Mechanism of Fluspirilene suppressing HCC through decreasing Akt expression / 中国药理学通报

Aim To explore the mechanism of Fluspirilene inhibiting HCC through decreasing the expression of Akt.Methods The difference of mRNA was verified by the test of protein expression between Fluspirilenc treatment group and control group by HCC experiment in vivo and vitro, including Western blot, IHC after mRNA array.Results Akt expression was lower in Fluspirilene treatment group than that in control group by mRNA array.Protein expression of Akt, phosphorylate-CDK2 and phos- phorylate-Rb decreased massively in Fluspirilene treatment group in a concentration-dependent manner in HepG2 and Huh7 cells by Western blotting compared with those in control group.Declined expression of phosphorylate-Akt was proved in a concen- tration-dependent manner in xenograft tumor tissues in Fluspirilene treatment group compared with that in control group in IHC test.Conclusions Fluspirilene inhibits HCC by decreasing significantly the protein expression of Akt, phosphorylat-Akt, phos- phorylate-CDK2 and phosphorylate-Rb.

Histopathologic features of rim enhancement MRI for breast infiltrating ductal carcinoma / 第二军医大学学报

Objective: To investigate the histopathologic features of rim enhancement MRI of breast infiltrating ductal carcinoma (IDC). Methods: Routine and dynamic contrast enhanced MRI was used to examine 65 patients with breast lumps. Thirty of the 65 patients who were pathologically confirmed to have breast IDC were included in the present study. The manifestations of dynamic contrast enhanced MRI and the histopathologic parameters of the masses (the size of cancer nest, stroma type, microvessel density and degree of fibrosis) were observed. And the relationship of MR findings with the above-mentioned histopathologic features was analyzed. Results: Peripheral rim enhancement was obvious in IDC and a type-III (washout) time/signal intensity course was the dominant type (17/30,56.7%). The average enhancement rate during the first post-contrast minute(ΔSI1 %) was higher than 75% in IDC. The 30 IDCs fell into small (13,43%), medium(12,40%), and large (5,17%) groups according to the size of cancer nest; and into delicate(5,17%), narrow(16,53%), and broad(9,30%) groups according to the cancer stroma type. Early rim enhancement was associated with a small cancer nest (P<0.05), a high ratio of peripheral-to-central microvessel density, and a low ratio of peripheral-to-central fibrosis (P<0.01). Delayed rim enhancement was significantly associated with narrow stroma (P<0.05). Conclusion: Rim enhancement and washout sign on contrast-enhanced MR imaging of the breast IDC are associated not only with angiogenesis, but also with various histological features of the cancinoma, including the size of cancer nests, width of stroma, and degree of fibrosis.

Clinical significance of cervical vertebral flexion and extension spatial alignment changes.

STUDY DESIGN: Radiographic review. OBJECTIVE: To study changes in alignment and curvature of the cervical vertebral column in the sagittal plane, and to provide references for the orthopedic treatment of cervical kyphosis. SUMMARY AND BACKGROUND DATA: There are few reports analyzing changes of the vertebral body itself or changes in their relationships between separate vertebrae. To understand cervical vertebrae and curvature dynamic variation patterns in the sagittal plane and provide a theoretical reference for orthopedic correction of cervical kyphosis, we measured spatial position and alignment of the cervical vertebrae on sagittal plane films of various postures. METHOD: Blind repeated measures design analysis of sagittal view standard lateral, hyper flexion, and hyper extension cervical spine of 60 healthy subjects measuring spatial alignment angle of C2 to C7 vertebral bodies in the sagittal plain ( angleA), C2 to C7 inferior terminal lamina tilt angle of ( angleB), and segmental intervertebral space angles C2/3 to C6/7 ( angleC) calculating flexion and extension amplitude changes in angleA, angleB, and angleC. Cervical curve apex was determined using Borden's method to compare change and distribution characteristics. RESULTS.: Segmental angleA is a positive mean value that increases from C2 to C7 when in the upright position. In hyper extension, mean angleA value decreases with gradual amplitude decrease from C2 to C7; however, a gradual overall increasing pattern is observed for mean angleA from C2 to C7. In hyper flexion, segmental angleA mean value increases with gradual amplitude decrease from C2 to C7, whereas with decreasing angular measurements from C2 to C7. angleB follows similar change regularities as angleA with a larger mean value than angleA. During maximum extension and maximum flexion, angleD is equal to angleE. For example: C2 angleD = C2 variable angleA; C2 angleE angle(C3 variable angleA + C2/3 variable angleC); and C2 angleD = C2 angleE. Cervical spine curve apex is mainly distributed between C4 and C5 on standard, hyper extension and hyper flexion lateral view. CONCLUSION: Dynamic cervical curvature changes based on a central apex, stems from vertebral rotation and displacement in the sagittal plane. Our study reveals variation patterns of dynamic cervical spine sagittal alignment and curvature, providing vertebral spatial alignment value as reference for orthopedic cervical kyphosis corrective surgery.