RESUMO
Microbial remediation of oil-contaminated groundwater is often limited by the low temperature and lack of nutrients in the groundwater environment, resulting in low degradation efficiency and a short duration of effectiveness. In order to overcome this problem, an immobilized composite microbial material and two types of slow release agents (SRA) were creatively prepared. Three oil-degrading bacteria, Serratia marcescens X, Serratia sp. BZ-L I1 and Klebsiella pneumoniae M3, were isolated from oil-contaminated groundwater, enriched and compounded, after which the biodegradation rate of the Venezuelan crude oil and diesel in groundwater at 15 °C reached 63 % and 79 %, respectively. The composite microbial agent was immobilized on a mixed material of silver nitrate-modified zeolite and activated carbon with a mass ratio of 1:5, which achieved excellent oil adsorption and water permeability performance. The slow release processes of spherical and tablet SRAs (SSRA, TSRA) all fit well with the Korsmeyer-Peppas kinetic model, and the nitrogen release mechanism of SSRA N2 followed Fick's law of diffusion. The highest oil removal rates by the immobilized microbial material combined with SSRA N2 and oxygen SRA reached 94.9 % (sand column experiment) and 75.1 % (sand tank experiment) during the 45 days of remediation. Moreover, the addition of SRAs promoted the growth of oil-degrading bacteria based on microbial community analysis. This study demonstrates the effectiveness of using immobilized microbial material combined with SRAs to achieve a high efficiency and long-term microbial remediation of oil contaminated shallow groundwater.
