Retraction Note: Study on the functions and mechanism of immune functions of human telomerase reverse transcriptase regulating dendritic cells treating sepsis.

The article "Study on the functions and mechanism of immune functions of human telomerase reverse transcriptase regulating dendritic cells treating sepsis", by H.-M. Chen, L.-Q. Wang, H.-P. Wan, H.-Z. Wei, L.-C. Ke, C.-Y. Liu, Q.-Y. Tan, published in Eur Rev Med Pharmacol Sci 2016; 20 (21): 4500-4507-PMID: 27874963 has been retracted by the Editor in Chief for the following reasons. Some concerns were raised on PubPeer (https://pubpeer.com/publications/3604386A706802443E51758A893D6F) about Figures 3, 4, and 5 showing some overlaps and similar bands in Western blots figures. Furthermore, there is a lack of information regarding the ethics approval for the study involving rats. The journal contacted the authors to request the original raw data and information regarding the ethical approval of the manuscript but never received a reply. Therefore, due to major concerns detected, the Editor in Chief mistrusts the results presented and decided to withdraw the manuscript. The corresponding author has been informed about the retraction. This article has been retracted. The Publisher apologizes for any inconvenience this may cause. https://www.europeanreview.org/article/11476.

[The microbiology of chronic rhinosinusitis with nasal polyps in different phenotype of peripheral blood eosinophils].

Objective:To investigate the association between immune status and microbial community in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) in different phenotype of peripheral blood eosinophils (PBEOS).Method:Retrospective analysis of clinical data of 125 patients with CRSwNP and and 49 control subjects was performed. According to the proportion of peripheral blood eosinophils, the patients with CRSwNP were divided into PBEOS normal group and PBEOS elevated group. The samples of nasal secretions in middle meatus were collected, and then isolated and identified by microbial culture. Differences of microbial colonization rate between the groups were analyzed. SPSS 19.0 software was used to analyze the data.Result:There were 61 cases in PBEOS normal group (0 5%) and 49 control subjects.In patient with CRSwNP, the positive rate of microbial culture was 84.4%, and the main microorganisms were Gram positive Aerobic and facultative anaerobic bacteria. The most common bacteria were coagulase negative staphylococci, Staphylococcus epidermidis (S. epidermidis) and Corynebacterium. The average positive rate of Staphylococcus aureus (S.aureus) was 10.4%, and S. epidermidis was 20.0%. The positive rate of S. aureus in the elevated group was higher than that in the normal group (17.2%, 3.3%), and the rate of S. epidermidis was lower (10.9%, 29.5%). The positive rate of S. epidermidis in the elevated group was also lower than that in control subjects (10.9%, 30.6%).Conclusion:In different PBEOS phenotypes, the colonization rate of S. aureus and S. epidermidis showed the opposite trend. In contrast to the normal goup, the colonization rate of S. aureus in the elevated group increased, and the rate of S. epidermidis decreased. At the same time, the colonization rate of S.epidermidis decreased in elevate group in contrast to the control subjests. This suggests that S. aureus may promote eosinophilic inflammatory response and S. epidermidis may promote noneosinophilic inflammatory response, which plays protective role.

[Functional rhinoplasty under nasal endoscope].

Objective:At different times and cultural background,people have different definition of beauty.With the improvement of economic level and culture quality,people are becoming more and more recognition of nasal appearance and function are equally important.Even the importance of the function of the nasal is higher than the importance of shape of the nose. Concurrent rhinoplasty (nasal dorsum and lateral nasal wall reduction) and functional sinus surgery (nasal septum reconstructory, middle turbinate,inferior turbinate surgery even extended to the nasal cavity expansion tecunhnique, sinusitis surgery) with endoscopic surgical surgical methods were discussed.Method:The study included 20 patients with deformed functional endoscopic rhinoplasty, 2010-2015 in Beijing Tongren Hospital,of ages 16-53, male 14 cases,female 6 cases, 11cases had history of trauma, all of 20 patients have unilateral nasal plugging. Operating methods, 17 patients were performed nasal endoscopic septoplasty and rhinoplasty at the same time. One case cocurrent endoscopic rhinodacryocystostomy.Two cases were performed Nasal endoscopic sinus operation. Only 5 cases use silica gel prosthesis, 15 cases use autologous cartilage in functional rhinoplasty.Result:Twenty patients compared with performed rhinoplasty,nasal septum reconstructory,middle turbinate,inferior turbinate surgery,nasal cavity expansion,sinusitis surgery separately,surgery is better,no other complicationgs occurred.Actually decredsed the number of surgical treatment and hospitalization expenses.Conclusion:Functional endoscopic rhinoplasty,over the same period it solved the nasal shape and nasal ventilation function.To avoid impact on ventilation function in the process of the rhinoplasty.Implements the unification of beauty and function.Reduce the surgical treatment of times and cost.No other complication compared to convertional surgery,high feasibility.

[Imaging charateristics of bony erosion and fungal culture analysis in allergic fungal rhinosinusitis].

Objective:To investigate the imaging characteristics of bony erosion, fungal culture and related factors in allergic fungal rhinosinusitis (AFRS). Method:Sixty cases of AFRS were reviewed in this study. The characteristics of Computed Tomography and Magnetic Resonance Imaging including positive rate of bony erosion, eroded sites, disease extension and sinus expansion were summarized. Fungal culture and identification of nasal secretion were done. The correlation between the degree of sinus expansion, species of fungi and bony erosion was evaluated by statistical analysis. Result:Of the 60 patients, 18 (30%) had bony erosion. There was a significant difference in the proportion of bone erosion among the sinuses (P<0.05). The most commonly eroded site was the ethmoid sinus. The orbit were the most common adjacent anatomic spaces to exhibit disease extension, and anterior cranial fossa is the second most common. Statistical analysis revealed a significant association(P<0.05)of bone erosion with sinus expansion. The positive rate of fungal culture was 51.3%, among which the most common is Aspergillus. Statistical analysis shows no association(P>0.05)of bone erosion with specific fungi. Conclusion:Bone erosion is an important imaging feature of AFRS. Bone erosion may cause by sinus expansion. Extension of disease into the orbit or intracranial cavity results from a natural progression of disease after erosion occurs. Specific fungal species that can cause bone erosion was found.

Study on the functions and mechanism of immune functions of human telomerase reverse transcriptase regulating dendritic cells treating sepsis.

OBJECTIVE: We analyzed the functions and mechanisms of immune functions of human telomerase reverse transcriptase regulating dendritic cells (DC) treating sepsis of mice models. MATERIALS AND METHODS: Eighty clean grade Balb/c animals aged from 6 to 8 weeks, weighted from 18 g to 22 g were selected for this study. The DC cells were harvested from the animals and cultivated to transfect with the recombinant eukaryotic expression plasmid hTERT-IRES2-EGFP construct. The LPS (E. coli 0111:B4, 5 mg/kg) was injected into the abdominal cavity of mice to establish sepsis models. Afterwards, animals were divided randomly into the sepsis group (A group), the group of hTERT transfecting DC (B group), the group of DC un-transfected (C group) with 25 mice in each group. 5 mice were in the normal control group (D group), without any treatment. An equivalent volume of normal saline was injected into the abdominal cavity of A group. Subsequently, 1 ml of cell suspension (105/ml) was transfected into B and C groups respectively. Five animals from A, B, C groups and one animal from group D were sacrificed after 24h, 48h, 72d, 7d and 10d respectively. RESULTS: It was found that median survival time of the group of hTERT transfecting DC was remarkably higher than that of the untransfected group and the sepsis group. The average scores of the pathology of kidney and intestine at each time were significantly lower than that of the other two groups (p<0.05). At each time point, in the group of hTERT transfecting DC, levels of CRP and Cr were remarkably lower than that of the other two groups; HLA-DR, CD40 of immune phenotype and the expression level of peripheral blood T cells MHC-II molecules were significantly higher than that of the other two groups; the expression level of IL-12 and TNF-a were significantly lower than that of the other two groups; apoptosis rate of DC were significantly lower than that of the other two groups; the content and activity of NF-κB were significantly higher than that of the other two groups (p<0.05). CONCLUSIONS: The telomerase reverse transcriptase gene can raise the expression and maturity of DC, reduce apoptosis, induce cytokine secretion, reduce the inflammatory response and increase the survival time.

Effect of microstructure of graphite on the nonreductive thermal ion emission in thermal ionization mass spectrometry.

The emission behavior of polyatomic ions in the ionization source of thermal ionization mass spectrometry (TIMS) was investigated. The results suggest that the presence of a graphite promoter plays a key role for the formation and stable emission of polyatomic ions, such as M(2)X(+), M(2)BO(2)(+), Cs(2)NO(2)(+), and Cs(2)CNO(+). Our data further implied that the intensity of M(2)X(+) and M(2)BO(2)(+) increases and the emission temperature decreases with increasing cationic and anionic radius. During the boron isotopic measurement using the Cs(2)BO(2)(+)-graphite-PTIMS method, the isobaric interference ion Cs(2)CNO(+) cannot be transformed from nitrate or organic compounds containing an amide group but can be induced by the existence of trace amounts of boron because of its special electron-deficiency property (B(3+)). Characterization on the planar crystalline structure of various graphite samples with SEM, TEM, and Raman spectroscopy confirmed the relationship of the emission capacity of polyatomic ions and the crystal microstructure of graphite and provides direct evidence that graphite with a perfect parallel and equidistant layer orientation shows a beneficial effect on the emission of polyatomic ions in TIMS. The mechanism study on the formation of polyatomic ions opens the possibility to establish high precision methods for isotopic composition analysis of more nonmetal elements with the TIMS technique.

Adsorption behavior of dinucleotides on bare and ru-modified glassy carbon electrode surfaces.

The interactive behavior of flavin adenine dinucleotide (FAD) with a bare glassy carbon electrode (GCE) and a Ru-modified GCE was investigated. The reduction of FAD at a GCE/ruthenium-modified GCE surface is a quasi-reversible, surface-controlled process, and our data implied that the attachment of FAD onto the surface is caused by nonspecific adsorption instead of covalent linkage, in which the adenine ring of FAD adopts a flat orientation on the GCE surface in neutral and dilute solutions in order to maximize the pi-pi stacking with the carbon surface and reorients to a perpendicular orientation as the surface gets more crowded. FAD desorption during the exchange with nicotinamide adenine dinucleotide (NAD+) is one order of magnitude slower than desorption in the absence of NAD+, which indicates a strong interaction between FAD and NAD+. General knowledge of the interactive behavior of NAD+ on a FAD-adsorbed GCE provides useful information for the design of a modified electrode surface for the generation of NADH from NAD+.

Emission of M2X+ cluster ions in thermal ionization mass spectrometry in the presence of graphite.

The emission of M2X+ cluster ions in thermal ionization mass spectrometry when graphite is loaded on the heating filaments was studied. The emission model of non-reductive thermal ionization of graphite was preliminarily discussed and factors influencing the thermal emission of M2X+ ions were investigated. The results show that the intensities of M2X+ cluster ions are related to ionic radius and crystal lattice energy, and possibly also to the solvation energies of ions. The intensities of M2Cl+ (M stands for K, Rb, and Cs) cluster ions, the M2Cl+/M+ ratios, and the 37Cl/35Cl ratios determined from M2Cl+ ion measurement usually increase with measurement time. The variation of the 37Cl/35Cl ratios determined from Cs2Cl+ ion measurement is lower than those based on K2Cl+ and Rb2Cl+ ion measurement, indicating the lowest isotopic fractionation.