The impact of FAK gene silencing on the proliferation and migration of human tongue squamous cell carcinoma cell lines CAL-27 / 实用医学杂志

Objective To explore the effect of silencing focal adhesion kinase (FAK) gene on the proliferation and migration of human tongue squamous cell carcinoma cell lines CAL-27.Methods The siRNA interference technology was used to complete the construction of FAK siRNA by transient transfection.CAL-27 cells were divided into the experiment group,the negative control group and blank control group.The expressions of FAK mRNA and protein were detected by qPCR and Western blotting respectively.The cell proliferation ability was tested by using MTT assay.The cell migration ability was detected by using Transwell chamber assay.Results The expression level of FAK mRNA and protein in experiment group was lower than that in blank control group and negative control group,the difference was significant (PinRNA ＜ 0.01,Pprolein ＜ 0.05).Compared with negative control group and blank control group,the proliferation ability of cells in experiment group was obviously decreased at 48 h and 72 h (P ＜ 0.01).Transwell chamber migration assay showed,the average number of migrating cells in experimnet group group was lower than that in blank control group and negative control group,the difference was significant (P ＜ 0.05).Conclusions FAK gene silencing can significantly inhibit the proliferation ability and migration ability of human tongue squamous cell carcinoma cell lines CAL-27.

SOD/catalase mimetic platinum nanoparticles inhibit heat-induced apoptosis in human lymphoma U937 and HH cells.

Platinum nanoparticles (Pt-NPs) are known to possess anti-tumouric activity and the ability to scavenge superoxides and peroxides indicating that they can act as superoxide dismutase (SOD)/catalase mimetics. These potentials seem useful in the protection and/or amelioration of oxidative stress-associated pathologies, but, when they are combined with a therapeutic modality that depends upon the mediation of reactive oxygen species in cell killing induction, the effect of Pt-NPs might be questionable. Here, the effects of polyacrylic acid-capped Pt-NPs (nano-Pts) on hyperthermia (HT)-induced apoptosis and the underlying molecular mechanisms were investigated in human myelomonocytic lymphoma U937 and human cutaneous T-cell lymphoma HH cells. The results showed that the pre-treatment with nano-Pts significantly inhibited HT-induced apoptosis in a dose-dependent manner. Superoxide, but not peroxides, was suppressed to varying extents. All pathways involved in apoptosis execution were also negatively affected. The results reveal that the combination of nano-Pts and HT could result in HT-desensitization.