. With longer fasting the contents of most parts of the gut, except the caeca, became wetter. 4. The findings may have implications for the practice of preslaughter food withdrawal to reduce the potential for soiling of the live birds and contamination of the carcase after slaughter. However, the likelihood is that relatively short overall feed deprivation times of not more than 8 to 12 h before killing may be desirable, but specifying firm recommendations on optimal feed withdrawal times requires further work.