RESUMO
Cytomegalovirus (CMV) infection is one of the most common infectious complications following hematopoietic stem cell transplantation (HSCT); however, cases involving multiple organs at the same time are rare. The present study describes a case of CMV pneumonia combined with CMV DNAemia and CMV cystitis after HSCT. A 33-year-old male patient with acute myeloid leukemia was treated with HSCT. The first month after HSCT, the patient developed a cough and shortness of breath. At 2 months post-HSCT, the patient developed hematuria. The CMV DNA levels in the blood and urine were elevated; bronchoalveolar lavage fluid (BALF) was also positive for CMV DNA. Heterotypic cells exhibiting a large nuclear morphology were observed in the BALF and bronchial brushes. Recurrent and progressive ground-glass opacities were evident on chest computed tomography. The patient was diagnosed with CMV pneumonia complicated by CMV DNAemia and CMV cystitis, and was treated with a combination of ganciclovir and foscarnet, along with immunoglobulin therapy. The patient was cured and discharged. It was determined that the CMV DNA in the blood was inconsistent with that in the BALF, which delayed the early diagnosis of CMV pneumonia. The association between T-cell immune function and the therapeutic efficacy for CMV multi-organ infection following HSCT is known to be significant. Moreover, the timely administration of ganciclovir and foscarnet in combination with immunoglobulin therapy demonstrated favorable clinical outcomes.
RESUMO
A novel procedure was developed for the determination of arsenite (As(III)), arsenate (As(V)), monomethylarsonic (MMA) and dimethylarsinic acid (DMA) with ion chromatography-hydride generation-atomic fluorescence spectrometry (IC-HG-AFS) by employing a new gas-liquid separator (GLS). The effective separation of the four arsenic species was achieved in about 12min. With a sample loading volume of 20mul, the measurable minimum for As(III), DMA, MMA and As(V) were 0.02, 0.045, 0.043 and 0.166ng, respectively, along with relative standard deviations of 1.1, 1.1, 1.7 and 2.2% at the 100mugl(-1) level (n=6) for As(III), DMA, MMA and As(V), respectively. The present procedure was applied for the speciation of arsenic in underground water and in urine samples, and the sum of the four arsenic species by IC-HG-AFS was in good agreement with the total value by HG-AFS.
