Bioaugmentation with an aerobic denitrifying bacterium with quorum quenching activity for improved nitrogen removal and reduced membrane fouling in anoxic/oxic membrane bioreactor.

In this study, an aerobic denitrifying bacterium with quorum quenching activity, Acinetobacter sp. WZL728, was inoculated into the anoxic/oxic membrane bioreactor (A/O-MBR) to study its effects on A/O-MBR performance. The pollutant removal and membrane fouling between A/O-MBR with WZL728 (EMBR) and A/O-MBR without WZL728 (CMBR) were compared. WZL728 increased the total nitrogen removal efficiency from 75.05% in CMBR to 91.03% in EMBR and extended the filtration cycle from 5.44 days in CMBR to 9.57 days in EMBR, which indicated that WZL728 improved the pollutant removal performance and mitigated membrane fouling of A/O-MBR. The concentration of N-acyl-homoserine lactones in the biocake of EMBR (EMBRB) was 11.23% of that in the biocake of CMBR (CMBRB). The content of extracellular polymeric substances (EPS) in EMBRB was 69.00% of that in CMBRB. The abundance of bacteria associated with EPS secretion (Alpthaproteobacteria) decreased and the abundance of bacteria associated with EPS degradation (Clostridia) increased in EMBRB. Valine, alanine and uridine diphosphate-N-acetylgalactosamine associated with protein and polysaccharide synthesis were significantly lower in EMBRB than those in CMBRB, which revealed the reason for the decrease of protein and polysaccharide content of EPS within EMBRB. This study provides useful information for improving A/O-MBR performance by probiotics.

Fast start-up of ANAMMOX biofilm processes at low temperatures by economical quorum sensing regulation: The importance of endogenous N-acyl-homoserine lactones from enhanced inoculated sludge.

The start-up of anaerobic ammonia oxidation (ANAMMOX) processes at low temperatures is quite difficult. In this study, the fast start-up (43 days) of ANAMMOX biofilm processes at 18 ± 3 °C was achieved by adding enhanced ANAMMOX granules (LT-granules) into the inoculated denitrification sludge. The results showed that the addition of LT-granules significantly reduced the duration of the three start-up phases (cell lysis phase, activity lag phase, and activity elevation phase) of reactor R2 compared with the control group R1 without LT-granules. It was demonstrated that LT-granules released high contents of N-hexanoyl-DL-homoserine lactone (C6-HSL), N-octanoyl-DL-homoserine lactone (C8-HSL), and N-3-oxohexanoyl-L-homoserine lactone (3OC6-HSL). The C6-HSL and C8-HSL from LT-granules were strongly positively correlated with the concentrations of polysaccharides (TB-PS) and proteins (TB-PN) in tightly bound extracellular polymeric substances (TB-EPS) in R2 biofilms, respectively. Thus, LT-granules promoted the release of TB-PS and TB-PN from the biofilm in R2 during activity lag and activity elevation phases, improving the biofilm adhesion performance. Furthermore, it was proved that the C6-HSL, C8-HSL, and 3OC6-HSL from LT-granules significantly stimulated the relative abundance of Candidatus Brocadia genus and the expression of functional genes hzo and hzsA in R2 biofilms during activity lag and activity elevation phases. These are the main reasons why adding LT-granules promoted the start-up of reactor R2 at 18 ± 3 °C effectively. This study is the first work to accelerate the start-up of the ANAMMOX biofilm system at the low temperature by the economical quorum sensing (QS) regulation based on endogenous N-acyl-homoserine lactone signals (AHLs) and supply a new way for the rapid start-up of ANAMMOX processes in the low-temperature environment.

Rapid determination of 2,4-diaminopyrimidine residues through sample pretreatment using immunomagnetic bead purification along with HPLC-UV.

To reduce matrix interference and realize simultaneous detection of multiple homologous compounds (trimethoprim (TMP), diaveridine (DVD), ormetoprim (OMP), baquiloprim (BQP), and aditoprim (ADP) in pig, cattle, chicken, and fish muscles), an immunomagnetic bead (IMB)-based sample purification pretreatment with HPLC-UV was developed. A broad-spectrum monoclonal antibody (mAb, named 14C6) was prepared and conjugated with carboxylic-acid-functionalized magnetic nanoparticles using the active ester method to obtain IMBs for sample purification. The extraction solvent was optimized based on the extraction efficiency. Good linearity was observed for all the five analytes (10-200 µg/kg) with the LOD and LOQ of 5 and 10 µg/kg, respectively. The mean recoveries ranged from 62.5% to 76.9%, while the coefficient of variation was <12.2%. The IMB method afforded greater sample purification and enrichment than those achieved with the SPE column-based conventional method. Hence, the IMB-based sample purification is a useful tool to determine 2,4-diaminopyrimidine residues in edible animal tissues.

Enhancing the treatment performance of partial denitrification/Anammox process at high nitrogen load: Effects of immobilized strain HFQ8C/Non the sludge characteristics.

A novel strategy based on quorum sense (QS) was proposed to improve the treatment performance of the partial denitrification/Anammox (PD/A) process at high loads by adding immobilized Pseudomonas sp. HFQ8C/N, which could release high concentrations of N-butyryl-DL-homoserine lactone (C4-HSL), N-octanoyl-DL- homoserine lactone (C8-HSL) and N-decanoyl-DL-homoserine lactone (C10-HSL). The results showed that adding immobilized HFQ8C/N improved the sludge activity and settleability, contributing to higher nitrogen removal efficiency at the high nitrogen loading rate (NLR). It was proved that C4-HSL promoted the abundances of Thauera and Candidatus Kuenenia at NLR 1.68-2.52 kg N/(m3·d), while C10-HSL promoted the abundance of Candidatus Brocadia. Besides, C8-HSL and C10-HSL played different regulation roles in the production of protein (PN) in tightly bound extracellular polymeric substances (TB-EPS) at different loads, improving the sludge settleability. This study provided a new way to improve the treatment performance of high-load PD/A processes.