RESUMO
OBJECTIVE: The aim of this study was to evaluate the percentage of pregnant women with negative Group B Streptococcus (GBS) screening results by culture at 35 weeks' gestation, who subsequently had positive GBS test results after 39 weeks' gestation. MATERIALS AND METHODS: From 2006 to 2007, we recruited 150 pregnant women who received routine GBS culture screening at 35 weeks' gestation with negative results, and who had repeat cultures and real-time polymerase chain reaction (RT-PCR) tests for GBS after 39 weeks' gestation. RESULTS: Two percent of pregnant women with GBS-negative results by culture screening at 35 weeks' gestation were GBS-positive at 39 weeks' gestation. CONCLUSION: It is necessary to perform a GBS test 4 weeks after an initial negative GBS culture at 35-37 weeks of gestation. RT-PCR provides a simple and rapid alternative method for detecting rectovaginal GBS colonization at the time of labor.
Assuntos
Técnicas Microbiológicas/métodos , Complicações Infecciosas na Gravidez/diagnóstico , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Infecções Estreptocócicas/diagnóstico , Streptococcus agalactiae/genética , Adolescente , Adulto , DNA Bacteriano/genética , Feminino , Humanos , Programas de Rastreamento/métodos , Projetos Piloto , Valor Preditivo dos Testes , Gravidez , Cuidado Pré-Natal/métodos , Estudos Prospectivos , Sensibilidade e Especificidade , Streptococcus agalactiae/crescimento & desenvolvimento , Streptococcus agalactiae/isolamento & purificação , Adulto JovemRESUMO
PURPOSE: To evaluate whether achieving a singleton pregnancy by IUI affects the results of first-trimester screening for Down syndrome compared to naturally conceived pregnancy. METHODS: Forty-nine IUI and 3059 naturally conceived singleton pregnancies were included in the study. Ovulation in IUI pregnancies was induced by clomiphene and human menopausal gonadotropin. Progesterone was given after insemination for 2 weeks. Down syndrome screening was performed using a combination of maternal age, fetal nuchal translucency, and maternal serum concentrations of free beta-hCG and PAPP-A during the period of 10-14 weeks' gestation. RESULTS: In IUI pregnancies, nuchal translucency thickness and the levels of PAPP-A were significantly higher. The values of free beta-hCG were not statistically different between the two groups. The screen-positive rate in IUI pregnancies was significantly higher (14.3% vs. 7.1%). CONCLUSIONS: Singleton pregnancies achieved by IUI have a higher screen-positive rate. Not only elder maternal age but also exogenous hormones given during the process of ovulation induction and after conception may play an important factor influencing positive screening results.
