High Sensitivity Label-Free Quantitative Method for Detecting Tumor Biomarkers in Human Serum by Optical Microfiber Couplers.

Label-free optical fiber immunosensors have attracted widespread attention in recent decades due to their high sensitivity. However, nonspecific adsorption in serum has remained a critical bottleneck in existing label-free fiber optic biosensors, which hinders their widespread use in diagnostics. In addition, individual differences in clinical human serum (HS) negatively impact biosensing results. In this work, the modified serum preadsorption strategy was applied to reduce nonspecific adsorption by forming a saturated antifouling interface on an optical microfiber coupler (OMC). Furthermore, to reduce the effect of the differences between individual HS samples, we proposed a new method where Sigma HS was used as a wavelength shift reference due to being close to clinical serum compared to other serums. Sigma HS was used first to reduce the differences in immune sensors before performing a clinical sample test in which quantitative detection was achieved based on the independent calibration of several sensors with wide dynamic ranges via dissociation processes. The individual differences in 25% HS were corrected by 30% Sigma HS. As a proof of concept, the label-free OMC immune sensor demonstrates good sensitivity and specificity for the detection of carcinoembryonic antigen (CEA) in 25% Sigma HS at different concentrations. The detection limit of CEA reached as low as 34.6 fg/mL (0.475 fM). Additionally, label-free quantitative detection of CEA using this OMC immune sensor was verified experimentally according to the calibration line, and the results agree well with clinical examination detection. To our knowledge, it is the first study to employ an OMC immune sensor in point-of-care label-free quantitative detection for clinical HS.

Ultrasensitive interferometers based on zigzag-shaped tapered optical microfibers operating at the dispersion turning point.

This work proposes and demonstrates a novel interferometric sensor based on a zigzag-shaped tapered optical microfiber (Z-OMF) working at the dispersion turning point (DTP). The Z-OMF can be fabricated in a controllable manner through a modified fiber tapering method. Our study shows that the bending taper can transfer a portion of the fundamental HE11 mode to higher-order modes, and when the bending angle of the Z-OMF reaches 1.61°, high contrast interference fringes can be formed between the HE11 and the HE21 modes. More importantly, we find that by optimizing the diameter of the OMF, the group effective refractive index (RI) difference between HE11 and HE21 mode equals zero, and the refractive index sensing performance can be dramatically improved. To validate our proposed sensing mechanism, we experimentally demonstrate an ultrahigh sensitivity of 1.46×105 ± 0.09×105 nm/RIU. The proposed Z-OMF interferometer has the advantage of high sensitivity and low cost and shows excellent potential in chemical and biological detection.

Ultrasensitive label-free optical microfiber coupler biosensor for detection of cardiac troponin I based on interference turning point effect.

Sensitive detection of cardiac biomarkers is critical for clinical diagnostics of myocardial infarction (MI) while such detection is quite challenging due to the ultra-low concentration of cardiac biomarkers. In this work, a label-free immunosensor based on optical microfiber coupler (OMC) has been developed for the ultrasensitive detection of cardiac troponin I (cTnI), a selective and highly sensitive biomarker of acute myocardial infarction (AMI). CTnI monoclonal antibodies were immobilized on the surface of the fiber through polyelectrolyte layer using layer-by-layer deposition technique. For refractive index sensing characterization, an ultra-high sensitivity of 91777.9 nm/RIU was achieved when the OMC works around the dispersion turning point, which is the highest experimental demonstration in the field of fiber-optic evanescent biosensors. For biosensing, the immunosensor with good specificity showed a linear wavelength shift in the range of 2-10 fg/mL and an ultra-low detection limit of 2 fg/mL. Such immunosensors have huge application potential for the detection of cardiac biomarkers of myocardial infarction due to simple detection scheme, quick response time, ease of handling and miniaturation.