[Evolution mechanism of ecosystem service value at the township-scale in Anxi County of Fujian Province, China]. / 福建安溪县乡镇土地生态系统服务价值的演变机制.

To clarify the interaction between land-use change and ecosystem service, the traditional ecosystem service valuation model was modified using the adjustment coefficients of biomass factor and socio-economic factor to evaluate ecosystem service value (ESV) of 24 towns in Anxi County from 1999 to 2019, aiming to understand the ecological response to land-use change. The results showed that ESV of 24 towns in Anxi County decreased gradually during the study period, with a decrease of 0.004 to 0.295 million yuan. Forestland and cultivated land made the largest contribution to ESV, followed by grassland and water bodies, whereas the contribution of other land types were negative. ESV could be increased by converting other land types into forestland, but be decreased by converting other land types into orchard land area (mainly tea plantation). The chemical fertilizer-monoculture management pattern of ratooning tea plantation reduced ESV, while the ecological cultivation of tea plantation could effectively improve soil microbial diversity and soil fertility, and finally enhance ESV.

[Bioinformation analysis of chorismate synthase in Baphicacantus cusia and other 78 species of plants].

Chorismate synthase(CS, EC:4.2.3.5) catalyses 5-enolpyruvy-shikimate-3-phosphate to form chorismate, which is the essential enzyme for chorismate biosynthesis in organisms. The amino acid sequences of CS from 79 species of higher plants were reported in GenBank at present. 125 amino acid sequences of CS from Baphicacanthus cusia and other 78 species of plants were predicted and analyzed by using various bioinformatics software, including the composition of amino acid sequences, signal peptide, leader peptide, hydrophobic/hydrophilic, transmembrane structure, coiled-coil domain, protein secondary structure, tertiary structure and functional domains. The phylogenetic tree of CS protein family was constructed and divided into eight groups by phylogenetic analysis. The homology comparison indicated that B. cusia shared a high homology with several plants such as Sesamum indicum, Nicotiana tabacum, Solanum tuberosum and so on. The open reading frame(ORF) of all samples is about 1 300 bp, the molecular weight is about 50 kDa, the isoelectric point(pI) is 5.0-8.0 which illustrated that CS protein is slightly basic. The ORF of CS we cloned in B. cusia is 1 326 bp, the amino acid residues are 442, the molecular weight is 47 kDa and pI is 8.11. The CS in B.cusia showed obvious hydrophobicity area and hydrophilicity area, no signal peptide, and may exists transmembrane structure areas. The main secondary structures of CS protein are random coil and Alpha helix, also contain three main structural domains which are an active structural domain, a PLN02754 conserved domain and a FMN binding site. The acquired information in this study would provide certain scientific basis for further study on structure-activity relationship and structure modification of CS in plants in the future.

[Effects of exogenous salicylic acid on protein expression level in Baphicacanthus cusia (Nees) Bremek leaves].

By using two-dimensional eletrophoresis method, this paper studied the protein expression level in Baphicacanthus cusia (Nees) Bremek leaves after sprayed with exogenous salicylic acid (SA). A total of significantly different 20 protein spots were obtained, among which, eight protein spots were indentified, being of ATP synthase, alpha tubulin, cell division protein, glyceraldehydephosphate dehydrogenase, and ACC oxidase, respectively. The expression abundance of all identified proteins was up-regulated, except for ACC oxidase which was down-regulated. Therefore, exogenous SA could affect the protein expression level in B. cusia leaves, and improve the plant resistance to environment stress and self-restoration capability.

[Proteomics of rice leaf and grain at late growth stage under different nitrogen fertilization levels].

Taking super-rice Liangyoupeijiu as test material, and by the method of two-dimensional gel electrophoresis (2-DE), this paper studied the changes in the leaf and grain proteomics of the variety at its late growth stage under different levels of nitrogen fertilization (1/2 times of normal nitrogen level, 20 mg x L(-1); normal nitrogen level, 40 mg x L(-1); 2 times of normal nitrogen level, 80 mg x L(-1)), with the biological functions of 16 leaf proteins, 9 inferior grain proteins, and 4 superior grain proteins identified and analyzed. Nitrogen fertilization could affect and regulate the plant photosynthesis via affecting the activation of photosynthesis-related enzymes and of CO2, the light system unit, and the constitution of electron transfer chain at the late growth stage of the variety. It could also promote the expression of the enzymes related to the energy synthesis and growth in inferior grains. High nitrogen fertilization level was not beneficial to the synthesis of starch in superior grain, but sufficient nitrogen supply was still important for the substance accumulation and metabolism. Therefore, rational nitrogen fertilization could increase the photosynthesis rate of flag leaves, enhance the source function, delay the functional early ageing, and promote the grain-filling at late growth stage.

[Studies on identification of Gryllotalpa by near-infrared diffuse reflectance spectrometry].

OBJECTIVE: To identify and analyse the different species, same species in different regions and confusion species. METHOD: Near-infrared diffuse reflectance spectrometry was used. RESULT: Clustering analysis showed that clustering relations were far among different Gryllotalpa species and close among the same species from different regions, and there were close relations among the same species from near regions and between Teleogryllus emmus and G. orientalis. CONCLUSION: Near-infrared diffuse reflectance spectrometry method can be used in classification and identification of Gryllotalpa.