Shenfu injection protects human ECV304 cells from hydrogen peroxide via its anti-apoptosis way.

ETHNOPHARMACOLOGICAL RELEVANCE: The pathogenesis of thromboangiitis obliterans (TAO) has not been fully elucidated until now. Shenfu injection (SFI), a traditional Chinese formula has been widely used clinically for the treatment of cardiovascular diseases for more than two decade. Our previous results first suggested that SFI can cause a significant therapeutic effect on experimental TAO model rats. This experiment was designed to further investigate the protective effect of SFI on VEC damaged by hydrogen peroxide (H2O2) oxidative stress in vitro. METERIALS AND METHODS: The cell viability was evaluated by the MTT assay, the activities of SOD and GSH-PX and the content of MDA in the supernatants of the cultured ECV304 cells were evaluated by a colorimetry method, cell apoptosis was detected by flow cytometry and an AO/EB double staining method. The protein expressions of Bcl2, Bax and caspase-3 were examined by Western blotting. RESULTS: When compared with control group, lower survival rate of ECV304 cells was observed in H2O2 group (p<0.01) ; 20µl/ml, 30µl/ml and 40µl/ml SFI increased the survival rate of ECV304 cells under H2O2 oxidative stress (p<0.05 and p<0.01). The activities of SOD and GSH-PX were higher and MDA level was lower in H2O2 group than those in control group. These effects of H2O2 on SOD, GSH-PX activities and MDA content were reversed by SFI in concentration-dependent way (p<0.05 and p<0.01). Flow cytometry and AO-EB double staining discovered that SFI pretreatment inhibited the ECV304 cells apoptosis. The protein expression of caspase3 in 30µl/ml and 40µl/ml SFI groups significantly decreased whereas Bcl2 protein expressions in 20µl/ml, 30µl/ml and 40µl/ml SFI groups were higher than H2O2 group, with Bax protein expression much lower than H2O2 group (p<0.05 and p<0.01). CONCLUSIONS: Our findings suggest that SFI could prevent the ECV304 cells against H2O2 oxidative-stress by enhancing antioxidant enzyme activities, reducing the membrane lipid peroxidation, as well as upregulating antiapoptotic and downregulating apoptosis protein expressions.

Achievement of ABC goals and thrir association with serum creatinine and urea nitrogen among community middle aged and elderly diabetics / 上海预防医学

Objective To investigate the achievement of HbAlc ( A) ,blood pressure ( B) ,and LDL-C ( C) control goals of diabetes and explore their association with indices of renal function among diabetics in community. Methods A total of 388 diabetes patients aged 45 years and older were enrolled who received physical examination in a community.In addition to blood pressure (BP),were measured fasting blood glucose,glycated hemoglobin ( HbAlc) ,serum lipid,creatinine,and urea nitrogen. Results In this investigation only 1.8%of diabetes patients achieved ABC goals.And 34.0% achieved none of the three and 45.9%reached only one of the three.In the 388 diabetes patients,54.6%had HbAlc<7%,25.0%LDL-C <2.6 mmol/L,and 8.2% BP<130/80 mmHg.The levels of serum creatinine and urea nitrogen were elevated with age and not found to have association with the achievement of ABC goals. Conclusion Poor achievement of ABC goals was among diabetics in community.The effective control of blood pressure and LDL contributes to improvement of comprehensive control levels for diabetics.

Estimating risk of stroke in elderly people by use of Framingham Stroke Risk Profile / 上海预防医学

Objective To explore the application of Framingham Stroke Risk Profile in elderly people. Methods A total of 5 148 people aged 55 years and older who received physical examination in a community were enrolled.Information of their healthy status and risk factors were collected by interview using a questionnaire.Height, weight, waist circumference (WC), and blood pressure as well as fasting plasma glucose ( FPG) , and serum lipid were measured. Results The average 10-year probabilities of stroke in elderly people were 9.79% in men and 6.20% in women ( P <0.05).Besides aging factor, higher risk probability in men might be related to their higher proportions of smoking and atrial fibrillation. In addition to blood pressure and FPG which were variables associated with risk profile, WC/height ratio ( WHR) and serum triglyceride level were associated with increased risk of stroke estimated by stroke risk profile (OR=1.598, 95%CI:1.371~1.864;OR=1.487, 95%CI:1.225~1.783), respectively. Conclusion Besides factors which had been included by risk profile, it is important to pay attention to weight control and triglyceride level.For elderly male, to quit smoking is an important measure that decreases their risk of stroke.